Abstract：
PURPOSE:Low numbers of primary auditory neurons (ANs) may compromise the clinical performance of a cochlear implant. The focus of this research is to determine whether stem cells can be used to replace the ANs lost following deafness. To successfully replace these neurons, stem cells must be capable of directed differentiation into a sensory neural lineage in vitro and, subsequently, of survival and integration into the deafened cochlea. MATERIALS AND METHODS:In this study, we compared three in vitro treatments for directing the differentiation of mouse embryonic stem cells toward a sensory neural fate using neurotrophins, conditioned media from early post-natal cochlear epithelium, or media containing BMP4. RESULTS:In all treatments, stem cells were first exposed to retinoic acid, which was sufficient to induce Brn3a-positive patterning in 8-day differentiated embryoid bodies. After a further 8 days of differentiation in adherent culture conditions, BMP4 media-treated cultures produced higher proportions of cells expressing sensory neural markers in comparison to both the conditioned media and neurotrophin treatments, including significantly greater numbers of cells expressing peripherin (P ≤ .001), tyrosine receptor kinase B (P ≤ .001), and β-III tubulin (P ≤ .001). CONCLUSIONS:This study illustrated that combined treatment with retinoic acid and BMP4 was most effective at directing differentiation of mouse stem cells into sensory-like neurons in vitro. This finding further supports the role of bone morphogenetic proteins in the differentiation of sensory neurons from neural progenitors, and provides a basis for allotransplantation studies for auditory neuron replacement in the deaf mouse cochlea.
journal_name
Am J Otolaryngoljournal_title
American journal of otolaryngologyauthors
Nayagam BA,Minter RLdoi
10.1016/j.amjoto.2010.12.009subject
Has Abstractpub_date
2012-01-01 00:00:00pages
37-46issue
1eissn
0196-0709issn
1532-818Xpii
S0196-0709(10)00260-7journal_volume
33pub_type
杂志文章abstract：:A 37-year old man had laryngeal myxoma arising from the lingual surface of the epiglottis. Laryngeal myxoma appears to be rare, as the authors' review of the literature failed to reveal any cases reported in the past two decades. ...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(86)80036-9
更新日期：1986-01-01 00:00:00
abstract：PURPOSE:Sleep disturbances are common in patients with allergic rhinitis (AR). Perennial allergens like house dust mites (HDM) are difficult to avoid and have nocturnal impacts on the respiratory system and Quality of Life (QOL). The Rapid Eye Movement (REM) sleep stage is associated with memory, cognition, dreams, and...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102709
更新日期：2020-01-01 00:00:00
abstract：PURPOSE:Middle ear disease is increasingly being managed via transcanal endoscopic ear surgery (TEES). A limitation of TEES is that it restricts the surgeon to single-handed dissection. One solution to this would be an endoscope holder to facilitate two-handed dissection. Current endoscope holders are stationary, and c...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102875
更新日期：2020-12-29 00:00:00
abstract：OBJECTIVE:To assess the utility of rapid parathyroid hormone (PTH) values in predicting transient post-operative hypocalcemia in patients with unplanned parathyroidectomy during total or completion thyroidectomy. METHODS:All patients who underwent total or completion thyroidectomy between January 2010 and January 2015...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2019.04.006
更新日期：2019-01-01 00:00:00
abstract：PURPOSE:Many head and neck surgical procedures are considered clean-contaminated wounds and antibiotic prophylaxis is recommended. Despite prophylaxis, the incidence of surgical site infections remains significant - especially in the setting of free tissue transfer. The antibiotic course is often of a longer duration a...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2017.01.009
更新日期：2017-01-01 00:00:00
abstract：PURPOSE:Some cochlear implant (CI) patients lose their residual hearing during surgery. Two factors that might play a role in residual hearing loss are the change in intracochlear hydraulic pressure and force on the cochlear wall during electrode insertion. The aim of this study is to investigate whether a difference i...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102859
更新日期：2020-12-29 00:00:00
abstract：PURPOSE:Due to the COVID-19 pandemic, aerosol-generating procedures (AGPs) such as flexible endoscopic evaluation of swallowing (FEES) have been deemed high-risk and in some cases restricted, indicating the need for additional personal protective equipment. The aim of this study was to erect and study a protective barr...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102888
更新日期：2021-01-04 00:00:00
abstract：:Aneurysmal bone cyst is a rare lesion of the head and neck, and the mandible and maxilla are the most common sites of occurrence. To the authors' knowledge, two cases of aneurysmal bone cyst of the larynx described here are the first to be reported. Although the exact pathogenesis of this lesion is unknown, several th...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(86)80027-8
更新日期：1986-09-01 00:00:00
abstract：:Three patients with laryngeal chondrosarcoma were observed at the ENT Department of Padua University from 1966 to 1983. Including the present observations, about 150 of these neoplasms have been described in the literature. The male to female ratio was 3:1, and the age at diagnosis ranged from 33 to 91 years (median, ...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(84)80005-8
更新日期：1984-09-01 00:00:00
abstract：PURPOSE:The aim of this study was to determine the relationship of HLA typing in patients with otosclerosis. MATERIALS AND METHODS:We used a prospective study in which HLA typing of 100 surgically confirmed otosclerosis patients were compared with age and sex matched normal individuals. RESULTS:HLA-A3, HLA-A9, HLA-A1...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(99)90019-4
更新日期：1999-03-01 00:00:00
abstract：OBJECTIVE:To compare the effect of Paracetamol/Prednisolone versus Paracetamol/Ibuprofen on post-operative recovery after adult tonsillectomy. BACKGROUND:Various analgesic protocols have been proposed for the control of post-tonsillectomy morbidity with need for better control in adult population for having higher sev...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2018.05.002
更新日期：2018-01-01 00:00:00
abstract：:Phlebectasia is an abnormal dilatation of an isolated vein, may affect any vein, and is usually asymptomatic. When the internal jugular vein is involved, it presents as a cervical swelling that can mimic the signs of either pharyngocele or a laryngocele and, because of its rarity, is frequently misdiagnosed. We descri...
journal_title：American journal of otolaryngology
pub_type： 杂志文章,评审
doi：10.1016/s0196-0709(99)90085-6
更新日期：1999-11-01 00:00:00
abstract：:Cervical necrotizing fasciitis is a rapidly progressive and devastating infection that is usually caused by a polymicrobial infection including group A Streptococcus and anaerobes. We present a case of a newly diagnosed diabetic man who underwent transoral drainage of a Klebsiella pneumoniae paraglottic abscess, which...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2012.05.009
更新日期：2012-11-01 00:00:00
abstract：PURPOSE:The non-invasive diagnosis of acute rhinosinusitis (ARS) remains an unresolved problem of modern otolaryngology. Analog diaphanoscopy of reduced transillumination (shading) could be enhanced by a digital image processing of the maxillary sinuses. By this means, the limited ergonomics of this safe and low-cost m...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102444
更新日期：2020-01-01 00:00:00
abstract：:Auditory middle latency responses (latencies 6 to 50 msec in guinea pigs) were recorded from eight awake, restrained guinea pigs. Before recording, screw electrodes were implanted in the skull in a coronal plane in line with the bregma. Another electrode, which served to monitor auditory brainstem responses, was place...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(83)80013-1
更新日期：1983-03-01 00:00:00
abstract：PURPOSE:Suspension laryngoscopy is a commonly performed procedure in otolaryngology. During the procedure, the laryngoscope applies direct force to the tongue. Postoperative tongue symptoms include pain, swelling, numbness, taste disturbance, and rarely motor deficits. Duration and magnitude of force applied have previ...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102402
更新日期：2020-01-01 00:00:00
abstract：OBJECTIVE:Langerhans cell histiocytosis (LCH) is a rare clinical disorder. We retrospectively analysed the clinical manifestations, treatments and prognoses of LCH cases involving the ear, nose, and neck. MATERIALS AND METHODS:28 cases with confirmed LCH in ear, nose or neck were reviewed. We recorded patient age, sex...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2019.102369
更新日期：2020-01-01 00:00:00
abstract：INTRODUCTION:The influence on balance by the peripheral vestibular disturbance in Ménière's disease is studied. The influence of visual and proprioceptive support in balance is evaluated. MATERIALS AND METHODS:Patients with recurrent spontaneous vertigo attacks (n = 134) were studied, 90 with Ménière's disease and 44 ...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/0196-0709(93)90114-m
更新日期：1993-11-01 00:00:00
abstract：PURPOSE:Little is known about prognostic factors and treatment outcomes in young patients with anaplastic thyroid cancer (ATC). The goal of this study is to define the clinical features of this subgroup. MATERIAL AND METHODS:Patients age 55 or younger with either ATC or well-differentiated thyroid cancer (WDTC) with a...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2013.07.008
更新日期：2013-11-01 00:00:00
abstract：:The bacteriology of acute otitis media in 18 children eight to 17 years of age was investigated through the use of culture of middle ear exudate obtained by tympanic membrane puncture. Equal numbers of children had either Hemophilus influenzae (six) or Streptococcus pneumoniae (six) in the middle ear exudate. Acute ot...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/s0196-0709(81)80004-x
更新日期：1981-02-01 00:00:00
abstract：PURPOSE:This study was done to determine the direct impact implementation of the Affordable Care Act (ACA) on patients with Head and Neck Cancer (HNCA) in states that chose to expand Medicaid compared to in states that did not, as well as assess whether this impact varied among different demographic groups. MATERIALS ...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102464
更新日期：2020-01-01 00:00:00
abstract：:This article describes a woman with hemorrhagic telangiectasia, or Osler-Weber-Rendu disease, with recurrent nosebleeds despite prior septal dermoplasty and laser treatments. Telangiectasias were clearly shown at the time of angiography. Embolization of abnormal vessels on both sides resulted in cessation of epistaxis...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/0196-0709(95)90047-0
更新日期：1995-03-01 00:00:00
abstract：OBJECTIVES:We aim to present two cases of a retained insertion stylet from nasal feeding tube bridle systems. STUDY DESIGN:Case series of two patients. METHODS:Patients were evaluated from 8/2012 to 9/2013 for retained bridle system insertion stylet. RESULTS:Two patients presented with a retained stylet from nasal t...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2014.10.033
更新日期：2015-03-01 00:00:00
abstract：:Tuberculosis is known to affect almost every organ in the body, but its manifestations in the head and neck region are quite rare. A tuberculous retropharyngeal abscess is a very rare condition and can be the cause of oropharyngeal dysphagia. It is usually secondary to tuberculosis of the spine and has the potential o...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2011.06.006
更新日期：2012-03-01 00:00:00
abstract：OBJECTIVES:In the literature, diabetes mellitus was mentioned as one of the etiologic factors of olfactory disorder. However, association between olfactory dysfunction and complications of type 2 diabetes mellitus is unclear. The aim of this study was to determine if there is any correlation between olfactory dysfuncti...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2019.102365
更新日期：2020-01-01 00:00:00
abstract：OBJECTIVES:The aim of the study was to report a recent case of mucoepidermoid carcinoma (MEC) of the thyroid, review the pertinent literature, and highlight the importance of multimodal treatment in this potentially aggressive malignancy. STUDY DESIGN:The study comprise a case report and a review of literature. METHO...
journal_title：American journal of otolaryngology
pub_type： 杂志文章,评审
doi：10.1016/j.amjoto.2004.08.014
更新日期：2005-03-01 00:00:00
abstract：:The laryngeal mask airway (LMA) is regarded as a safer supraglottic airway for general anesthesia compared with conventional endotracheal tubes. We report a case of bilateral vocal cord palsy temporarily observed after using the LMA, which required urgent tracheotomy. Severe swelling of the arytenoid suggested that bi...
journal_title：American journal of otolaryngology
pub_type： 杂志文章,评审
doi：10.1016/j.amjoto.2006.07.001
更新日期：2007-03-01 00:00:00
abstract：:Opioid analgesics play an important part in the interdisciplinary management of head and neck cancer patients. Acute pain during the initial treatment period and chronic pain after treatment may be undertreated by head and neck surgeons because of unwarranted fear of the opioid side effects and toxicities. Properly us...
journal_title：American journal of otolaryngology
pub_type： 杂志文章,评审
doi：10.1053/ajot.2000.0210244
更新日期：2000-07-01 00:00:00
abstract：PURPOSE:To report our long-term results in surgical management of invasive intralabyrinthine cholesteatoma. MATERIAL AND METHODS:The study is a case series in a tertiary referral center. Retrospective chart review of all mastoid operations performed for chronic ear disease between 1994 and 2019 at University Health Ne...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2020.102407
更新日期：2020-01-01 00:00:00
abstract：OBJECTIVE:Previous research has demonstrated the safety of tracheoesophageal puncture voice prosthesis (TEP) placement in radiated patients; however, there is a growing population of twice-radiated patients with limited research on the outcomes of TEP-placement in this cohort. METHODS:After Institutional Review Board ...
journal_title：American journal of otolaryngology
pub_type： 杂志文章
doi：10.1016/j.amjoto.2019.08.003
更新日期：2019-01-01 00:00:00
