Abstract:
:Derivatives of 4-methylumbelliferone (4MU) are favorite substrates for the measurement of lysosomal enzyme activities in a wide variety of cell and tissue specimens. Hydrolysis of these artificial substrates at acidic pH leads to the formation of 4-methylumbelliferone, which is highly fluorescent at a pH above 10. When used for the assay of enzyme activities in dried blood spots the light emission signal can be very low due to the small sample size so that the patient and control ranges are not widely separated. We have investigated the hypothesis that quenching of the fluorescence by hemoglobin leads to appreciable loss of signal and we show that the precipitation of hemoglobin with trichloroacetic acid prior to the measurement of 4-methylumbelliferone increases the height of the output signal up to eight fold. The modified method provides a clear separation of patients' and controls' ranges for ten different lysosomal enzyme assays in dried blood spots, and approaches the conventional leukocyte assays in outcome quality.
journal_name
Mol Genet Metabjournal_title
Molecular genetics and metabolismauthors
Oemardien LF,Boer AM,Ruijter GJ,van der Ploeg AT,de Klerk JB,Reuser AJ,Verheijen FWdoi
10.1016/j.ymgme.2010.09.008subject
Has Abstractpub_date
2011-01-01 00:00:00pages
44-8issue
1eissn
1096-7192issn
1096-7206pii
S1096-7192(10)00349-5journal_volume
102pub_type
杂志文章abstract::Pompe disease results from inherited deficiency of the enzyme acid alpha-glucosidase resulting in lysosomal accumulation of glycogen primarily in skeletal muscle. Reported is the first case in which a donor with late onset Pompe disease (LOPD) was successfully used for deceased donor liver and kidney transplantation. ...
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章,meta分析
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
doi:10.1016/j.ymgme.2003.10.016
更新日期:2004-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.ymgme.2011.09.004
更新日期:2011-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.ymgme.2009.02.006
更新日期:2009-06-01 00:00:00
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journal_title:Molecular genetics and metabolism
pub_type: 杂志文章
doi:10.1016/j.ymgme.2018.06.005
更新日期:2018-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.ymgme.2011.10.010
更新日期:2012-01-01 00:00:00
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journal_title:Molecular genetics and metabolism
pub_type: 杂志文章
doi:10.1006/mgme.2000.3088
更新日期:2000-11-01 00:00:00