Conversion of dihomo-gamma-linolenic acid to mono- and dihydroxy acids by potato lipoxygenase: evidence for the formation of 8,9-leukotriene A3.

abstract：:Reports suggest that microRNAs have implications in the development of several diseases including cancer. It is therefore believed that miRs may act as therapeutic targets for cancer treatment. The treatment of ovarian cancer is mainly obstructed by lack of biomarkers and efficient drug targets. Against this backdrop,...

journal_title：Archives of biochemistry and biophysics

authors： Si L,Jia Y,Lin R,Jian W,Yu Q,Yang S

更新日期：2019-06-15 00:00:00

abstract：:Phospholipid transmethylation in the microsomal fraction of stimulated and unstimulated human leukocytes was measured in a recently developed assay system. Microsomal fraction was prepared from neutrophils, unseparated lymphocytes, T lymphocytes, and non-T lymphocytes by sonication and subsequent ultracentrifugation. ...

journal_title：Archives of biochemistry and biophysics

authors： Niwa Y,Sakane T,Taniguchi S

更新日期：1984-10-01 00:00:00

abstract：:X-ray crystallographic studies on glucose oxidase showed a strong interaction between carbohydrate and protein moieties of the glycoprotein. However, experimental studies under physiological conditions reported no influence of carbohydrate moiety on the structural and functional properties of glucose oxidase. In order...

journal_title：Archives of biochemistry and biophysics

authors： Akhtar MS,Bhakuni V

更新日期：2003-05-15 00:00:00

abstract：:Paxilline, an indole alkaloid mycotoxin from Penicillium paxilli, is an inhibitor of the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA). Paxilline inhibited differing isoforms of SERCA with IC50s between 5 and 50 microM. It inhibited more potently the purified Ca2+ ATPase activity from skeletal muscle with an IC50 of...

journal_title：Archives of biochemistry and biophysics

authors： Bilmen JG,Wootton LL,Michelangeli F

更新日期：2002-10-01 00:00:00

abstract：:Oxidation of [2,3-14C]succinate carbons in the mitochondrial Krebs cycle was used as a probe to investigate the effects of insulin, epinephrine, glucagon, and 2,4-dinitrophenol (2,4-DNP) on isolated rat hepatocytes. Epinephrine, glucagon, and 2,4-DNP had a far greater stimulatory effect on 14CO2 formation from [2,3-14...

journal_title：Archives of biochemistry and biophysics

authors： Mohan C,Memon RA,Bessman SP

更新日期：1991-05-15 00:00:00

abstract：:Methionine is oxidized to methionine sulfoxide by rat liver and kidney microsomes in an O2- and NADPH-dependent manner. In all microsomal assays, no methionine sulfone was detected. Use of a monoclonal antibody to rat liver cytochrome P-450 reductase, various cytochrome P-450 and peroxidase inhibitors, antioxidants, a...

journal_title：Archives of biochemistry and biophysics

authors： Krause RJ,Ripp SL,Sausen PJ,Overby LH,Philpot RM,Elfarra AA

更新日期：1996-09-01 00:00:00

abstract：:Changes in the levels of various molecular species of N-acylethanolamine in CdCl2-administered rat testis were examined. We found that the levels of various N-acylethanolamines including anandamide (N-arachidonoylethanolamine), an endogenous cannabinoid receptor ligand, were dramatically increased in CdCl2-admin-ister...

journal_title：Archives of biochemistry and biophysics

authors： Kondo S,Sugiura T,Kodaka T,Kudo N,Waku K,Tokumura A

更新日期：1998-06-15 00:00:00

abstract：:Previous work has shown that the coupling of the soluble Escherichia coli pyruvate oxidase to a lipid-depleted membrane terminal electron transport system requires the addition of ubiquinone and a neutral lipid fraction (C. Cunningham and L. P. Hager (1975) J. Biol. Chem. 250, 7139-7146). The active factor present in ...

journal_title：Archives of biochemistry and biophysics

authors： Kiuchi K,Hager LP

更新日期：1984-09-01 00:00:00

abstract：:Ovalbumin extracted from oviduct slices incubated with [35S]methionine or [2-3]mannose contained two biosynthetic intermediates, OE and OF (Y. Kato, H. Iwase, and K. Hotta, 1984, J. Biochem. (Tokyo) 95, 455-463). In the present study, these intermediates are further characterized. The 3H dpm/35S dpm ratio of OF labele...

journal_title：Archives of biochemistry and biophysics

authors： Kato Y,Iwase H,Hotta K

更新日期：1986-02-01 00:00:00

abstract：:Sequence alignment of pig mitochondrial NADP-dependent isocitrate dehydrogenase with eukaryotic (human, rat, and yeast) and Escherichia coli isocitrate dehydrogenases reveals that Tyr316 is completely conserved and is equivalent to the E. coli Tyr345, which interacts with the 2'-phosphate of NADP in the crystal struct...

journal_title：Archives of biochemistry and biophysics

authors： Lee P,Colman RF

更新日期：2002-05-01 00:00:00

abstract：:Rat transferrin (rTf), containing one complex glycan, and human transferrin (hTf), containing two complex glycans, were treated with peptide:N-glycosidase F (PNGase) under nondenaturing conditions. Apo rTf with a nonfucosylated standard biantennary glycan, but not its diferric counterpart, yielded satisfactory amounts...

journal_title：Archives of biochemistry and biophysics

authors： Regoeczi E,Bolyos M,Chindemi PA

更新日期：1989-02-01 00:00:00

abstract：:To define the influence of time in culture on gene expression for extracellular matrix proteins we have examined the progression of changes in gene expression for chondrocyte extracellular matrix proteins from the time the chondrocytes are initially isolated from their native cartilaginous matrix through 13 days of hi...

journal_title：Archives of biochemistry and biophysics

authors： Hering TM,Kollar J,Huynh TD,Varelas JB,Sandell LJ

更新日期：1994-10-01 00:00:00

abstract：:Recent studies have shown that the light-dark mediated regulation of the leaf photosynthetic enzyme pyruvate, Pi dikinase results from interconversion between an active nonphosphorylated form of the enzyme and an inactive form phosphorylated on a threonine residue. These phosphorylation and dephosphorylation reactions...

journal_title：Archives of biochemistry and biophysics

authors： Burnell JN,Hatch MD

更新日期：1986-03-01 00:00:00

abstract：:This investigation presents disturbances of the mitochondrial metabolism by arsenite, a hydrophilic dithiol reagent known as an inhibitor of mitochondrial alpha-keto acid dehydrogenases. Arsenite at concentrations of 0.1-1.0 mM was shown to induce a considerable oxidation of intramitochondrial NADPH, NADH, and glutath...

journal_title：Archives of biochemistry and biophysics

authors： Lenartowicz E

更新日期：1990-12-01 00:00:00

abstract：:Tuberculosis remains a leading cause of human mortality. The emergence of strains of Mycobacterium tuberculosis, the causative agent, that are resistant to the major frontline antitubercular drugs increases the urgency for the development of new therapeutic agents. Sequencing of the M. tuberculosis genome revealed the...

journal_title：Archives of biochemistry and biophysics

authors： Ouellet H,Johnston JB,Ortiz de Montellano PR

更新日期：2010-01-01 00:00:00

abstract：:Cytochrome P450 reductase was purified to homogeneity from cell suspension cultures of the opium poppy Papaver somniferum, the enzyme was characterized (K(m) cytochrome c, 8.3 microM; K(m) NADPH, 4.2 microM; pH optimum, 8.0; M(r), 80 kDa), and the amino acid sequence of internal peptides was determined. Partial cDNA c...

journal_title：Archives of biochemistry and biophysics

authors： Rosco A,Pauli HH,Priesner W,Kutchan TM

更新日期：1997-12-15 00:00:00

abstract：:Peroxynitrite, a biological oxidant formed from the reaction of nitric oxide with the superoxide radical, is associated with many pathologies, including neurodegenerative diseases, such as multiple sclerosis (MS). Gout (hyperuricemic) and MS are almost mutually exclusive, and uric acid has therapeutic effects in mice ...

journal_title：Archives of biochemistry and biophysics

authors： Squadrito GL,Cueto R,Splenser AE,Valavanidis A,Zhang H,Uppu RM,Pryor WA

更新日期：2000-04-15 00:00:00

abstract：:Here, we investigated the structure-activity relationship of major green tea catechins and their corresponding epimers on cell-surface binding and inhibitory effect on histamine release. Galloylated catechins; (-)-epigallocatechin-3-O-gallate (EGCG), (-)-gallocatechin-3-O-gallate (GCG), (-)-epicatechin-3-O-gallate (EC...

journal_title：Archives of biochemistry and biophysics

authors： Fujimura Y,Umeda D,Yamada K,Tachibana H

更新日期：2008-08-15 00:00:00

abstract：:In our previous reports [Z. Long, H. Homma, J.-A. Lee, T. Fukushima, T. Santa, T. Iwatsubo, R. Yamada, K. Imai, FEBS Lett. 434 (1998) 231-235; Z. Long, M. Sekine, M. Adachi, T. Furuchi, K. Imai, N. Nimura, H. Homma, Arch. Biochem. Biophys. 404 (2002) 92-97], we demonstrated for the first time that D-aspartate (D-Asp) ...

journal_title：Archives of biochemistry and biophysics

authors： Koyama H,Adachi M,Sekine M,Katane M,Furuchi T,Homma H

更新日期：2006-02-15 00:00:00

abstract：:Troponin T (TnT) binds to tropomyosin (Tm) to anchor the troponin complex in the thin filament, and it thus serves as a vital link in the Ca(2+) regulation of striated muscle contraction. Pioneer work three decades ago determined that the T1 and T2 chymotryptic fragments of TnT each contains a Tm-binding site. A more ...

journal_title：Archives of biochemistry and biophysics

authors： Jin JP,Chong SM

更新日期：2010-08-15 00:00:00

abstract：:Recognition of defined carbohydrate structures by boar sperm was studied on the basis of oligosaccharide structures of porcine zona pellucida glycoproteins so far elucidated. Boar sperm abundantly adhered to fetuin-Sepharose beads, moderately to asialofetuin-Sepharose beads, but not at all to galactosidase (beta1-4-li...

journal_title：Archives of biochemistry and biophysics

authors： Mori E,Yoshitani N,Mori T,Takasaki S

更新日期：2000-02-01 00:00:00

abstract：:The maximal amounts of Ca2+ being accumulated (delta Ca2+max) and H+ emitted (delta H+max) by Ca2+-loading mitochondria, with succinate (+rotenone) as respiratory substrate, were evaluated. delta Ca2+max was increased by providing either citrate or ATP to a Pi- and Mg2+-free medium. With citrate, delta H+max was only ...

journal_title：Archives of biochemistry and biophysics

authors： Guidoux R

更新日期：1985-12-01 00:00:00

abstract：:Sepsis is a life-threatening clinical syndrome defined as a deregulated host response to infection associated with organ dysfunction. Mechanisms underlying the pathophysiology of septic liver dysfunction are incompletely understood. Among others, the iron containing tetrapyrrole heme inflicts hepatic damage when relea...

journal_title：Archives of biochemistry and biophysics

authors： Englert FA,Seidel RA,Galler K,Gouveia Z,Soares MP,Neugebauer U,Clemens MG,Sponholz C,Heinemann SH,Pohnert G,Bauer M,Weis S

更新日期：2019-09-15 00:00:00

abstract：:Chloroplast tRNA(Glu) is a bifunctional molecule involved in both the early steps of chlorophyll synthesis and chloroplast protein biosynthesis. Recently the enzymes involved in these processes have been characterized from the green alga Chlamydomonas reinhardtii. In order to investigate whether transcription of the g...

journal_title：Archives of biochemistry and biophysics

authors： Jahn D

更新日期：1992-11-01 00:00:00

abstract：:Two isoforms of regulatory (R) subunit of cAMP-dependent protein kinase (PKA), named R(myt1) and R(myt2), were identified so far in the sea mussel Mytilus galloprovincialis. Out of them, only R(myt2) was phosphorylated in vitro by casein kinase 2 (CK2) using GTP as phosphate donor. CK2 catalytic subunit (CK2alpha) its...

journal_title：Archives of biochemistry and biophysics

authors： Bardales JR,Hellman U,Villamarín JA

更新日期：2007-05-01 00:00:00

abstract：:Human promatrilysin (matrix metalloproteinase-7) has been produced in Escherichia coli as an N-terminal fusion protein with ubiquitin. The insoluble product was solubilized, refolded, and activated with amino-phenylmercuric acetate. Activation of the fusion protein demonstrated kinetics and intermediates that were ver...

journal_title：Archives of biochemistry and biophysics

authors： Welch AR,Holman CM,Browner MF,Gehring MR,Kan CC,Van Wart HE

更新日期：1995-12-01 00:00:00

abstract：:Mitogens increase prostaglandin synthesis in fibroblasts. In the present studies, transcriptional activation of the prostaglandin G/H synthase isoenzyme 2 (PGHS-2) gene is shown to be responsible for this induction. Transcription of the PGHS-2 gene maximally increased within 15 min of stimulation, and elevated cellula...

journal_title：Archives of biochemistry and biophysics

authors： Evett GE,Xie W,Chipman JG,Robertson DL,Simmons DL

更新日期：1993-10-01 00:00:00

abstract：:The F1 moiety of the rat liver mitochondrial ATP synthase/ATPase complex contains as isolated 2 mol Mg2+/mol F1, 1 mol of which is nonexchangeable and the other which is exchangeable (N. Williams, J. Hullihen, and P.L. Pedersen, (1987) Biochemistry 26, 162-169). In addition, the enzyme binds 1 mol ADP/mol F1 and 3 mol...

journal_title：Archives of biochemistry and biophysics

authors： Hanley-Trawick S,Carpen ME,Dunaway-Mariano D,Pedersen PL,Hullihen J

更新日期：1989-01-01 00:00:00

abstract：:The relative reactivities of lysine residues of tropomyosin complexed with troponin have been measured in order to locate the binding site of troponin on tropomyosin in a complex between the two native proteins. The lysines were labeled with acetic anhydride using a competitive labeling procedure and the relative reac...

journal_title：Archives of biochemistry and biophysics

authors： Hitchcock-DeGregori SE,Lewis SF,Mistrik M

更新日期：1988-08-01 00:00:00

abstract：:Periodate-oxidized NADP+ (dialdehyde-NADP+) inactivated soluble ferredoxin-NADP+ oxidoreductase and combined covalently to the enzyme. This inactivation was first order with respect to dialdehyde-NADP+ and followed saturation kinetics, indicating that the enzyme initially forms a reversible complex with the inactivato...

journal_title：Archives of biochemistry and biophysics

authors： Chan RL,Carrillo N

更新日期：1984-02-15 00:00:00