Development and characterization of 38 polymorphic microsatellite markers from an economically important fruit tree, the Indian jujube.

Abstract:

PREMISE OF THE STUDY:A total of 38 polymorphic microsatellite loci of the Indian jujube (Ziziphus mauritiana), an economically important fruit tree, were developed to evaluate genetic diversity and aid in the identification of cultivars. METHODS AND RESULTS:The 38 microsatellite markers were isolated from the Indian jujube using a magnetic bead enrichment method, and polymorphisms were identified in 24 Indian jujube cultivars. The number of alleles ranged from two to 13, with expected heterozygosity ranging from 0.261 to 0.898. The polymorphism information content ranged from 0.248 to 0.889, with a mean of 0.616. Of these 38 simple sequence repeat loci, 20 loci from Z. jujuba (Chinese jujube) were successfully amplified using the simple sequence repeat primer sets. CONCLUSIONS:These polymorphic loci should be useful in further studies of the genetic diversity and the identification of cultivars of both the Indian jujube and the Chinese jujube.

journal_name

Am J Bot

authors

Chiou CY,Chiang YC,Chen CH,Yen CR,Lee SR,Lin YS,Tsai CC

doi

10.3732/ajb.1100500

subject

Has Abstract

pub_date

2012-05-01 00:00:00

pages

e199-202

issue

5

eissn

0002-9122

issn

1537-2197

pii

ajb.1100500

journal_volume

99

pub_type

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