Development and characterization of microsatellite loci in Pericopsis elata (Fabaceae) using a cost-efficient approach.

Abstract:

PREMISE OF THE STUDY:Microsatellite loci were developed in the endangered Pericopsis elata using a combination of low-cost procedures. METHODS AND RESULTS:Microsatellite isolation was performed simultaneously on three distinct species through a newly available procedure that associates multiplex microsatellite enrichment and next-generation sequencing, allowing the rapid and low-cost development of microsatellite-enriched libraries through the use of a 1/32nd GS-FLX plate. Genotyping using M13-like labeling in multiplexed reactions allowed additional cost savings. From 72 primers selected for initial screening, 21 positively amplified P. elata, and 11 showed polymorphism with two to 11 alleles per locus and a mean value of 5.4 alleles per locus. CONCLUSIONS:These microsatellite loci will be useful to further investigate the level of genetic variation within and between natural populations of P. elata in Africa.

journal_name

Am J Bot

authors

Micheneau C,Dauby G,Bourland N,Doucet JL,Hardy OJ

doi

10.3732/ajb.1100070

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

e268-70

issue

10

eissn

0002-9122

issn

1537-2197

pii

ajb.1100070

journal_volume

98

pub_type

杂志文章
  • IRIS NELSONII (IRIDACEAE): ORIGIN AND GENETIC COMPOSITION OF A HOMOPLOID HYBRID SPECIES.

    abstract::Nuclear and chloroplast DNA variation was assayed for two populations of Louisiana irises (Bayou Teche and Young's Coulee) that demonstrated extreme morphological variation and for a sample of the putative hybrid species, Iris nelsonii. The genetic markers examined in this analysis were diagnostic for either Iris fulv...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/j.1537-2197.1993.tb13843.x

    authors: Arnold ML

    更新日期:1993-05-01 00:00:00

  • Structural correlates of imbibitional injury in Typha pollen.

    abstract::The ultrastructure of Typha latifolia pollen was examined as a function of pollen moisture content and incubation temperature, in order to identify possible lesions induced by imbibitional chilling. A syndrome of structural traits was found which characterizes damaged grains. Compared to viable grains, the protoplas...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:

    authors: Sack FD,Leopold AC,Hoekstra FA

    更新日期:1988-01-01 00:00:00

  • Genetic diversity of Typha latifolia (Typhaceae) and the impact of pollutants examined with tandem-repetitive DNA probes.

    abstract::Genetic diversity at variable-number-tandem-repeat (VNTR) loci was examined in the common cattail, Typha latifolia (Typhaceae), using three synthetic DNA probes composed of tandemly repeated "core" sequences (GACA, GATA, and GCAC). The principal objectives of this investigation were to determine whether: (1) the previ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:

    authors: Keane B,Pelikan S,Toth GP,Smith MK,Rogstad SH

    更新日期:1999-09-01 00:00:00

  • Bryophyte diaspore bank: a genetic memory? Genetic structure and genetic diversity of surface populations and diaspore bank in the liverwort Mannia fragrans (Aytoniaceae).

    abstract::Propagule banks are assumed to be able to store considerable genetic variability. Bryophyte populations are expected to rely more heavily on stored propagules than those of seed plants due to the vulnerability of the haploid gametophyte. This reliance has important implications for the genetic structure and evolutiona...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.2007283

    authors: Hock Z,Szövényi P,Schneller JJ,Tóth Z,Urmi E

    更新日期:2008-05-01 00:00:00

  • Mixed mating in a recently derived self-compatible population of Leavenworthia alabamica (Brassicaceae).

    abstract:UNLABELLED: PREMISE OF THE STUDY:A mixture of outcrossing and selfing is often observed in plant populations. Although mixed mating is ubiquitous, it has several potential evolutionary explanations. Mixed mating may be actively maintained by selection, passively determined by the pollination environment, or a transiti...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1000032

    authors: Busch JW,Herlihy CR,Gunn L,Werner WJ

    更新日期:2010-06-01 00:00:00

  • The pollination biology of two paniculate agaves (Agavaceae) from northwestern Mexico: contrasting roles of bats as pollinators.

    abstract::Paniculate agaves from tropical deserts depend on nectar-feeding bats for their reproduction, while species from extratropical areas near the northern limit of Leptonycteris curasoae are pollinated by diurnal and nocturnal insects and birds. Agave angustifolia is a paniculate agave with a broad distribution in Mexico ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.90.7.1016

    authors: Molina-Freaner F,Eguiarte LE

    更新日期:2003-07-01 00:00:00

  • The pollination ecology of buzz-pollinated Rhexia virginica (Melastomataceae).

    abstract::We examined the function of floral traits associated with buzz pollination through studies of Rhexia virginica (Melastomataceae) in the Muskoka region of Ontario, Canada. Controlled pollinations demonstrated that the species is self-compatible, but dependent on insects for pollen transfer. Bumble bees made 82 and 90% ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:

    authors: Larson BM,Barrett SC

    更新日期:1999-04-01 00:00:00

  • Systematics of Amaryllidaceae based on cladistic analysis of plastid sequence data.

    abstract::Cladistic analyses of plastid DNA sequences rbcL and trnL-F are presented separately and combined for 48 genera of Amaryllidaceae and 29 genera of related asparagalean families. The combined analysis is the most highly resolved of the three and provides good support for the monophyly of Amaryllidaceae and indicates Ag...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:

    authors: Meerow AW,Fay MF,Guy CL,Li QB,Zaman FQ,Chase MW

    更新日期:1999-09-01 00:00:00

  • A case study of modified interactions with symbionts in a hybrid mediterranean orchid.

    abstract:UNLABELLED: PREMISE OF THE STUDY:Most studies on orchid hybrids examine separately the effects of hybridization on interactions with pollinators or with mycorrhizal fungi. Here, we simultaneously investigated both interactions in the mediterranean food-deceptive Orchis simia, O. anthropophora, and their hybrid (O. ×be...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.0900303

    authors: Schatz B,Geoffroy A,Dainat B,Bessière JM,Buatois B,Hossaert-McKey M,Selosse MA

    更新日期:2010-08-01 00:00:00

  • Influence of plant resistance traits in selectiveness and species strength in a tropical plant-herbivore network.

    abstract:PREMISE OF THE STUDY:Plant-herbivore networks are highly specialized in their interactions, yet they are highly variable with regard to the relative importance of specific host species for herbivores. How host species traits determine specialization and species strength in this antagonistic network is still an unanswer...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1600045

    authors: López-Carretero A,Boege K,Díaz-Castelazo C,Domínguez Z,Rico-Gray V

    更新日期:2016-08-01 00:00:00

  • Habitat fragmentation threatens wild populations of Carica papaya (Caricaceae) in a lowland rainforest.

    abstract::• Premise of the study: Wild populations of domesticated species constitute a genetic reservoir and are fundamental to the evolutionary potential of species. Wild papaya (Carica papaya) is a rare, short-lived, gap-colonizing, dioecious tree that persists in the forest by continuous dispersal. Theoretically, these life...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1400051

    authors: Chávez-Pesqueira M,Suárez-Montes P,Castillo G,Núñez-Farfán J

    更新日期:2014-07-01 00:00:00

  • Estimation of temperature and precipitation from morphological characters of dicotyledonous leaves.

    abstract::The utility of regression and correspondence models for deducing climate from leaf physiognomy was evaluated by the comparative application of different predictive models to the same three leaf assemblages. Mean annual temperature (MAT), mean annual precipitation (MAP), and growing season precipitation (GSP) were esti...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:

    authors: Wiemann MC,Manchester SR,Dilcher DL,Hinojosa LF,Wheeler EA

    更新日期:1998-12-01 00:00:00

  • Lax midrib1-O, a systemic, heterochronic mutant of maize.

    abstract::Lxm1-O, a dominant EMS (ethyl methanesulfonate) induced mutation in maize (Zea mays, Poaceae), was originally reported to affect the blade/sheath boundary over the midrib region of the leaf. Here we present a more extensive analysis of the Lxm phenotype in nine different inbred lines. Lxm leaves are longer and narrowe...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:

    authors: Schichnes D,Freeling M

    更新日期:1998-04-01 00:00:00

  • Cuscuta jepsonii (Convolvulaceae): An invasive weed or an extinct endemic?

    abstract::Despite their ecological significance, parasitic plants face more conservation challenges than do autotrophic plants. This is especially true for the groups that include weedy or invasive species such as Cuscuta. While approximately half of the Cuscuta (dodders) species may require conservation measures, the genus as ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.0800425

    authors: Costea M,Stefanovic S

    更新日期:2009-09-01 00:00:00

  • Climatic niche comparison among ploidal levels in the classic autopolyploid system, Galax urceolata.

    abstract:PREMISE OF THE STUDY:Autopolyploidy, or whole-genome duplication within a species, leads to closely related cytotypes in one geographic location. One hypothesized mechanism by which autopolyploids become established is climatic niche divergence from their diploid progenitor. Here we tested this hypothesis in diploid, t...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/ajb2.1161

    authors: Gaynor ML,Marchant DB,Soltis DE,Soltis PS

    更新日期:2018-10-01 00:00:00

  • The role of auxin transport during inflorescence development in maize (Zea mays, Poaceae).

    abstract::Axillary meristems play a fundamental role in inflorescence architecture. Maize (Zea mays) inflorescences are highly branched panicles because of the production of multiple types of axillary meristems. We used auxin transport inhibitors to show that auxin transport is required for axillary meristem initiation in the m...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.94.11.1745

    authors: Wu X,McSteen P

    更新日期:2007-11-01 00:00:00

  • Life span correlates with population dynamics in perennial herbaceous plants.

    abstract::Survival and fecundity are basic components of demography and therefore have a strong influence on population dynamics. These two key parameters and their relationship are crucial to understand the evolution of life histories. It remains, however, to be empirically established how life span, fecundity, and population ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.95.2.258

    authors: García MB,Picó FX,Ehrlén J

    更新日期:2008-02-01 00:00:00

  • Estimating near-infrared leaf reflectance from leaf structural characteristics.

    abstract::The relationship between near-infrared reflectance at 800 nm (NIRR) from leaves and characteristics of leaf structure known to affect photosynthesis was investigated in 48 species of alpine angiosperms. This wavelength was selected to discriminate the effects of leaf structure vs. chemical or water content on leaf ref...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:

    authors: Slaton MR,Raymond Hunt E Jr,Smith WK

    更新日期:2001-02-01 00:00:00

  • The structure and development of incompletely closed carpels in an apocarpous species, Sagittaria trifolia (Alismataceae).

    abstract::• Premise of the study: Carpel closure is commonly considered as a key innovation in angiosperms; however, some families continue to exhibit a variety of forms of incomplete carpel closure. The carpel of Sagittaria species contains an unusual structure. In this study, we confirm the closure type of the carpel of Sagit...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1400172

    authors: Huang LJ,Wang XW,Wang XF

    更新日期:2014-07-01 00:00:00

  • The age and diversification of terrestrial New World ecosystems through Cretaceous and Cenozoic time.

    abstract::Eight ecosystems that were present in the Cretaceous about 100 Ma (million years ago) in the New World eventually developed into the 12 recognized for the modern Earth. Among the forcing mechanisms that drove biotic change during this interval was a decline in global temperatures toward the end of the Cretaceous, augm...

    journal_title:American journal of botany

    pub_type: 杂志文章,评审

    doi:10.3732/ajb.1000353

    authors: Graham A

    更新日期:2011-03-01 00:00:00

  • Paternal inheritance of chloroplast DNA in interspecific hybrids in the genus Larrea (Zygophyllaceae).

    abstract::The mode of chloroplast DNA (cpDNA) inheritance was investigated in the genus Larrea (Zygophyllaceae) by polymerase chain reaction (PCR) amplification of cpDNA fragments using three pairs of chloroplast universal primers. A total of 20 F(1)s from interspecific crosses among five different taxa in the section Bifolium ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:

    authors: Yang TW,Yang YA,Xiong Z

    更新日期:2000-10-01 00:00:00

  • Seasonal changes in physiological performance in wild Clarkia xantiana populations: Implications for the evolution of a compressed life cycle and self-fertilization.

    abstract:PREMISE OF THE STUDY:One explanation for the evolution of selfing, the drought escape hypothesis, proposes that self-fertilization may evolve under conditions of intensifying seasonal drought as part of a suite of traits that enable plants to accelerate the completion of their life cycle, thereby escaping late-season d...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1400557

    authors: Dudley LS,Hove AA,Emms SK,Verhoeven AS,Mazer SJ

    更新日期:2015-06-01 00:00:00

  • Chloroplast microsatellite primers for cacao (Theobroma cacao) and other Malvaceae.

    abstract:PREMISE OF THE STUDY:Chloroplast microsatellites were developed in Theobroma cacao to examine the genetic diversity of cacao cultivars in Trinidad and Tobago. METHODS AND RESULTS:Nine polymorphic microsatellites were designed from the chloroplast genomes of two T. cacao accessions. These microsatellites were tested in...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1100306

    authors: Yang JY,Motilal LA,Dempewolf H,Maharaj K,Cronk QC

    更新日期:2011-12-01 00:00:00

  • New insights into the phylogenetic relationships, character evolution, and phytogeographic patterns of Calceolaria (Calceolariaceae).

    abstract::Biogeographical patterns and diversification processes in Andean and Patagonian flora are not yet well understood. Calceolaria is a highly diversified genus of these areas, representing one of the most specialized plant-pollinator systems because flowers produce nonvolatile oils, a very unusual floral reward. Phylogen...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.0900165

    authors: Cosacov A,Sérsic AN,Sosa V,De-Nova JA,Nylinder S,Cocucci AA

    更新日期:2009-12-01 00:00:00

  • Development of microsatellite loci for the endangered species Pityopsis ruthii (Asteraceae).

    abstract:PREMISE OF THE STUDY:Microsatellite loci were developed for the endangered species Pityopsis ruthii and will permit genetic and conservation studies of the species. METHODS AND RESULTS:A microsatellite-enriched library was used to develop 12 polymorphic microsatellite loci for P. ruthii. The loci amplified perfect and...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1100100

    authors: Wadl PA,Dattilo AJ,Scheffler BE,Trigiano RN

    更新日期:2011-12-01 00:00:00

  • Leaf dimorphism in Archaeopteris roemeriana (Progymnosperm): further early fossil evidence of shoot dorsiventrality.

    abstract::Additional information on the morphology of the vegetative ultimate branches of Archaeopteris roemeriana was obtained by uncovering compression specimens collected in Upper Devonian deposits in Belgium. For the first time, anisophylly, previously inferred from anatomical studies, is demonstrated on one species of the ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:

    authors: Fairon-Demaret M,Leponce I

    更新日期:2001-04-01 00:00:00

  • Complete life cycle of the lichen fungus Calopadia puiggarii (Pilocarpaceae, Ascomycetes) documented in situ: propagule dispersal, establishment of symbiosis, thallus development, and formation of sexual and asexual reproductive structures.

    abstract:PREMISE OF THE STUDY:The life histories of lichen fungi are not well known and cannot be readily studied in laboratory culture. This work documents in situ the complete life cycle of the widespread crustose lichen Calopadia puiggarii, which reproduces sexually and asexually on the surfaces of leaves. METHODS:Plastic c...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1400272

    authors: Sanders WB

    更新日期:2014-11-01 00:00:00

  • The taxonomic value of fruit wing types in the order Apiales.

    abstract::This study shows that structural data, when carefully examined, can provide valuable characters for delimiting monophyletic groups and can complement DNA with observable features to recognize and circumscribe taxa. In the angiosperm order Apiales, traditional classification has relied heavily (often exclusively) on fr...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.93.9.1357

    authors: Liu MR,Plunkett GM,Lowry PP 2nd,Wyk BE,Tilney PM

    更新日期:2006-09-01 00:00:00

  • Foliar secretory structures in Crotoneae (Euphorbiaceae): Diversity, anatomy, and evolutionary significance.

    abstract:PREMISE OF THE STUDY:Phylogenetic and morphological studies have helped clarify the systematics of large and complex groups such as the tribe Crotoneae (Euphorbiaceae). However, very little is known about the diversity, structure, and function of anatomical features in this tribe. Crotoneae comprises the species-rich p...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1500017

    authors: Vitarelli NC,Riina R,Caruzo MB,Cordeiro I,Fuertes-Aguilar J,Meira RM

    更新日期:2015-06-01 00:00:00

  • Immunohistochemical investigation of the necrotrophic phase of the fungus Colletotrichum gloeosporioides in the biocontrol of hemp sesbania (Sesbania exaltata; Papilionaceae).

    abstract:UNLABELLED: PREMISE OF THE STUDY:Fungal plant pathogens exert much of their effect on plant cells through alterations in the host cell walls. However, obtaining biochemical proof for this change is difficult because of the relatively small number of cells that are affected by the pathogen relative to the bulk of host ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1000099

    authors: Bowling AJ,Vaughn KC,Hoagland RE,Stetina K,Boyette CD

    更新日期:2010-12-01 00:00:00