Identification, characterization, and quantitation of soluble HLA antigens in the circulation and peritoneal dialysate of renal patients.

Abstract:

:Human lymphocyte antigen (HLA) class I and class II antigens and beta 2 microglobulin (B2M) were identified in peritoneal dialysate (PD) and serum from patients with end-stage renal disease (ESRD) using monoclonal antibodies in an enzyme-linked immunoassay. The HLA class I and class II antigens each exhibited approximate molecular weights of 50,000 to 60,000 daltons by chromatography on Sepharose CL 6B. Class I antigens in serum and PD fluid were associated with B2M. Free B2M (Mr 11,500) also was detected in both sera and PD fluids. Unlike class I antigens, class II antigens were not found to have attached B2M. Class I and class II antigens eluted from 2-diethylaminoethanol ion exchange gradient columns at 0.07 mol/L (molar) phosphate buffer pH 7.2 and migrated with alpha 2-beta 1 mobility in agarose electrophoresis. Class I antigens were purified from ESRD patients' PD fluid by solid-phase immunoaffinity chromatography. Enzyme-linked immunoassay demonstrated that this purified protein was composed of a class I heavy chain and B2M. Class I allospecificity was confirmed by neutralization on known HLA typing antisera in a microcytotoxicity assay. Soluble HLA class I antigen preparations specifically inhibited blast transformation of responder lymphocytes in mixed lymphocyte culture reactions. Inhibition was dose dependent and ranged from 0% to 95%. The presence of soluble HLA antigens in body fluids may play an important part in the immunologic tolerance to self. This study demonstrates a ready source of large quantities of soluble HLA for detailed analysis.

journal_name

Ann Surg

journal_title

Annals of surgery

authors

Gelder FB,McDonald JC,Landreneau MD,McMillan RM,Aultman DF

doi

10.1097/00000658-199106000-00009

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

591-7; discussion 597-9

issue

6

eissn

0003-4932

issn

1528-1140

journal_volume

213

pub_type

杂志文章
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    authors: Toyama MT,Patel AG,Nguyen T,Ashley SW,Reber HA

    更新日期:1997-02-01 00:00:00

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    abstract:OBJECTIVE:To prevent bile duct injury by using a cold 5% glucose isotonic solution cooling in the bile ducts when radiofrequency (RF) is performed in a porcine model. SUMMARY BACKGROUND DATA:Complications that may arise during liver RF ablation include biliary stenosis and abscesses. METHODS:The RITA 1500 generator w...

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    authors: Marchal F,Elias D,Rauch P,Zarnegar R,Leroux A,Stines J,Verhaeghe JL,Guillemin F,Carteaux JP,Villemot JP

    更新日期:2006-01-01 00:00:00

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    abstract:BACKGROUND:Surgical management of incidental Meckel's diverticulum(MD) is a highly debated controversial issue that has never been discussed from the oncological standpoint. OBJECTIVE:To describe the epidemiology and risk of Meckel's diverticulum cancer (MDC) and compare it with other ileal malignancies. METHODS:Data...

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    authors: Thirunavukarasu P,Sathaiah M,Sukumar S,Bartels CJ,Zeh H 3rd,Lee KK,Bartlett DL

    更新日期:2011-02-01 00:00:00

  • When Do DCD Donors Die?: Outcomes and Implications of DCD at a High-volume, Single-center OPO in the United States.

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    doi:10.1097/SLA.0000000000001298

    authors: Scalea JR,Redfield RR,Rizzari MD,Bennett R,Anderson ME,Anderson JE,Kaufman DB,Sollinger HW,Fernandez LA,D'Alessandro AM,Mezrich J

    更新日期:2016-02-01 00:00:00

  • Failure to Rescue in Emergency General Surgery: Impact of Fragmentation of Care.

    abstract:OBJECTIVE:Compare emergency general surgery (EGS) patient outcomes following index and non-index hospital readmissions, and explore predictive factors for non-index readmission. BACKGROUND:Readmission to a different hospital leads to fragmentation of care. The impact of non-index readmsision on patient outcomes follow...

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    pub_type: 杂志文章

    doi:10.1097/SLA.0000000000004628

    authors: Hanna K,Chehab M,Bible L,Asmar S,Ditillo M,Castanon L,Tang A,Joseph B

    更新日期:2020-11-17 00:00:00

  • Accelerated wound healing with stapled enteric suture lines. An experimental study comparing traditional sewing techniques and a stapling device.

    abstract::The healing of 3-cm longitudinal colotomies closed with either Czerny-Lembert suture lines (CLSL) or TA-30 staple lines (SSL) were studied in 28 mongrel dogs using bursting strength (BS) measurements. Dogs from each group were sacrificed on postoperative days (POD) 1 through 7. Mean BS for the SSL in all 14 dogs was 1...

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    pub_type: 杂志文章

    doi:10.1097/00000658-198503000-00019

    authors: Ballantyne GH,Burke JB,Rogers G,Lampert EG,Boccia J

    更新日期:1985-03-01 00:00:00

  • Intestinal radioprotection by vitamin E (alpha-tocopherol).

    abstract:OBJECTIVES:The major objective of this study was to test vitamin E as a potential radioprotectant for the small bowel of the rat. SUMMARY BACKGROUND DATA:Vitamin E has previously been shown to provide radioprotection in animal models: increased survival after whole-body irradiation, diminished absorptive malfunction, ...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-199522240-00008

    authors: Felemovicius I,Bonsack ME,Baptista ML,Delaney JP

    更新日期:1995-10-01 00:00:00

  • Metabolic consequences of (regional) total pancreatectomy.

    abstract::Little information has been reported on the metabolic characteristics of the totally pancreatectomized patient or the efficacy of medical management after radical pancreatic surgery. The prospective evaluation of 49 such patients, with 31% followed for 48 or more months, forms the basis of this report. The major immed...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-199108000-00007

    authors: Dresler CM,Fortner JG,McDermott K,Bajorunas DR

    更新日期:1991-08-01 00:00:00

  • Management of spontaneous umbilical hernia disruption in the cirrhotic patient.

    abstract::Umbilical hernia is a common finding in cirrhotic patients with ascites. Spontaneous disruption of the hernia and attendant discharge of ascitic fluid is an unusual and rarely reported complication in these patients and is associated with an overall mortality rate of nearly 30%. During the 5-year period 1977-1982, nin...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-198307000-00006

    authors: Lemmer JH,Strodel WE,Knol JA,Eckhauser FE

    更新日期:1983-07-01 00:00:00

  • Influence of pentobarbital and chloralose on metabolic and hemodynamic changes in liver ischemia.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-199007000-00004

    authors: Nordström G,Winsö O,Biber B,Hasselgren PO

    更新日期:1990-07-01 00:00:00

  • The impact of immunostimulating nutrition on infectious complications after upper gastrointestinal surgery: a prospective, randomized, clinical trial.

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    pub_type: 杂志文章,随机对照试验

    doi:10.1097/SLA.0b013e318180a3c1

    authors: Klek S,Kulig J,Sierzega M,Szybinski P,Szczepanek K,Kubisz A,Kowalczyk T,Gach T,Pach R,Szczepanik AM

    更新日期:2008-08-01 00:00:00

  • Thoracoscopic surgery for diseases of the lung and pleura. Effectiveness, changing indications, and limitations.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-199305010-00018

    authors: Daniel TM,Kern JA,Tribble CG,Kron IL,Spotnitz WB,Rodgers BM

    更新日期:1993-05-01 00:00:00

  • Total pancreatectomy with preservation of the duodenum and pylorus for chronic pancreatitis.

    abstract::In an effort to minimize the nutritional complications that follow resection of the pancreas for severe chronic pancreatitis, the authors have performed a duodenum-preserving total pancreatectomy in eight patients for severe unremitting pain requiring large doses of opiate analgesia. Good relief of pain was obtained i...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-199111000-00007

    authors: Easter DW,Cuschieri A

    更新日期:1991-11-01 00:00:00

  • A model for predicting the risk of carotid artery disease.

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    pub_type: 杂志文章

    doi:10.1097/SLA.0b013e31827b9761

    authors: Greco G,Egorova NN,Moskowitz AJ,Gelijns AC,Kent KC,Manganaro AJ,Zwolak RM,Riles TS

    更新日期:2013-06-01 00:00:00

  • Maintenance of cardiodynamics with aspirin during abdominal aortic aneurysmectomy (AAA).

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    journal_title:Annals of surgery

    pub_type: 临床试验,杂志文章

    doi:10.1097/00000658-198111000-00008

    authors: Utsunomiya T,Krausz MM,Dunham B,Mannick JA,Allen PD,Shepro D,Hechtman HB

    更新日期:1981-11-01 00:00:00

  • Can CT-PET and Endoscopic Assessment Post-Neoadjuvant Chemoradiotherapy Predict Residual Disease in Esophageal Cancer?

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    journal_title:Annals of surgery

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    更新日期:2016-11-01 00:00:00

  • Primary Open Versus Closed Implantation Strategy for Totally Implantable Venous Access Ports: The Multicentre Randomized Controlled PORTAS-3 Trial (DRKS 00004900).

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    pub_type: 杂志文章

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  • Clinical significance of the mesorectal extension of rectal cancer: a Japanese multi-institutional study.

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    更新日期:2011-04-01 00:00:00

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    pub_type: 杂志文章

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    pub_type: 杂志文章

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