Intracellular calcium elevation induced by extracellular application of cyclic-ADP-ribose or oxytocin is temperature-sensitive in rodent NG108-15 neuronal cells with or without exogenous expression of human oxytocin receptors.


:ADP-ribosyl cyclase and/or CD38 are activated after oxytocin receptor stimulation in the hypothalamus and pituitary in adult mice, leading to facilitation of oxytocin secretion. Although cyclic adenosine 5'-diphosphoribose (cADPR) primarily acts as an intracellular second messenger, it has been suggested that extracellular cADPR stimulates intracellular ryanodine receptors after internalisation via the nucleotide-transporting capacity of CD38 in fibroblasts and astrocytes. However, little is known about whether extracellular cADPR activates neurones. To address this question, we used a model neuronal cell line, NG108-15 mouse neuroblastoma x rat glioma hybrid cells possessing CD38 but not oxytocin receptors, and measured cytosolic free calcium concentrations ([Ca(2+)](i)). Extracellular application of cADPR to NG108-15 cells elevated [Ca(2+)](i) at 35 degrees C. The elevation was significantly enhanced when measured at 40 degrees C. The cADPR and heat-induced [Ca(2+)](i) increase were blocked under extracellular Ca(2+)-free conditions and by 2-aminoethoxydiphenyl borate, an antagonist of melastatin-related transient receptor potential channel 2 (TRPM2) cation channels. Reverse transcriptation-polymerase chain reaction analyses indicated that TRPM2 channels were expressed in NG108-15 cells. Application of oxytocin elevated [Ca(2+)](i) in NG108-15 cells transformed to transiently express cloned human oxytocin receptors. The oxytocin-induced [Ca(2+)](i) response was also enhanced by heat. These results indicate that the extracellular application of cADPR, together with heat, activates cation influx downstream of oxytocin receptor signalling in NG108-15 neuronal cells, and suggest the possible involvement of TRPM2 channels in oxytocin release in the mammalian brain.


J Neuroendocrinol


Amina S,Hashii M,Ma WJ,Yokoyama S,Lopatina O,Liu HX,Islam MS,Higashida H




Has Abstract


2010-05-01 00:00:00














  • Nucleobindin 2/nesfatin-1 expression and colocalisation with neuropeptide Y and cocaine- and amphetamine-regulated transcript in the human brainstem.

    abstract::Feeding is a complex behaviour entailing elaborate interactions between forebrain, hypothalamic and brainstem neuronal circuits via multiple orexigenic and anorexigenic neuropeptides. Nucleobindin-2 (NUCB2)/nesfatin-1 is a negative regulator of food intake and body weight with a widespread distribution in rodent brain...

    journal_title:Journal of neuroendocrinology

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    authors: Psilopanagioti A,Makrygianni M,Nikou S,Logotheti S,Papadaki H

    更新日期:2020-09-01 00:00:00

  • GnRH mRNA increases with puberty in the male Syrian hamster brain.

    abstract::Puberty in the male Syrian hamster (Mesocricetus auratus) is characterized by decreased responsiveness to testosterone mediated negative feedback, but the neural mechanism for this change remains elusive. We hypothesized that decreased inhibition of the gonadotropin-releasing hormone (GnRH) system results in increased...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: Parfitt DB,Thompson RC,Richardson HN,Romeo RD,Sisk CL

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  • Altered ratios of beta-endorphin: beta-lipotropin released from anterior lobe corticotropes with increased secretory drive. I. Effects of diminished glucocorticoid secretion.

    abstract::Previous studies have demonstrated that acute stress or ovine corticotropin-releasing hormone (oCRH) in vivo, or oCRH in vitro, stimulates release of beta-endorphin over beta-lipotropin from anterior pituitary corticotropes. This occurs despite the predominance of beta-lipotropin in corticotrope peptide stores. In vit...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: Young EA,Przewlocki R,Patel P,Watson SJ,Akil H

    更新日期:1993-02-01 00:00:00

  • Non-adrenocorticotropin mediated effects of endogenous corticotropin-releasing factor on the adrenocortical activity in the rat.

    abstract::Diurnal changes in plasma corticosterone concentrations of rats are supposed to be controlled by low amplitude changes in plasma adrenocorticotropin (ACTH) that run in phase with marked changes in adrenal sensitivity to ACTH (1, 2). These sensitivity changes are probably under neuronal control (3), but the mechanisms ...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: Oers JW,Tilders FJ

    更新日期:1991-04-01 00:00:00

  • Elevated corticosterone levels during the first postpartum period influence subsequent pregnancy outcomes and behaviours of the dam.

    abstract::Postpartum depression affects 15% of new mothers and previous depressive episodes increase the risk for postpartum depression. Chronic administration of corticosterone (CORT) to dams during the postpartum period causes depressive-like behaviour and has been used as a model of postpartum depression. To better understan...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: Wong JH,Brummelte S,Galea LA

    更新日期:2011-11-01 00:00:00

  • Oestrogen requires the insulin-like growth factor-I receptor for stimulation of prolactin synthesis via mitogen-activated protein kinase.

    abstract::Sex steroids and growth factors interact at the intracellular level in a variety of tissues to control numerous physiological functions. Oestrogen is known to stimulate prolactin synthesis and secretion, but the effect of insulin-like growth factor (IGF)-I is less clear. We used GH3 cells, a somatolactotroph cell line...

    journal_title:Journal of neuroendocrinology

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    authors: Arroba AI,Frago LM,Argente J,Chowen JA

    更新日期:2005-02-01 00:00:00

  • Hypothalamic galanin-like peptide rescues the onset of puberty in food-restricted weanling rats.

    abstract::Galanin-like peptide (GALP) is a known mediator of metabolism and reproduction; however, the role that GALP plays in the onset of puberty is unknown. First, we tested the hypothesis that central GALP administration could rescue puberty in food-restricted weanling rats. GALP treatment in food-restricted rats of both se...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: Mohr MA,Leathley E,Fraley GS

    更新日期:2012-11-01 00:00:00

  • Oestrogen, cognition and the maturing female brain.

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    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章,评审


    authors: Craig MC,Murphy DG

    更新日期:2007-01-01 00:00:00

  • Glucocorticoid enhances the neurotoxic actions of quinolinic acid in the striatum in a cell-specific manner.

    abstract::This study demonstrates that corticosterone can exacerbate the damaging effects of infused quinolinic acid (QA) on the dorsal striatum. Adult adrenalectomised male rats were pretreated subcutaneously with graded doses of corticosterone (0, 0.5, 2, 5, 20 and 40 mg/kg/day) for 2 days and then received a unilateral infus...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: Ngai LY,Herbert J

    更新日期:2005-07-01 00:00:00

  • Timing of thyroid hormone action in the developing brain: clinical observations and experimental findings.

    abstract::Abstract The original concept of the critical period of thyroid hormone (TH) action on brain development was proposed to identify the postnatal period during which TH supplement must be provided to a child with congenital hypothyroidism to prevent mental retardation. As neuropsychological tools have become more sensit...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章,评审


    authors: Zoeller RT,Rovet J

    更新日期:2004-10-01 00:00:00

  • Pro-Opiomelanocortin (POMC) Neurones, POMC-Derived Peptides, Melanocortin Receptors and Obesity: How Understanding of this System has Changed Over the Last Decade.

    abstract::Following the cloning of the melanocortin receptor and agouti protein genes, a model was developed for the central melanocortin system with respect to the regulation of energy and glucose homeostasis. This model comprised leptin regulation of melanocortin peptides and agouti-related peptide (AgRP) produced from centra...

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    authors: Mountjoy KG

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    abstract::Abstract The possibility that steroids exert their effects on luteinizing hormone (LH) and prolactin release and female sexual behaviour via altering gamma-aminobutyric acid (GABA) synthesis within the hypothalamus was investigated. Ovariectomized rats were treated with either oil, 5 mug oestradiol benzoate (OB) or 5 ...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: Leigh AJ,Carter ND,Horton R,Silverligh JJ,Wilson CA

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  • Analysis of steroid-induced genes in the rat preoptic area-anterior hypothalamus using a differential-display reverse transcriptase-polymerase chain reaction.

    abstract::Steroid hormones modulate a variety of physiological functions in the hypothalamus. We attempted to identify steroid-regulated genes in the rat preoptic area-anterior hypothalamus by comparing differentially expressed mRNAs. Adult female rats were ovariectomized and, 1 week later, a silastic capsule containing 17beta-...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: Park S,Seong JY,Son GH,Kang SS,Lee S,Kim SR,Kim K

    更新日期:2001-06-01 00:00:00

  • A subset of estrogen receptor-containing neurons project to the median eminence in the ewe.

    abstract::The neural pathways responsible for conveying the steroid feedback signals that ultimately affect reproductive neuroendocrine function remain largely undefined. One possibility involves a direct projection from estrogen receptor (ER)-containing neurons to the median eminence (ME), a site of neuroendocrine peptide rele...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: Jansen HT,Hileman SM,Lubbers LS,Jackson GL,Lehman MN

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  • Reproduction-induced neuroplasticity: natural behavioural and neuronal alterations associated with the production and care of offspring.

    abstract::As a female transitions into motherhood, many neurobiological adaptations are required to meet the demands presented by her offspring. In addition to the traditional maternal responses (e.g. crouching, nursing, retrieving, grooming), our laboratories have observed several behavioural modifications accompanying parity,...

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    pub_type: 杂志文章,评审


    authors: Kinsley CH,Lambert KG

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  • Effect of short-and long-term gonadectomy on neuroactive steroid levels in the central and peripheral nervous system of male and female rats.

    abstract::Significant levels of neuroactive steroids are still detected in the nervous system of rodents after the removal of peripheral steroidogenic glands. However, the influence of the plasma levels of gonadal steroids on the levels of neuroactive steroids in the nervous system has not so far been clarified in detail. Accor...

    journal_title:Journal of neuroendocrinology

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    authors: Caruso D,Pesaresi M,Maschi O,Giatti S,Garcia-Segura LM,Melcangi RC

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  • From sensory circumventricular organs to cerebral cortex: Neural pathways controlling thirst and hunger.

    abstract::Much progress has been made during the past 30 years with respect to elucidating the neural and endocrine pathways by which bodily needs for water and energy are brought to conscious awareness through the generation of thirst and hunger. One way that circulating hormones influence thirst and hunger is by acting on neu...

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    authors: McKinley MJ,Denton DA,Ryan PJ,Yao ST,Stefanidis A,Oldfield BJ

    更新日期:2019-03-01 00:00:00

  • Acute ghrelin changes food preference from a high-fat diet to chow during binge-like eating in rodents.

    abstract::Ghrelin, an orexigenic hormone released from the empty stomach, provides a gut-brain signal that promotes many appetitive behaviours, including anticipatory and goal-directed behaviours for palatable treats high in sugar and/or fat. In the present study, we aimed to determine whether ghrelin is able to influence and/o...

    journal_title:Journal of neuroendocrinology

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    authors: Bake T,Hellgren KT,Dickson SL

    更新日期:2017-04-01 00:00:00

  • Gonadotropin-releasing hormone exhibits circadian rhythm in phase with arginine-vasopressin in co-cultures of the female rat preoptic area and suprachiasmatic nucleus.

    abstract::To determine whether the suprachiasmatic nucleus can drive a circadian release of gonadotropin-releasing hormone (GnRH) in the preoptic area, we measured the release of GnRH, arginine-vasopressin and vasoactive intestinal polypeptide (VIP) in cocultures of the preoptic area and the suprachiasmatic nucleus at 2-h inter...

    journal_title:Journal of neuroendocrinology

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    authors: Funabashi T,Shinohara K,Mitsushima D,Kimura F

    更新日期:2000-06-01 00:00:00

  • Hypothalamic-pituitary-adrenal function in chronic intermittently cold-stressed neonatally handled and non handled rats.

    abstract::Neonatally handled (H) animals, as adults, exhibit lower ACTH and corticosterone (B) responses to a number of acute stressors compared to their non-handled (NH) counterparts. However, little is known about activity within the hypothalamic-pituitary-adrenal (HPA) axis of H and NH animals under conditions of chronic str...

    journal_title:Journal of neuroendocrinology

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    authors: Bhatnagar S,Meaney MJ

    更新日期:1995-02-01 00:00:00

  • Y5 receptor signalling counteracts the anorectic effects of PYY3-36 in diet-induced obese mice.

    abstract::Peptide YY 3-36 (PYY3-36) is known as a critical satiety factor that reduces food intake both in rodents and humans. Although the anorexic effect of PYY3-36 is assumed to be mediated mainly by the Y2 receptor, the involvement of other Y-receptors in this process has never been conclusively resolved. Amongst them, the ...

    journal_title:Journal of neuroendocrinology

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    authors: Shi YC,Ip CK,Reed F,Sarruf DA,Wulff BS,Herzog H

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  • Mechanisms mediating oestradiol modulation of the developing brain.

    abstract::The brain has been known to be a sensitive target organ for the permanent organisational effects of gonadal steroids for close to 50 years. Recent advances have revealed a variety of unexpected cellular mechanisms by which steroids impact on the synaptic profile of hypothalamic nuclei critical to the control of reprod...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章,评审


    authors: McCarthy MM,Schwarz JM,Wright CL,Dean SL

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  • Encoding time of day and time of year by the avian circadian system.

    abstract::The most important zeitgeber for seasonal rhythmicity of physiology and behaviour in birds is the annual cycle of photoperiod. Regulatory mechanisms are less well understood in birds than in mammals since photic information can be perceived by photoreceptors in the retina and the pineal gland, as well as in the brain,...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章,评审


    authors: Brandstätter R

    更新日期:2003-04-01 00:00:00

  • Variations in maternal behaviour are associated with differences in oxytocin receptor levels in the rat.

    abstract::Female Long-Evans rats exhibit stable individual differences in maternal behaviours such as pup licking/grooming and arched-back nursing posture (LG-ABN). These variations in maternal behaviour are accompanied by differences in lactation-induced increases in oxytocin receptor levels in brain regions known to mediate t...

    journal_title:Journal of neuroendocrinology

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    authors: Francis DD,Champagne FC,Meaney MJ

    更新日期:2000-12-01 00:00:00

  • Neuronal Uptake of Monoclonal Anti-Vasopressin Antibodies in vivo and Relationship with the Physiological Status of Vasopressin Neurosecretory Cells.

    abstract::Abstract Mouse monoclonal anti-vasopressin antibody was injected just above one hypothalamic paraventricular nucleus of the rat brain. Immunocytochemistry and morphometric analysis showed that the antibody was taken up by neurons with the size and stereotaxic distribution of vasopressin-producing neurons. Labelled neu...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: Burlet A,Arahmani A,Tankosic P,Burlet C

    更新日期:1990-06-01 00:00:00

  • Distribution of aromatase mRNA in the ram hypothalamus: an in situ hybridization study.

    abstract::The regional distribution of neurones expressing aromatase mRNA in the ram hypothalamus was examined by in situ hybridization using 33P-labelled cRNA probes. The highest amounts of hybridization signal were observed in the central part of the medial preoptic nucleus and posterior medial part of the bed nucleus of the ...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: Roselli CE,Stormshak F,Resko JA

    更新日期:2000-07-01 00:00:00

  • APJ receptor mRNA expression in the rat hypothalamic paraventricular nucleus: regulation by stress and glucocorticoids.

    abstract::The apelin receptor (APJ receptor, APJR) has recently come to prominence following the isolation and identification of its endogenous ligand, apelin, from bovine stomach tissue extracts. Investigation of APJR mRNA expression has revealed a hypothalamic distribution similar to that of vasopressin suggesting that the ap...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: O'Carroll AM,Don AL,Lolait SJ

    更新日期:2003-11-01 00:00:00

  • Simultaneous Visualization of Vasopressin and Oxytocin mRNA- Containing Neurons in the Hypothalamus Using Non-Radioactive in situ Hybridization Histochemistry.

    abstract::In situ hybridization histochemistry (ISHH) using synthetic oligodeoxynucleotide probes has been used to demonstrate the sites of expression of mRNA for vasopressin (AVP) and oxytocin (OXT) in the rat brain. ISHH was performed with two types of non-radioactive probes, labelled with either alkaline phosphatase or 5'-bi...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: Ichimiya Y,Emson PC,Christodoulou C,Gait MJ,Ruth JL

    更新日期:1989-04-01 00:00:00

  • A functional promoter directing expression of a novel type of rat mineralocorticoid receptor mRNA in brain.

    abstract::The authors have identified two types of hippocampal cDNAs for the rat mineralocorticoid receptor (rMR) which are identical in the protein coding domain but differ in their 5'-untranslated sequences. One of these clones encodes a novel type of rMR cDNA with a high homology to a previously described human MR cDNA isola...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: Castrén M,Damm K

    更新日期:1993-08-01 00:00:00

  • Growth hormone-releasing hormone (GHRH)-GH-somatic growth and luteinizing hormone (LH)RH-LH-ovarian axes in adult female transgenic mice expressing human GH gene.

    abstract::We have examined alterations in the hypothalamo-pituitary GH-somatic growth axis and the hypothalamo-pituitary LH-ovarian axis in a line of transgenic ICR mice expressing human GH (hGH) under the influence of the whey acid protein promoter. Transgenic female mice weighed twice as much as control females and were infer...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章


    authors: Sasaki F,Tojo H,Iwama Y,Miki N,Maeda K,Ono M,Kiso Y,Okada T,Matsumoto Y,Tachi C

    更新日期:1997-08-01 00:00:00