Characterization of 42 polymorphic microsatellite loci in Mimulus ringens (Phrymaceae) using Illumina sequencing.

Abstract:

UNLABELLED: PREMISE OF THE STUDY:Microsatellite markers were isolated and characterized in Mimulus ringens (Phrymaceae), a herbaceous wetland perennial, to facilitate studies of mating patterns and population genetic structure. • METHODS AND RESULTS:A total of 42 polymorphic loci were identified from a sample of 24 individuals from a single population in Ohio, USA. The number of alleles per locus ranged from two to nine, and median observed heterozygosity was 0.435. • CONCLUSIONS:This large number of polymorphic loci will enable researchers to quantify male fitness, patterns of multiple paternity, selfing, and biparental inbreeding in large natural populations of this species. These markers will also permit detailed study of fine-scale patterns of genetic structure.

journal_name

Am J Bot

authors

Nunziata SO,Karron JD,Mitchell RJ,Lance SL,Jones KL,Trapnell DW

doi

10.3732/ajb.1200180

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

e477-80

issue

12

eissn

0002-9122

issn

1537-2197

pii

ajb.1200180

journal_volume

99

pub_type

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