当前位置： SCI文献检索 > ONCOGENE期刊下所有文献 > The MYCN protein of human neuroblastoma cells is phosphorylated by casein kinase II in the central region and at serine 367.

The MYCN protein of human neuroblastoma cells is phosphorylated by casein kinase II in the central region and at serine 367.

Abstract：

:The MYCN gene has been implicated in certain neuronal tumours, such as neuroblastomas and retinoblastomas. These tumours express high levels of mRNA and protein of MYCN as a result of amplification. MYCN encodes a short-lived nuclear phosphoprotein whose function has not yet been elucidated. This study was undertaken to determine the pattern of MYCN protein (pMYCN) phosphorylation in human neuroblastoma cells. We report that pMYCN is phosphorylated in vitro by purified casein kinase II (CK-II). Two-dimensional phosphopeptide maps showed that most of the phosphopeptides of pMYCN phosphorylated in vitro by CK-II correspond to those phosphorylated in vivo. Fine mapping of the phosphorylation sites was performed using two synthetic MYCN peptides corresponding to pMYCN CK-II consensus sequences. Both peptides were found to be phosphorylated by CK-II and competitively inhibited CK-II phosphorylation of pMYCN in vitro. Thus, we have localized two major CK-II phosphorylation sites in pMYCN, one to the highly acidic central region and the second to serine 367 proximal to the C-terminus. Our data demonstrate that pMYCN is a physiological substrate for CK-II and, since CK-II activity is stimulated in response to mitogens, CK-II phosphorylation of pMYCN may, therefore, represent one signal transduction pathway used by neuroblastoma cells.

journal_name

Oncogene

journal_title

Oncogene

authors

Hamann U,Wenzel A,Frank R,Schwab M

subject

Has Abstract

pub_date

1991-10-01 00:00:00

pages

1745-51

issue

10

eissn

0950-9232

issn

1476-5594

journal_volume

6

pub_type

杂志文章

相关文献

ONCOGENE文献大全
  • Tyrosine phosphorylation and complex formation of Cbl-b upon T cell receptor stimulation.

    abstract：:Cbl-b, a mammalian homolog of Cbl, consists of an N-terminal region (Cbl-b-N) highly homologous to oncogenic v-Cbl, a Ring finger, and a C-terminal region containing multiple proline-rich stretches and potential tyrosine phosphorylation sites. In the present study, we demonstrate that upon engagement of the T cell rec...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/sj.onc.1202411

    authors： Elly C,Witte S,Zhang Z,Rosnet O,Lipkowitz S,Altman A,Liu YC

    更新日期：1999-02-04 00:00:00

  • The chicken Pdcd4 gene is regulated by v-Myb.

    abstract：:The retroviral oncogene v-myb encodes a transcription factor (v-Myb) which is responsible for the ability of avian myeloblastosis virus (AMV) to transform myelomonocytic cells. v-Myb is thought to disrupt the differentiation of myelomonocytic cells by affecting the expression of specific target genes. To identify such...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/sj.onc.1204071

    authors： Schlichter U,Burk O,Worpenberg S,Klempnauer KH

    更新日期：2001-01-11 00:00:00

  • Discovery of HTLV-1, the first human retrovirus, its unique regulatory mechanisms, and insights into pathogenesis.

    abstract：:I briefly review the discovery and characterization of the first human retrovirus, human T-cell leukemia virus type 1, focusing on contributions from Japanese researchers. The unique regulatory mechanisms for the viral regulation with Tax and Rex, etiology of ATL and possible leukemogenic mechanism with Tax are also d...

    journal_title：Oncogene

    pub_type： 历史文章,杂志文章,评审

    doi：10.1038/sj.onc.1208981

    authors： Yoshida M

    更新日期：2005-09-05 00:00:00

  • p53 in complex with DNA is resistant to ubiquitin-dependent proteolysis in the presence of HPV-16 E6.

    abstract：:The tumour suppressor p53 is a transcription factor with high affinity for specific DNA target sequences. Wild type p53 has a very short half life in normal cells but the protein shows transient accumulation in response to DNA damage, accompanied by up-regulation of target genes such as p21 and induction of growth arr...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：

    authors： Molinari M,Milner J

    更新日期：1995-05-04 00:00:00

  • Autocrine activation of JAK2 by IL-11 promotes platinum drug resistance.

    abstract：:Antineoplastic platinum agents are used in first-line treatment of ovarian cancer, but treatment failure frequently results from platinum drug resistance. Emerging observations suggest a role of reactive oxygen species (ROS) in the resistance of cancer drugs including platinum drugs. However, the molecular link betwee...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/s41388-018-0238-8

    authors： Zhou W,Sun W,Yung MMH,Dai S,Cai Y,Chen CW,Meng Y,Lee JB,Braisted JC,Xu Y,Southall NT,Shinn P,Huang X,Song Z,Chen X,Kai Y,Cai X,Li Z,Hao Q,Cheung ANY,Ngan HYS,Liu SS,Barak S,Hao J,Dai Z,Tzatsos A,Peng W

    更新日期：2018-07-01 00:00:00

  • Roles of the Pas1 and Par2 genes in determination of the unique, intermediate susceptibility of BALB/cByJ mice to urethane-induction of lung carcinogenesis: differential effects on tumor multiplicity, size and Kras2 mutations.

    abstract：:The C3H/HeJ (C3H), A/J and BALB/cByJ (BALB) mouse strains are respectively resistant, sensitive and intermediate regarding the induction of lung tumors by urethane. The phenotypic difference between C3H and A/J is largely determined by the Pas1 (Pulmonary adenoma susceptibility 1) gene on chromosome 6, the A/J allele ...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/sj.onc.1201357

    authors： Karasaki H,Obata M,Ogawa K,Lee GH

    更新日期：1997-10-09 00:00:00

  • Crosstalk between NRF2 and HIPK2 shapes cytoprotective responses.

    abstract：:Homeodomain interacting protein kinase-2 (HIPK2) is a member of the HIPK family of stress-responsive kinases that modulates cell growth, apoptosis, proliferation and development. HIPK2 has several well-characterised tumour suppressor roles, but recent studies suggest it can also contribute to tumour progression, altho...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/onc.2017.221

    authors： Torrente L,Sanchez C,Moreno R,Chowdhry S,Cabello P,Isono K,Koseki H,Honda T,Hayes JD,Dinkova-Kostova AT,de la Vega L

    更新日期：2017-11-02 00:00:00

  • Adaptive upregulation of FOXD3 and resistance to PLX4032/4720-induced cell death in mutant B-RAF melanoma cells.

    abstract：:Melanoma cells driven by mutant v-raf murine sarcoma viral oncogene homolog B1 (B-RAF) are highly resistant to chemotherapeutic treatments. Recent phase 1 results with PLX4032/RG7204/vemurafenib, which selectively inhibits B-RAF/mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK)1...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/onc.2011.424

    authors： Basile KJ,Abel EV,Aplin AE

    更新日期：2012-05-10 00:00:00

  • TXNIP potentiates Redd1-induced mTOR suppression through stabilization of Redd1.

    abstract：:The mammalian target of rapamycin (mTOR) is a highly conserved serine-threonine kinase activated in response to growth factors and nutrients. Because of frequent dysregulation of the mTOR signaling pathway in diverse human cancers, this kinase is a key therapeutic target. Redd1 is a negative regulator of mTOR, mediati...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/onc.2011.102

    authors： Jin HO,Seo SK,Kim YS,Woo SH,Lee KH,Yi JY,Lee SJ,Choe TB,Lee JH,An S,Hong SI,Park IC

    更新日期：2011-09-01 00:00:00

  • Transcription factors Oct-1 and NF-YA regulate the p53-independent induction of the GADD45 following DNA damage.

    abstract：:The p53-regulated GADD45 gene is one of the important players in cellular response to DNA damage, and probably involved in the control of cell cycle checkpoint, apoptosis and DNA repair. There are both the p53-dependent and -independent pathways that regulate GADD45 induction. Following ionizing radiation, induction o...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/sj.onc.1204390

    authors： Jin S,Fan F,Fan W,Zhao H,Tong T,Blanck P,Alomo I,Rajasekaran B,Zhan Q

    更新日期：2001-05-10 00:00:00

  • Downregulation of c-fos gene transcription in cells transformed by E1A and cHa-ras oncogenes: a role of sustained activation of MAP/ERK kinase cascade and of inactive chromatin structure at c-fos promoter.

    abstract：:REF cells transformed by oncogenes E1A and cHa-ras reveal high and constitutive DNA-binding activity of AP-1 factor lacking in c-Fos protein. Consistently, the transcription of c-fos gene has been found to be downregulated. To elucidate the mechanisms of c-fos downregulation in E1A+cHa-ras transformants, we studied th...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/sj.onc.1205118

    authors： Kukushkin AN,Abramova MV,Svetlikova SB,Darieva ZA,Pospelova TV,Pospelov VA

    更新日期：2002-01-24 00:00:00

  • Targeting AU-rich element-mediated mRNA decay with a truncated active form of the zinc-finger protein TIS11b/BRF1 impairs major hallmarks of mammary tumorigenesis.

    abstract：:Altered expression of regulatory RNA-binding proteins (RBPs) in cancer leads to abnormal expression of mRNAs encoding many factors involved in cancer hallmarks. While conventional anticancer therapies usually target one pathway at a time, targeting key RBP would affect multiple genes and thus overcome drug resistance....

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/s41388-019-0784-8

    authors： Rataj F,Planel S,Denis J,Roelants C,Filhol O,Guyon L,Feige JJ,Cherradi N

    更新日期：2019-06-01 00:00:00

  • Human T-cell leukemia virus type I and adult T-cell leukemia.

    abstract：:Human T-cell leukemia virus type I (HTLV-I) causes adult T-cell leukemia (ATL) in about 5% of carriers after a long latent period. After its infection, HTLV-I promotes the clonal proliferation of HTLV-I infected cells in vivo by actions of encoded viral proteins, including Tax. However, leukemic cells frequently lack ...

    journal_title：Oncogene

    pub_type： 杂志文章,评审

    doi：10.1038/sj.onc.1206551

    authors： Matsuoka M

    更新日期：2003-08-11 00:00:00

  • Alterations in the p16(INK4a) and p53 tumor suppressor genes of hTERT-immortalized human fibroblasts.

    abstract：:Exogenous expression of the catalytic subunit of telomerase, hTERT, in a normal human foreskin fibroblast cell strain resulted in telomerase activity and an extended proliferative lifespan prior to a period of crisis. Three immortalized cell lines with stably maintained telomere lengths were established from cells tha...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/sj.onc.1207440

    authors： Noble JR,Zhong ZH,Neumann AA,Melki JR,Clark SJ,Reddel RR

    更新日期：2004-04-15 00:00:00

  • A member of the ras gene superfamily is expressed specifically in T, B and myeloid hemopoietic cells.

    abstract：:A 1.26 kb murine cDNA having 31% homology with human ras and 55% homology with human rho proteins was isolated using an oligonucleotide probe homologous to catalytic subdomain of a tyrosine kinase subfamily. Northern blot analysis indicates that the expression of the murine gene is restricted to the cells of hemopoiet...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：

    authors： Shirsat NV,Pignolo RJ,Kreider BL,Rovera G

    更新日期：1990-05-01 00:00:00

  • Akt regulates nuclear/cytoplasmic localization of tuberin.

    abstract：:The autosomal dominantly inherited disease tuberous sclerosis (TSC) affects approximately 1 in 6000 individuals and is characterized by the development of tumors, named hamartomas, in different organs. TSC1, encoding hamartin, and TSC2, encoding tuberin are tumor suppressor genes responsible for TSC. Hamartin and tube...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/sj.onc.1209812

    authors： Rosner M,Freilinger A,Hengstschläger M

    更新日期：2007-01-25 00:00:00

  • Hepatitis B virus X protein induces the expression of MTA1 and HDAC1, which enhances hypoxia signaling in hepatocellular carcinoma cells.

    abstract：:Expression level of metastasis-associated protein 1 (MTA1) is closely related to tumor growth and metastasis in various cancers. Although increased expression level of MTA1 was observed in hepatocellular carcinoma (HCC), role of MTA1 complex containing histone deacetylase (HDAC) in hepatitis B virus (HBV)-associated h...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/sj.onc.1211000

    authors： Yoo YG,Na TY,Seo HW,Seong JK,Park CK,Shin YK,Lee MO

    更新日期：2008-05-29 00:00:00

  • Matrix metalloproteinase processing of PTHrP yields a selective regulator of osteogenesis, PTHrP1-17.

    abstract：:Parathyroid hormone-related protein (PTHrP) is a critical regulator of bone resorption and augments osteolysis in skeletal malignancies. Here we report that the mature PTHrP1-36 hormone is processed by matrix metalloproteinases to yield a stable product, PTHrP1-17. PTHrP1-17 retains the ability to signal through PTH1R...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/onc.2017.70

    authors： Frieling JS,Shay G,Izumi V,Aherne ST,Saul RG,Budzevich M,Koomen J,Lynch CC

    更新日期：2017-08-01 00:00:00

  • Ras adenoviruses modulate cyclin E protein expression and DNA synthesis after partial hepatectomy.

    abstract：:Ras-genes encode for proteins important for transmitting extracellular signals from the cytoplasm to the nucleus. In this study we investigated the impact of Ras on cell cycle progression after hepatectomy by using adenoviral vectors (adv) expressing beta-galactosidase (beta-gal), a dominant-negative (Ras N17) or a do...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/sj.onc.1204690

    authors： Lüdde T,Kubicka S,Plümpe J,Liedtke C,Manns MP,Trautwein C

    更新日期：2001-08-30 00:00:00

  • The neural guidance receptor Plexin C1 delays melanoma progression.

    abstract：:Plexin C1 is a type I transmembrane receptor with intrinsic R-Ras GTPase activity, which regulates cytoskeletal remodeling and adhesion in normal human melanocytes. Melanocytes are pigment-producing cells of the epidermis, precursors for melanoma, and express high levels of Plexin C1, which is lost in melanoma in vitr...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/onc.2012.511

    authors： Chen Y,Soong J,Mohanty S,Xu L,Scott G

    更新日期：2013-10-10 00:00:00

  • An E mu-BCL-2 transgene facilitates leukaemogenesis by ionizing radiation.

    abstract：:Clonogenic murine B cell precursors are normally ultrasensitive to apoptosis following genotoxic exposure in vitro but can be protected by expression of an E mu-BCL-2 transgene. Such exposures are likely to be mutagenic. This in turn suggests that a level of in vivo genotoxic exposure that usually has minimal patholog...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/sj.onc.1202721

    authors： Gibbons DL,MacDonald D,McCarthy KP,Cleary HJ,Plumb M,Wright EG,Greaves MF

    更新日期：1999-07-01 00:00:00

  • Androgen regulation of soluble guanylyl cyclasealpha1 mediates prostate cancer cell proliferation.

    abstract：:The growth and progression of prostate cancer are dependent on androgens and androgen receptor (AR), which act by modulating gene expression. Utilizing a gene microarray approach, we have identified the alpha1-subunit gene of soluble guanylyl cyclase (sGC) as a novel androgen-regulated gene. A heterodimeric cytoplasmi...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/sj.onc.1209956

    authors： Cai C,Chen SY,Zheng Z,Omwancha J,Lin MF,Balk SP,Shemshedini L

    更新日期：2007-03-08 00:00:00

  • PKA signaling drives mammary tumorigenesis through Src.

    abstract：:Protein kinase A (PKA) hyperactivation causes hereditary endocrine neoplasias; however, its role in sporadic epithelial cancers is unknown. Here, we show that heightened PKA activity in the mammary epithelium generates tumors. Mammary-restricted biallelic ablation of Prkar1a, which encodes for the critical type-I PKA ...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/onc.2014.41

    authors： Beristain AG,Molyneux SD,Joshi PA,Pomroy NC,Di Grappa MA,Chang MC,Kirschner LS,Privé GG,Pujana MA,Khokha R

    更新日期：2015-02-26 00:00:00

  • KDM8/JMJD5 as a dual coactivator of AR and PKM2 integrates AR/EZH2 network and tumor metabolism in CRPC.

    abstract：:During the evolution into castration or therapy resistance, prostate cancer cells reprogram the androgen responses to cope with the diminishing level of androgens, and undergo metabolic adaption to the nutritionally deprived and hypoxia conditions. AR (androgen receptor) and PKM2 (pyruvate kinase M2) have key roles in...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/s41388-018-0414-x

    authors： Wang HJ,Pochampalli M,Wang LY,Zou JX,Li PS,Hsu SC,Wang BJ,Huang SH,Yang P,Yang JC,Chu CY,Hsieh CL,Sung SY,Li CF,Tepper CG,Ann DK,Gao AC,Evans CP,Izumiya Y,Chuu CP,Wang WC,Chen HW,Kung HJ

    更新日期：2019-01-01 00:00:00

  • Transcriptional and post-transcriptional induction of the TGFalpha gene in transformed rat liver epithelial cells.

    abstract：:Although TGFalpha mRNA and protein are frequently elevated in neoplastic cells, neither the level at which deregulation occurs nor the mechanism(s) responsible have been well characterized. As a first step, we examined the induction of TGFalpha mRNA in two series of clonally-derived rat liver epithelial cell lines tha...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：

    authors： Berkowitz EA,Hissong MA,Lee DC

    更新日期：1996-05-02 00:00:00

  • p185neu is phosphorylated on tyrosine in human primary breast tumors which overexpress neu/erbB-2.

    abstract：:A series of primary human breast tumors was analysed by immunoblotting with anti-neu antibodies. Overproduction of the neu protein-tyrosine kinase, p185neu, was observed in 23 of 56 malignant tumor samples. 29 of these tumors were also immunoblotted with antiphosphotyrosine antibodies. A single prominent phosphotyrosi...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：

    authors： Wildenhain Y,Pawson T,Blackstein ME,Andrulis IL

    更新日期：1990-06-01 00:00:00

  • WAVE3, an actin-polymerization gene, is truncated and inactivated as a result of a constitutional t(1;13)(q21;q12) chromosome translocation in a patient with ganglioneuroblastoma.

    abstract：:Neuroblastoma (Nb) is a malignancy of the sympathetic nervous system which affects children in their first decade. It is the most common extra-cranial solid tumor in children with an incidence of approximately 1 in 8-10 000 live births annually and accounts for approximately 10% of all children's cancers. Ganglioneuro...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/sj.onc.1205734

    authors： Sossey-Alaoui K,Su G,Malaj E,Roe B,Cowell JK

    更新日期：2002-08-29 00:00:00

  • Etoposide and cisplatin induced apoptosis in activated RAW 264.7 macrophages is attenuated by cAMP-induced gene expression.

    abstract：:Previous observations suggest expression of cyclooxygenase-2 to convey macrophage protection towards apoptotic cell death. We reasoned prostaglandin formation and in turn a cAMP increase as the underlying protective principle. Here we report that exposure of macrophages to lipopolysaccharide/interferon-gamma or lipoph...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/sj.onc.1201926

    authors： von Knethen A,Lotero A,Brüne B

    更新日期：1998-07-23 00:00:00

  • PCAF is an HIF-1alpha cofactor that regulates p53 transcriptional activity in hypoxia.

    abstract：:The p53 tumour suppressor is involved in several crucial cellular functions including cell-cycle arrest and apoptosis. p53 stabilization occurs under hypoxic and DNA damage conditions. However, only in the latter scenario is stabilized p53 capable of inducing the expression of its pro-apoptotic targets. Here we presen...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/onc.2008.192

    authors： Xenaki G,Ontikatze T,Rajendran R,Stratford IJ,Dive C,Krstic-Demonacos M,Demonacos C

    更新日期：2008-10-02 00:00:00

  • BRCA1 and GADD45 mediated G2/M cell cycle arrest in response to antimicrotubule agents.

    abstract：:BRCA1 is a tumour suppressor gene implicated in the predisposition to early onset breast and ovarian cancer. We have generated cell lines with inducible expression of BRCA1 to evaluate its role in mediating the cellular response to various chemotherapeutic drugs commonly used in the treatment of breast and ovarian can...

    journal_title：Oncogene

    pub_type： 杂志文章

    doi：10.1038/sj.onc.1204712

    authors： Mullan PB,Quinn JE,Gilmore PM,McWilliams S,Andrews H,Gervin C,McCabe N,McKenna S,White P,Song YH,Maheswaran S,Liu E,Haber DA,Johnston PG,Harkin DP

    更新日期：2001-09-27 00:00:00