当前位置： SCI文献检索 > JOURNAL OF PLANT PHYSIOLOGY期刊下所有文献 > Functional characterization of a sucrose:fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance.

Functional characterization of a sucrose:fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance.

Abstract：

:We have previously reported the molecular characterization of a putative sucrose:fructan 6-fructosyltransferase (6-SFT) of Bromus pictus, a graminean species from Patagonia, tolerant to cold and drought. Here, this enzyme was functionally characterized by heterologous expression in Pichia pastoris and Nicotiana tabacum. Recombinant P. pastoris Bp6-SFT showed comparable characteristics to barley 6-SFT and an evident fructosyltransferase activity synthesizing bifurcose from sucrose and 1-kestotriose. Transgenic tobacco plants expressing Bp6-SFT, showed fructosyltransferase activity and fructan accumulation in leaves. Bp6-SFT plants exposed to freezing conditions showed a significantly lower electrolyte leakage in leaves compared to control plants, indicating less membrane damage. Concomitantly these transgenic plants resumed growth more rapidly than control ones. These results indicate that Bp6-SFT transgenic tobacco plants that accumulate fructan showed enhanced freezing tolerance compared to control plants.

journal_name

J Plant Physiol

journal_title

Journal of plant physiology

authors

Del Viso F,Casabuono AC,Couto AS,Hopp HE,Puebla AF,Heinz RA

doi

10.1016/j.jplph.2010.07.013

subject

Has Abstract

pub_date

2011-03-15 00:00:00

pages

493-9

issue

5

eissn

0176-1617

issn

1618-1328

pii

S0176-1617(10)00366-4

journal_volume

168

pub_type

杂志文章

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