Abstract:
:Yeast mannoproteins are highly glycosylated proteins that are covalently bound to the beta-1,3-glucan present in the yeast cell wall. Among their outstanding enological properties, yeast mannoproteins contribute to several aspects of wine quality by protecting against protein haze, reducing astringency, retaining aroma compounds and stimulating growth of lactic-acid bacteria. The development of a non-recombinant method to obtain enological yeast strains overproducing mannoproteins would therefore be very useful. Our previous experience on the genetic determinants of the release of these molecules by Saccharomyces cerevisiae has allowed us to propose a new methodology to isolate and characterize wine yeast that overproduce mannoproteins. The described methodology is based on the resistance of the killer 9 toxin produced by Williopsis saturnus, a feature linked to an altered biogenesis of the yeast cell wall.
journal_name
Int J Food Microbioljournal_title
International journal of food microbiologyauthors
Quirós M,Gonzalez-Ramos D,Tabera L,Gonzalez Rdoi
10.1016/j.ijfoodmicro.2010.02.014subject
Has Abstractpub_date
2010-04-30 00:00:00pages
9-14issue
1-2eissn
0168-1605issn
1879-3460pii
S0168-1605(10)00095-4journal_volume
139pub_type
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