Abstract：
:The release of [(3)H]GABA which is newly synthesized from [(3)H]l-glutamic acid (GLU) has been examined using striatal slices obtained from the rat brain. It was found that 8-10% of [(3)H]GLU transported was converted to [(3)H]GABA during the incubation of striatal slices in the presence of nipecotic acid (5 x 10(?5) M). Nipecotic acid was added to the medium in order to prevent possible reuptake of [(3)H]GABA released during its synthesis, and it was found to have no significant effect on the formation of [(3)H]GABA from [(3)H]GLU as well as on the uptake of [(3)H]GLU. The application of high potassium (60 mM) stimulation exhibited a significant enhancement of the release of this newly synthesized [(3)H]GABA in a Ca(2+) dependent manner. Kinetic analysis revealed that the evoked release of newly synthesized [(3)H]GABA was approximately two times greater than that of previously-loaded [(3)H]GABA, whereas no significant difference was observed in the spontaneous release. An immobilization stress in water failed to affect the release of newly synthesized [(3)H]GABA from striatal slices despite the occurrence of a significant enhancement of GABA formation in this structure. These results suggest that newly synthesized GABA may be preferentially released from its nerve terminals in response to the excitation of neurons at least in the striatum as compared with previously accumulated GABA.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Yoneda Y,Kanmori K,Takahashi M,Zuo PP,Paik IH,Kuriyama Kdoi
10.1016/0197-0186(84)90045-7subject
Has Abstractpub_date
1984-01-01 00:00:00pages
641-9issue
5eissn
0197-0186issn
1872-9754pii
0197-0186(84)90045-7journal_volume
6pub_type
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