Abstract:
:Assessment of biological potency and its comparison with clinical effects are important in the quality control of therapeutic glycoproteins. Animal models are usually used for evaluating bioactivity of these compounds. However, alternative methods are required to simplify the bioassay and avoid ethical issues associated with animal studies. Negatively charged sialic acid residues are known to be critical for in vivo bioactivity of recombinant human erythropoietin (rhEPO). In this study, we used capillary zone electrophoresis, a charge-based separation method, to estimate the sialic acid content for predicting in vivo bioactivity of rhEPO. In vivo bioactivities of rhEPO subfractions were measured and compared with sialylation levels. The results obtained indicated that in vivo bioactivity of rhEPO is not simply correlated with the sialylation level, which suggests that it is difficult to predict biological potency from the sialic acid content alone. N-Glycan moieties as well as sialic acid residues may have a significant impact on in vivo bioactivity of rhEPO.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Yanagihara S,Taniguchi Y,Hosono M,Yoshioka E,Ishikawa R,Shimada Y,Kadoya T,Kutsukake Kdoi
10.1248/bpb.33.1596subject
Has Abstractpub_date
2010-01-01 00:00:00pages
1596-9issue
9eissn
0918-6158issn
1347-5215pii
JST.JSTAGE/bpb/33.1596journal_volume
33pub_type
杂志文章abstract::Ferulic acid (FA), a naturally occurring polyphenol abundant in vegetables and rice bran, is known to possess a potent antioxidant activity, thereby protecting cells from oxidative stress. In the present study, we show that in addition to its known anti-oxidant activity, ferulic acid exerts substantial inhibitory acti...
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