Abstract：
:Sodium dodecyl sulphate (SDS), an anionic surfactant, has been used extensively due to its low cost and excellent foaming properties. Fifteen different bacterial isolates capable of degrading SDS were isolated from detergent contaminated soil by enrichment culture technique and the degradation efficiency was assessed by Methylene Blue Active Substances (MBAS) assay. The most efficient SDS degrading isolate was selected and identified as Pseudomonas aeruginosa S7. The selected isolate was found to harbor a single 6-kb plasmid. Acridine orange, ethidium bromide, SDS and elevated temperatures of incubation failed to cure the plasmid. The cured derivatives of SDS degrading Pseudomonas aeruginosa were obtained only when ethidium bromide and elevated temperature (40 °C) were used together. Transformation of E. coli DH5α with plasmid isolated from S7 resulted in subsequent growth of the transformants on minimal salt media with SDS (0.1%) as the sole source of carbon. The SDS degradation ability of S7 and the transformant was found to be similar as assessed by Methylene Blue Active Substance Assay. The antibiotic resistance profiles of S7, competent DH5α and transformant were analyzed and it was noted that the transfer of antibiotic resistance correlated with the transfer of plasmid as well as SDS degrading property.
journal_name
Bull Environ Contam Toxicoljournal_title
Bulletin of environmental contamination and toxicologyauthors
Yeldho D,Rebello S,Jisha MSdoi
10.1007/s00128-010-0162-2subject
Has Abstractpub_date
2011-01-01 00:00:00pages
110-3issue
1eissn
0007-4861issn
1432-0800journal_volume
86pub_type
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journal_title：Bulletin of environmental contamination and toxicology
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journal_title：Bulletin of environmental contamination and toxicology
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journal_title：Bulletin of environmental contamination and toxicology
pub_type： 杂志文章
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journal_title：Bulletin of environmental contamination and toxicology
pub_type： 杂志文章
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journal_title：Bulletin of environmental contamination and toxicology
pub_type： 杂志文章,评审
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journal_title：Bulletin of environmental contamination and toxicology
pub_type： 杂志文章
doi：10.1007/s00128-012-0896-0
更新日期：2013-01-01 00:00:00
abstract：:To evaluate substance toxicity, it is critical to maintain specific concentrations of test substances throughout the exposure period. During the last decade, the need to improve methods for nanoparticle (NP) suspension preparations has gained attention because many published results on NPs toxicity have been inconsist...
journal_title：Bulletin of environmental contamination and toxicology
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doi：10.1007/s00128-014-1296-4
更新日期：2014-07-01 00:00:00
