A novel approach to processing descemet stripping automated endothelial keratoplasty specimens for histopathologic analysis.

Abstract:

PURPOSE:To describe a new technique for handling Descemet stripping automated endothelial keratoplasty (DSAEK) specimens intraoperatively and during processing. METHODS:The processing method begins by adding a drop of eosin to the specimen intraoperatively, followed by submersion in 10% formalin solution. The specimen appears reddish, allowing for easy identification and is floated onto an immersed slide to maintain an unfolded flattened specimen. After bisection, one half is stained with hematoxylin and eosin and covered with a cover slip, whereas the other half is transferred to filter paper and a cassette and is submitted for paraffin embedding on the cut edge. RESULTS:The technique reduces the chances of a specimen not surviving processing. The new method also allows for 2 views of DSAEK specimens, both topographical and cross-sectional, thus permitting analysis of endothelial cell density, spatial distribution of cells and guttae, endothelial cell count, and thickness measurements. CONCLUSIONS:A novel technique for handling DSAEK specimens to prevent loss and folding, while also allowing for cross-sectional and topographical viewing is reported.

journal_name

Cornea

journal_title

Cornea

authors

Steven Houston SK,Morgan O,Olsakovsky LA,Phillips PM,Ghazi NG

doi

10.1097/ICO.0b013e3181da57f3

subject

Has Abstract

pub_date

2010-12-01 00:00:00

pages

1452-4

issue

12

eissn

0277-3740

issn

1536-4798

journal_volume

29

pub_type

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