Development and characterization of microsatellite loci for Khaya senegalensis (Meliaceae)1.

Abstract:

UNLABELLED: PREMISE OF THE STUDY:Microsatellite loci were developed to characterize genetic variation and population subdivision in Khaya senegalensis (Desr.) A. Juss. (Meliaceae). • METHODS AND RESULTS:Microsatellite loci were identified from genomic DNA sequences generated using the 454 GS-FLX titanium platform. Primers were designed for 67 tri- and tetranucleotide repeats, of which 20 were selected for 2 multiplexes based on amplification success and band size. Eleven of these loci showed polymorphism in two populations of Khaya senegalensis and amplified in individuals from across the species range. • CONCLUSIONS:These new microsatellite loci will be useful for investigation of the evolutionary and conservation genetics of Khaya senegalensis.

journal_name

Am J Bot

authors

Sexton GJ,Frere CH,Dieters MJ,Godwin ID,Prentis PJ

doi

10.3732/ajb.1000300

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

e111-3

issue

11

eissn

0002-9122

issn

1537-2197

pii

ajb.1000300

journal_volume

97

pub_type

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