A new methodology to obtain wine yeast strains overproducing mannoproteins.

Abstract:

:Yeast mannoproteins are highly glycosylated proteins that are covalently bound to the beta-1,3-glucan present in the yeast cell wall. Among their outstanding enological properties, yeast mannoproteins contribute to several aspects of wine quality by protecting against protein haze, reducing astringency, retaining aroma compounds and stimulating growth of lactic-acid bacteria. The development of a non-recombinant method to obtain enological yeast strains overproducing mannoproteins would therefore be very useful. Our previous experience on the genetic determinants of the release of these molecules by Saccharomyces cerevisiae has allowed us to propose a new methodology to isolate and characterize wine yeast that overproduce mannoproteins. The described methodology is based on the resistance of the killer 9 toxin produced by Williopsis saturnus, a feature linked to an altered biogenesis of the yeast cell wall.

journal_name

Int J Food Microbiol

authors

Quirós M,Gonzalez-Ramos D,Tabera L,Gonzalez R

doi

10.1016/j.ijfoodmicro.2010.02.014

subject

Has Abstract

pub_date

2010-04-30 00:00:00

pages

9-14

issue

1-2

eissn

0168-1605

issn

1879-3460

pii

S0168-1605(10)00095-4

journal_volume

139

pub_type

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