Abstract:
PURPOSE:To describe a new technique for handling Descemet stripping automated endothelial keratoplasty (DSAEK) specimens intraoperatively and during processing. METHODS:The processing method begins by adding a drop of eosin to the specimen intraoperatively, followed by submersion in 10% formalin solution. The specimen appears reddish, allowing for easy identification and is floated onto an immersed slide to maintain an unfolded flattened specimen. After bisection, one half is stained with hematoxylin and eosin and covered with a cover slip, whereas the other half is transferred to filter paper and a cassette and is submitted for paraffin embedding on the cut edge. RESULTS:The technique reduces the chances of a specimen not surviving processing. The new method also allows for 2 views of DSAEK specimens, both topographical and cross-sectional, thus permitting analysis of endothelial cell density, spatial distribution of cells and guttae, endothelial cell count, and thickness measurements. CONCLUSIONS:A novel technique for handling DSAEK specimens to prevent loss and folding, while also allowing for cross-sectional and topographical viewing is reported.
journal_name
Corneajournal_title
Corneaauthors
Steven Houston SK,Morgan O,Olsakovsky LA,Phillips PM,Ghazi NGdoi
10.1097/ICO.0b013e3181da57f3subject
Has Abstractpub_date
2010-12-01 00:00:00pages
1452-4issue
12eissn
0277-3740issn
1536-4798journal_volume
29pub_type
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