Abstract：
:The effectiveness of different methodologies to identify antigenemia and antibodies against HIV at the time of viral primary infection was evaluated. To this end, 6 consecutive serum samples of a patient who had had symptomatic seroconversion were assessed. The detection of antigenemia was earlier than that of antibodies, although the sensitivity of the three evaluated assays varied greatly. An enzyme immunoassay (EIA) designed with synthetic peptides from the HIV envelope (third generation EIA) was the most sensitive test for antibody detection. Five other tests elaborated with antigenic material from a viral lysate (first generation) or with recombinant proteins (second generation) were reactive later, although the second generation EIA were more sensitive. A rapid immunofiltration test (HIV-Check) had good sensitivity, similar to that of the second generation EIA. Western blotting (WB) identified antibodies against the viral core later than the third generation EIA, and all the bands required for the diagnosis only appeared three weeks later. Therefore, although WB is an excellent method to confirm results from other tests, it may not be definitive for the results at the primary infection period when other particularly sensitive techniques are already diagnostic. We think that the higher sensitivity of EIA tests using synthetic peptides justifies its generalization for sample screening in blood banks.
journal_name
Enferm Infecc Microbiol Clinjournal_title
Enfermedades infecciosas y microbiologia clinicaauthors
Soriano V,Tor J,Ribera A,Muga R,Rivas-Mundó Msubject
Has Abstractpub_date
1990-08-01 00:00:00pages
434-7issue
7eissn
0213-005Xissn
1578-1852journal_volume
8pub_type
杂志文章abstract：:Solid organ transplant (SOT) recipients are at greater risk than the general population for complications and mortality from influenza infection. We have conducted a systematic review to assess the management and prevention of influenza infection in SOT recipients. Recommendations are provided about the procurement of...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 共识发展会议,杂志文章,实务指引,评审
doi：10.1016/j.eimc.2013.01.014
更新日期：2013-10-01 00:00:00
abstract：PURPOSE:To analyze the determinants that influence the health-related quality of life of people living with HIV in Alicante (Spain). METHODS:A cross-sectional study was conducted, which recruited 214 Spanish-speaking participants over 18 years of age living with HIV from an outpatient consulting office of the infectio...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2020.03.005
更新日期：2020-05-19 00:00:00
abstract：:An outbreak of S.Typhimurium occurred in several towns and cities in the province of Castellon (Spain) between 23 February and 27 May 2011. On April 5, the microbiology laboratory of a hospital in Castellon alerted the health authorities to the increase in S.Typhimurium isolated in fecal culture of children with gastr...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2015.11.016
更新日期：2016-11-01 00:00:00
abstract：INTRODUCTION:To describe clinical features, complications, serotypes and antibiotic resistance in pneumococcal pneumonia in our environment after the generalization of the heptavalent conjugate vaccine (PCV-7) in paediatrics. MATERIAL AND METHODS:Prospective study of episodes of pneumococcal pneumonia, with positive c...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2010.09.014
更新日期：2011-04-01 00:00:00
abstract：BACKGROUND:Plasma HIV p24 is considered a significant predictor of CD4+ T cell decline and progression to AIDS in HIV-infected patients. We evaluated the p24 levels in patients on triple therapy and after switching to ritonavir-boosted protease inhibitor monotherapy (mtPI/rtv), as well as the relationships with virolog...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2016.02.001
更新日期：2017-03-01 00:00:00
abstract：:The choice of antibiotic therapy in respiratory tract infections is usually empirical. However, this choice is complicated by the increasing prevalence of resistant strains among the major bacterial pathogens involved in these infections, particularly Streptococcus pneumoniae. The aim of antimicrobial therapy in respi...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：10.1157/13059054
更新日期：2004-04-01 00:00:00
abstract：INTRODUCTION:The aim of this investigation was to study the epidemiology of nasopharyngeal (NP) colonization with Streptococcus pneumoniae after the introduction of the heptavalent pneumococcal conjugate vaccine (PCV7). METHODS:NP swabs were obtained from 848 children aged 6 months to six years seen in four primary ca...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2011.05.010
更新日期：2011-10-01 00:00:00
abstract：:The high genetic diversity of human immunodeficiency virus (HIV) poses a significant challenge to the diagnosis and microbiological characterization of HIV infection. Because of the continual emergence of new variants and the global spread of HIV groups, subtypes and recombinant forms, accurate diagnostic tools are of...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：10.1157/13128783
更新日期：2008-11-01 00:00:00
abstract：BACKGROUND:Streptococcus agalactiae or streptococcus group B (SGB) is the main etiologic agent of early neonatal sepsis. A multicenter study was performed with the aim of determining the incidence and characteristics of this disease in our medium and contribute the design of an adequate prevention protocol. METHODS:Te...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,多中心研究
doi：
更新日期：1998-08-01 00:00:00
abstract：:Rilpivirine (RPV) is a new, second-generation nonnucleoside reverse transcriptase inhibitor (NNRTI) that has been recently approved for use in the initial antiretroviral therapy (ART) of treatment-naïve HIV-infected patients, combined with two nucleoside/nucleotide reverse transcriptase inhibitors (NRTI). The approved...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：10.1016/S0213-005X(13)70139-3
更新日期：2013-06-01 00:00:00
abstract：INTRODUCTION:St. Louis encephalitis virus (SLEV) is a re-emerging arbovirus in South America, with reported cases in humans in Argentina and Brazil. This fact indicates that there is an urgent need to increase the current knowledge about this virus in order to control and prevent future cases. Exhaustive epidemiologica...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1157/13114389
更新日期：2008-01-01 00:00:00
abstract：:Heart transplantation has emerged as the therapy of choice for selected patients with life-threatening, irremediable heart disease. Infectious complications are frequent, but are no longer the leading cause of death. Late infections (particularly viral infections), however, are still a threat to long-term graft surviv...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：10.1157/13111187
更新日期：2007-11-01 00:00:00
abstract：:Mycobacterial culture has a high sensitivity and is the test of choice for the microbiological diagnosis of tuberculosis and nontuberculous mycobacterial infections. However, the results of this culture require at least 2-3 weeks to obtain positivity. Staining is rapid and can be used as a complementary study, althoug...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2017.11.009
更新日期：2018-02-01 00:00:00
abstract：:Although the WHO original target date for the global eradication of poliomyelitis was the year 2000 -thanks to vaccination and institutional, public and private, resources for that purpose-, in 2013 the disease remained endemic in three countries, Afghanistan, Pakistan and Nigeria, and some cases were described in fiv...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：10.1016/j.eimc.2014.10.005
更新日期：2015-12-01 00:00:00
abstract：BACKGROUND:To determine the characteristics of HIV-infection in foreign patients residing in Elche, Spain. METHOD:A retrospective comparative analysis of clinical and epidemiological characteristics was performed to study HIV-infection in foreign immigrants, foreigners who were not immigrants and the native Spanish po...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1157/13078824
更新日期：2005-10-01 00:00:00
abstract：:The evolution of antimicrobial susceptibility of Bacteroides fragilis over a five year period is described. We have studied 30 selected strains isolated each year at the University Hospital of Sevilla (total: 150 strains). We did not find any resistant strain to chloramphenicol, metronidazole or imipenem. Resistance t...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:Quinolone resistance is caused mainly by chromosomal mutations in gram negative bacteria. In 1998, plasmid-mediated resistance to quinolones in clinical isolates was first reported in a Klebsiella pneumoniae strain. Locus qnr (quinolone resistance) was responsible of the quinolone resistance in this plasmid. qnr codes...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：10.1157/13070406
更新日期：2005-01-01 00:00:00
abstract：:The detection of group B streptococci by the ICON Strep-B test an enzyme immunoassay, and culture in new Granada Medium was compared in tubes and plates in a total of 200 vaginal specimens from pregnant women. The group B streptococci were cultured in new Granada medium from 33 of these specimens (incidence of 16.5%)....
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：INTRODUCTION:Trypanosoma cruzi infection is a major imported parasitic disease in Spain, because of the increase of immigrants from endemic areas. Since the laboratory diagnosis during the chronic phase is based on detection of anti-T. cruzi IgG antibodies, our aims were to compare 10 tests for determining anti-T. cruz...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2009.07.005
更新日期：2010-05-01 00:00:00
abstract：INTRODUCTION:Factors predicting short-term amputation during hospital treatment of patients admitted for acute diabetic foot infections are of interest for clinicians managing the acute episode. METHODS:A retrospective clinical records analysis of 78 consecutive patients hospitalized for acute diabetic foot infections...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2009.12.017
更新日期：2010-12-01 00:00:00
abstract：BACKGROUND:Diarrhea associated with Clostridium difficile is a health care problem of growing importance in the last few years specially in the hospital environment. The epidemiologic data and factors associated with this disease have not, to date, been sufficiently studied in Spain. METHODS:The cases of diarrhea asso...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：INTRODUCTION:The aim of this study is to describe a Shigella sonnei outbreak in a school. METHOD:An epidemiological inquiry was performed. Stool samples from 5 patients were cultured. Molecular typing of the isolates was carried out by PFGE, MLST and plasmid analysis. RESULTS:The attack rate was 14.3% (67 students). ...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1157/13100466
更新日期：2007-04-01 00:00:00
abstract：INTRODUCTION:Crimean-Congo hemorrhagic fever (CCHF) is a viral disease, mainly transmitted through tick bite, of great importance in Public Health. In Spain, Crimean-Congo hemorrhagic fever virus (CCHFV) was detected for the first time in 2010 in Hyalomma lusitanicum ticks collected from deer in Cáceres. The aim of thi...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2017.01.009
更新日期：2017-01-01 00:00:00
abstract：:This review offers succinct, precise, and complete information based on the available data concerning new anaerobic bacterial species involved in infectious diseases in humans. All hitherto undescribed species, those not previously implicated in clinical conditions, those with confirmed implication in human disease th...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：10.1016/j.eimc.2008.09.015
更新日期：2010-03-01 00:00:00
abstract：INTRODUCTION:Zika virus is mainly transmitted through the bites of infected Aedes mosquitoes, although mother-to-child and sexual transmission have also been described. The presence of Zika virus in semen after infection seems to be not uncommon, but the duration of viral persistence has not been well-determined. METH...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2016.10.009
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) has become an important hospital-acquired pathogen, with transfer of the organism from a carrier or infected patient to uninfected patients by the hands or clothing of staff as the main mode of transmission. METHODS:Investigation of a cluster of new cases o...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2012.10.002
更新日期：2013-10-01 00:00:00
abstract：:Clinical microbiology today is a well established specialty in Spain whose development has necessarily been linked to improvements in the diagnosis, treatment and prevention of infectious diseases. Over time, clinical, teaching, and research structures have been organized around these diseases. In addition, a scientif...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 历史文章,杂志文章
doi：10.1016/S0213-005X(10)70013-6
更新日期：2010-10-01 00:00:00
abstract：INTRODUCTION:Collaboration is essential for biomedical research. The Carlos III Health Institute (the Spanish national public organization responsible for promoting biomedical research) has encouraged scientific collaboration by promoting Thematic Networks and Cooperative Research Centres. Scientific collaboration in E...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2009.12.011
更新日期：2010-10-01 00:00:00
abstract：:The diffusion of hepatitis B virus was investigated in an open institution for mentally retarded patients, where 24 patients with Down's syndrome (DS) and 94 with other types of mental retardation (OMR) were being cared for. The immunogenic response to a recombinant vaccine intramuscularly injected to the deltoid musc...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：
更新日期：1990-03-01 00:00:00
abstract：INTRODUCTION:Community-onset infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are increasing. However, there is little information about community-onset bacteremia (CB) due to MRSA in Spain. The objectives of this study were to evaluate the prevalence, clinical and molecular epidemiology, clinica...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,多中心研究
doi：10.1016/j.eimc.2009.07.004
更新日期：2010-06-01 00:00:00
