Abstract:
:CYP1A1 and CYP1A2 enzymes metabolize polycyclic aromatic hydrocarbons (PAHs) to the reactive oxyderivatives. PAHs can induce the activity of both enzymes, which increases its conversion and enhances risk of carcinogenesis. Thus, the inhibition of CYP enzymes is recognized as a cancer chemoprevention strategy. A well-known group of chemopreventive agents is isothiocyanates, which occur naturally in Brassica vegetables. In this paper, a naturally occurring sulforaphane and its two synthetic analogues isothiocyanate-2-oxohexyl and alyssin were investigated. The aim of the study was to determine whether the differences in the isothiocyanate structure change its ability to inhibit CYP1A1 and CYP1A2 activity induced by benzo[a]pyrene in HepG2 and Mcf7 cells. Also a mechanistic study was performed including isothiocyanates' influence on CYP1A1 and CYP1A2 catalytic activity, enzymatic protein level, and AhR translocation. It was shown that both enzymes were significantly induced by benzo[a]pyrene, and isothiocyanates were capable of decreasing the induced activity. The inhibitory properties depend on the types of isothiocyanate and enzyme. In general, CYP1A2 was altered in the more meaningful way than CYP1A1 by isothiocyanates. Sulforaphane exhibited weak inhibitory properties, whereas both analogues were capable of inhibiting BaP-induced activity with the similar efficacy. The mechanistic study revealed that analogues decreased the CYP1A2 activity via the protein-level reduction and CYP1A1 directly. The results indicate that isothiocyanates can be considered as potent chemopreventive substances and the change in the sulforaphane structure increases its chemopreventive potency.
journal_name
J Biochem Mol Toxicoljournal_title
Journal of biochemical and molecular toxicologyauthors
Skupinska K,Misiewicz-Krzeminska I,Stypulkowski R,Lubelska K,Kasprzycka-Guttman Tdoi
10.1002/jbt.20259subject
Has Abstractpub_date
2009-01-01 00:00:00pages
18-28issue
1eissn
1095-6670issn
1099-0461journal_volume
23pub_type
杂志文章abstract::Oral squamous cell carcinoma (OSCC) is the most common malignant neoplasm with high rate of lymph node metastasis and recurrence. The aim of this study is to evaluate the expression profile of T Lymphoma Invasion and Metastasis 1 (TIAM1) in OSCC and its correlation with clinical outcomes including lymph node metastasi...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21875
更新日期:2017-04-01 00:00:00
abstract::This study was aimed to predict bisphenol-A (BPA)-responsive miRNA's using an in silico approach and to study their expression in granulosa cells of animals exposed prenatally to BPA. Pregnant Wistar rats were exposed to BPA through water (25 μg/L, 250 μg/L, and 2.5 mg/L) during gestation. The expression of miRNA-133b...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22317
更新日期:2019-06-01 00:00:00
abstract::The effects of ellagic acid (EA) and vitamin E succinate (VES) on 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced oxidative stress in different brain regions of rats have been studied after subchronic exposure to the compounds. TCDD was administered to groups of rats at a dose of 46 ng/kg/day for 90 days. EA and VE...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20030
更新日期:2004-01-01 00:00:00
abstract::We investigated the effects of acute diazepam (DZP) administration on thiobarbituric acid-reactive substance (TBARS) levels, protein carbonyl content, and on the activities of the antioxidant enzymes catalase, glutathione peroxidase, and superoxide dismutase in the brain of rats. Additionally, we investigated the anti...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21815
更新日期:2016-10-01 00:00:00
abstract::The action of carbenoxolone on hepatic energy metabolism was investigated in the perfused rat liver and isolated mitochondria. In perfused livers, carbenoxolone (200-300 microM) increased oxygen consumption, glucose production and glycolysis from endogenous glycogen. Gluconeogenesis from lactate or fructose, an energy...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20139
更新日期:2006-01-01 00:00:00
abstract::We have analyzed gene expression and histopathology of rat liver treated with a histamine-3 receptor inverse agonist under development for the treatment of obesity 24 h after a single acute administration. While histopathology did not identify a clear liver toxicity, analysis of gene changes strongly suggested the dev...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20375
更新日期:2011-05-01 00:00:00
abstract::Chrysin (CH) or 5,7-dihydroxyflavone is a flavonoid present in various plants, bee propolis, and honey. Cyclophosphamide (CYP) is a chemotherapeutic drug, which is extensively used in the treatment of multiple human malignancies. In our study, we aimed to investigate the effects of CYP and CH on some metabolic enzymes...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22313
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND:Cadmium is a nonessential toxic heavy metal, which enters the body easily and damages the cellular system. The sonic hedgehog (Shh) signaling pathway is one of the key regulatory pathways, which define neural growth and development. OBJECTIVES:This study aimed to explore how cadmium exposure affects neural ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22274
更新日期:2019-04-01 00:00:00
abstract::In the present study, we have tested the beneficial effects of forskolin in protecting the mancozeb-induced reproductive toxicity in rats. Adult male Wistar rats were exposed to either mancozeb (500 mg/kg body weight/day) or forskolin (5 mg/kg body weight/day) or both for 65 days and analyzed for spermatogenesis and s...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22026
更新日期:2018-02-01 00:00:00
abstract::Aluminum and thallium may reach life-threatening levels in aquatic systems in the near future because of their extensive use in various industrial fields. It is therefore important to study the mechanism of toxicity of aluminum and thallium on fish enzymes. To this aim, the effects of aluminum and thallium on the acti...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20200
更新日期:2007-01-01 00:00:00
abstract::The oxysterol 7beta-hydroxycholesterol (7beta-OH) has been shown to induce apoptosis in a number of cell lines. Though not fully elucidated, the mechanism through which this oxysterol induces cell death is thought to involve the generation of an oxidative stress leading to perturbation of the mitochondrion and release...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20007
更新日期:2004-01-01 00:00:00
abstract::The widespread opinion that N(2)O(3) as a product of NO oxidation is the only nitros(yl)ating agent under aerobic conditions is based on experiments in homogeneous buffered water solutions. In vivo NO is oxidized in heterogeneous media and this opinion is not correct. The equilibrium in the system being dependent on t...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章,评审
doi:10.1002/jbt.10029
更新日期:2002-01-01 00:00:00
abstract::Oxidative shock and production of reactive oxygen species are known to play a major role in situations leading to neuron degeneration, but the precise mechanisms responsible for cell degeneration remain uncertain. In the present article, we have studied in PC 12 cells the effect of cumene hydroxyperoxide on both cell ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20377
更新日期:2011-07-01 00:00:00
abstract::The present study is the first attempt to report the characterization of a nucleotidase from Cerastes cerastes venom. A 70 kDa 5'-nucleotidase (Cc-5'NTase) was purified to homogeneity. The amino acid sequence of Cc-5'NTase displayed high homology with many nucleotidases. Its activity was optimal at pH 7 with a specifi...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21885
更新日期:2017-05-01 00:00:00
abstract::Diepoxybutane (DEB) is the most potent active metabolite of the environmental chemical 1,3-butadiene (BD). BD is a known mutagen and human carcinogen and possesses multisystems organ toxicity. We previously reported the elevation of p53 in human TK6 lymphoblasts undergoing DEB-induced apoptosis. In this study, we have...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20300
更新日期:2009-11-01 00:00:00
abstract::Minimizing the adverse effects of chemotherapeutic agents remains a therapeutic challenge. Cisplatin (CP) induces hepatotoxicity through activation of oxidative stress, inflammation, and apoptosis cascades which is considered a significant drawback. Thus, this study aimed to highlight the possible hepatoprotective rol...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22714
更新日期:2021-01-25 00:00:00
abstract::Schisandrin A (SchA) has been reported as a multidrug resistance-reversing agent; however, its antitumor effects have been rarely reported. Consequently, we attempted to explore whether SchA per se possesses an antitumor property in choriocarcinoma JEG-3 and BeWo cells and its potential mechanisms. JEG-3, BeWo, and HT...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22455
更新日期:2020-04-01 00:00:00
abstract::Cisplatin is a highly cytotoxic anticarcinogenic drug used to treat several kinds of solid tumors such as liver tumors. With the increase in the incidences associated with hepatic tumors and a lack of selectivity of cisplatin to cancer cells, it is important to explore new therapeutic strategies against them. The pres...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22722
更新日期:2021-01-23 00:00:00
abstract::Paraoxon, the active metabolite of parathion, can be detoxified through a noncatalytic pathway by carboxylesterases and a catalytic pathway by calcium-dependent A-esterases, producing p-nitrophenol as a common metabolite. The detoxication patterns of carboxylesterases and A-esterases were investigated in vitro in the ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/(sici)1099-0461(1999)13:5<261::aid-jbt6>3.
更新日期:1999-01-01 00:00:00
abstract::Certain ginsenosides, also known as triterpene glycosides, have been recently reported to have a characteristic effect on cultured intestinal and leukemia cell growth. Ginsenoside aglycones 20(S)-protopanaxadiol (PD), 20(S)-protopanaxatriol (PT), and ginsenoside Rh2 have been identified as having a strong effect on re...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20019
更新日期:2004-01-01 00:00:00
abstract::Endotoxin exposure elicits various responses in mammals including the acute phase response that has been shown to cause changes in the activity of several forms of cytochrome P450s and other enzymes. Therefore, the hepatic conjugating enzyme, glutathione S-transferase (GST), and UDP-glucuronosyltransferase (UDPGT), th...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/(sici)1099-0461(1999)13:2<63::aid-jbt1>3.0
更新日期:1999-01-01 00:00:00
abstract::We studied action of inorganic phosphate (P(i)) on toxic effects of Tl+ in isolated rat liver mitochondria. This is a convenient model to study the toxicity of heavy metals. P(i) markedly retarded contraction of energized mitochondria swollen in the TlNO3 medium and even stronger stimulated swelling and state 4 of suc...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20215
更新日期:2008-05-01 00:00:00
abstract::We reported earlier in a guinea pig model that exposure of 2-chloroethyl ethyl sulfide (CEES), a mustard gas analog, causes lung injury associated with the activation of tumor necrosis factor alpha (TNF-alpha), mitogen activated protein kinases (MAPK) signaling, and activator protein-1 (AP-1) transcription factor. Our...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.20329
更新日期:2010-05-01 00:00:00
abstract::Excessive exposure to Copper (Cu) may result in Cu toxicity and adversely affect health outcomes. We investigated the protective role of rutin on Cu-induced brain damage. Experimental rats were treated as follows: group I: control; group II: Cu-sulfate: 200 mg/kg; group III: Cu-sulfate, and rutin 100 mg/kg; and group ...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22623
更新日期:2021-01-01 00:00:00
abstract::Allyl alcohol hepatotoxicity is mediated by an alcohol dehydrogenase-derived biotranformation product, acrolein. This highly reactive alpha,beta-unsaturated aldehyde readily alkylates model proteins in vitro, forming, among other products, Michael addition adducts that possess a free carbonyl group. Whether such damag...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.10007
更新日期:2001-01-01 00:00:00
abstract::Zingerone (ZO), an active phenolic agent derived from Zingiber officinale (Ginger), has many pharmacological properties such as antioxidant, antiangiogenic, and antitumor. However, its potential value in cancer and the mechanism by which ZO wields its therapeutic effects remain obscure. Therefore, in this current stud...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22403
更新日期:2019-12-01 00:00:00
abstract::Atrazin is currently the most widely used herbicide in agriculture with lots of adverse effects on human health. Curcumin is a polyphenol known for its antioxidant, anti-inflammatory, and anticancer properties. In the present study, the protective effect of curcumin on atrazin-intoxicated rats is evaluated. Toxicity w...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.21574
更新日期:2014-08-01 00:00:00
abstract::Colorectal cancer (CRC) is the leading type of diagnosed cancer; globally, it resides in the fourth-leading origin of cancer-interrelated mortality in the globe. The treatment strategies were chemotherapy and potent radiotherapy. Although chemotherapy treatment can eliminate tumor cells, it remains with unnecessary to...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22665
更新日期:2020-12-28 00:00:00
abstract::Retinoic acid (RA) alters the developmental fate of the axial skeletal anlagen. "Anteriorizations" or "posteriorizations," the assumption of characteristics of embryonic areas normally anterior or posterior to the affected tissues, are correlated with altered embryonal expression domains of Hox genes after in utero RA...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.10098
更新日期:2003-01-01 00:00:00
abstract::In this study, a series of novel bis-sulfone compounds (2a-2j) were synthesized by oxidation of the bis-sulfides under mild reaction conditions. The bis-sulfone derivatives were characterized by 1 H-NMR, 13 C-NMR, Fourier-transform infrared spectroscopy, and elemental analysis techniques. Nuclear Overhauser effect exp...
journal_title:Journal of biochemical and molecular toxicology
pub_type: 杂志文章
doi:10.1002/jbt.22401
更新日期:2019-11-01 00:00:00