Abstract:
OBJECTIVES:To investigate the contribution of a real-time PCR assay for the detection of Treponema pallidum in various biological specimens with the secondary objective of comparing its value according to HIV status. METHODS:Prospective cohort of incident syphilis cases from three Swiss hospitals (Geneva and Bern University Hospitals, Outpatient Clinic for Dermatology of Triemli, Zurich) diagnosed between January 2006 and September 2008. A case-control study was nested into the cohort. Biological specimens (blood, lesion swab or urine) were taken at diagnosis (as clinical information) and analysed by real-time PCR using the T pallidum 47 kDa gene. RESULTS:126 specimens were collected from 74 patients with primary (n = 26), secondary (n = 40) and latent (n = 8) syphilis. Among primary syphilis, sensitivity was 80% in lesion swabs, 28% in whole blood, 55% in serum and 29% in urine, whereas among secondary syphilis, it was 20%, 36%, 47% and 44%, respectively. Among secondary syphilis, plasma and cerebrospinal fluid were also tested and provided a sensitivity of 100% and 50%, respectively. The global sensitivity of T pallidum by PCR (irrespective of the compartment tested) was 65% during primary, 53% during secondary and null during latent syphilis. No difference regarding serology or PCR results was observed among HIV-infected patients. Specificity was 100%. CONCLUSIONS:Syphilis PCR provides better sensitivity in lesion swabs from primary syphilis and displays only moderate sensitivity in blood from primary and secondary syphilis. HIV status did not modify the internal validity of PCR for the diagnosis of primary or secondary syphilis.
journal_name
Sex Transm Infectjournal_title
Sexually transmitted infectionsauthors
Gayet-Ageron A,Ninet B,Toutous-Trellu L,Lautenschlager S,Furrer H,Piguet V,Schrenzel J,Hirschel Bdoi
10.1136/sti.2008.034314subject
Has Abstractpub_date
2009-08-01 00:00:00pages
264-9issue
4eissn
1368-4973issn
1472-3263pii
sti.2008.034314journal_volume
85pub_type
杂志文章,多中心研究abstract:OBJECTIVE:To examine sources of repeat Chlamydia trachomatis infections using behavioural and molecular methods. METHODS:Women with C. trachomatis had baseline and 4-month follow-up visits consisting of behavioural surveys and genotyping of C. trachomatis. Frequencies and population-attributable risk percentages (PAR%...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2010.045484
更新日期:2011-04-01 00:00:00
abstract:BACKGROUND/OBJECTIVES:The HIV prevalence in Eastern Europe and Central Asia continues to increase. While injection drug use (IDU) is leading factor, heterosexual transmission is on the rise. Little is known about female sex workers (FSWs) in the region despite the central role of commercial sex in heterosexual sexually...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2011-050171
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND:Vulvo-vaginal swabs (VVSs) are not validated for use by the manufacturers of two widely used nucleic acid amplification tests (NAAT) for the detection of Chlamydia trachomatis. However, there is evidence that this type of swab is suitable for diagnosis. OBJECTIVE:To validate the Cobas Taqman CT assay for th...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sti.2007.029587
更新日期:2008-08-01 00:00:00
abstract:OBJECTIVES:In addition to researcher-designed sampling biases, population-representative surveys for biomarker measurement of STIs often have substantial missingness due to non-contact, non-consent and other study-implementation issues. STI prevalence estimates may be biased if this missingness is related to STI risk. ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2019-054342
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVES:HIV/AIDS risk is embodied within multiple levels including structural and social levels. The aim of this study was to assess the effects of neighbourhood characteristics on HIV prevalence among injection drug users (IDU) residing in the area of Tallinn, Estonia in 2007. METHODS:A cross-sectional, multilevel...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2009.040212
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVE:To pilot and evaluate sexually transmitted infection (STI) management in community family planning clinics (FPCs). METHODS:Number of STI tests taken, positive results, infections treated, contacts traced/treated, referrals to specialist services and time from testing to treatment were documented as well as a...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2003.004382
更新日期:2004-04-01 00:00:00
abstract:OBJECTIVES:A new molecular test for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) (GeneXpert CT/NG) has been demonstrated to be as accurate as conventional nucleic acid amplification tests (NAAT), but performance has not been evaluated in routine primary care, performed at the point of care by clinicians. W...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,随机对照试验
doi:10.1136/sextrans-2017-053443
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:To determine the association between oral contraceptive and condom use, and laboratory-confirmed sexually transmitted infection (STI) among African-American adolescent females at a high risk of STI acquisition. METHODS:A cross-sectional study of 715 African-American adolescent females (15-21 years old) was c...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2006.022442
更新日期:2007-06-01 00:00:00
abstract:OBJECTIVES:Concurrency is suggested as an important factor in sexually transmitted infection transmission and acquisition, though little is known regarding factors that may predict concurrency initiation. We examined the association between perception of a partner's non-monogamy (PPNM) and simultaneous or subsequent co...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2015-052111
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVE:There is an urgent need to understand high HIV-infection rates among young women in sub-Saharan Africa. While age-disparate partnerships have been characterised with high-risk sexual behaviours, the mechanisms through which these partnerships may increase HIV-risk are not fully understood. This study assessed...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2018-053577
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVE:To develop an analytical understanding of non-cohabiting sex partnering in sub-Saharan Africa (SSA) using nationally representative sexual behaviour data. METHOD:A non-homogenous Poisson stochastic process model was used to describe the dynamics of non-cohabiting sex. The model was applied to 25 countries in...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2014-051925
更新日期:2015-09-01 00:00:00
abstract:OBJECTIVES:To describe sexual risk behaviour, correlates of unprotected anal intercourse (UAI) and never testing for HIV and its implications for HIV prevention interventions among men who have sex with men (MSM) in Nigeria and other similar contexts. METHODS:A cross-sectional survey was administered to 712 MSM in Abu...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sextrans-2013-051065
更新日期:2013-12-01 00:00:00
abstract:OBJECTIVE:To evaluate the impact of Mycoplasma genitalium on the outcome of pregnancy. METHODS:Cervical samples from women who had previously participated in a case-control study (designed to assess the impact of syphilis and HIV-2 on the outcome of pregnancy in Guinea-Bissau) were processed using a PCR assay to detec...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.78.4.289
更新日期:2002-08-01 00:00:00
abstract:OBJECTIVES:To explore the experiences and views of men who have sex with men (MSM) on attending clinical sexual health services and their preferences regarding service characteristics in the context of the disproportionate burden of STIs experienced by this group. The wider study aim was to develop a risk assessment to...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sextrans-2016-052983
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVES:We evaluated the performance of an enzymatic point-of-care rapid test for Chlamydia trachomatis (CT) (the BioChekSwab CT Rapid Test, EnZtek Diagnostics, Rio Vista, California, USA), which detects CT's Peptidase 123CBV enzyme and provides a result 15 min after specimen collection. METHODS:Two endocervical sw...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2015-052202
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVES:To compare two methods for sampling female sex workers (FSWs) for bio-behavioural surveillance. We compared the populations of sex workers recruited by the venue-based Priorities for Local AIDS Control Efforts (PLACE) method and a concurrently implemented network-based sampling method, respondent-driven samp...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2012-050638
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVES:Although the prevalence of sexually transmitted infections (STIs) among girls infected with HIV has been reported, the incidence of STI diagnoses has not been well documented. The objectives of this study were to examine (1) incident STI diagnoses and (2) the association between viral load (VL) and incident ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2007.026161
更新日期:2007-10-01 00:00:00
abstract:OBJECTIVES:Trichomoniasis (TV) is associated with an increased risk of acquisition of sexually transmitted diseases (STDs) and HIV. The purpose of this study is to evaluate factors associated with incidence TV among female STD clinic attendees in the USA. METHODS:Data were collected from women participating in a rando...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1136/sextrans-2015-052010
更新日期:2016-02-01 00:00:00
abstract:OBJECTIVES:To compare the characteristics of a self-selected, convenience sample of men who have sex with men (MSM) recruited through the internet with MSM drawn from a national probability survey in Great Britain. METHODS:The internet sample (n = 2065) was recruited through two popular websites for homosexual men in ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2006.023283
更新日期:2007-06-01 00:00:00
abstract:OBJECTIVES:To (i) describe the contraceptive practices of HIV care and treatment (HCTx) clients in Manzini, Swaziland, including their unmet needs for family planning (FP), and compare these with population-level estimates; and (ii) qualitatively explore the causal factors influencing contraceptive choice and use. MET...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2013-051339
更新日期:2014-08-01 00:00:00
abstract:OBJECTIVES:To assess changes in seroadaptive behaviours among men who have sex with men (MSM) in San Francisco over the past 4 years. METHODS:461 MSM were recruited in 2008 as the second wave of the US National HIV Behavioural Surveillance (NHBS) survey in San Francisco. Participants were classified into patterns of s...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2010.042986
更新日期:2011-03-01 00:00:00
abstract::We aimed to study the prevalence, characteristics and risk factors of asymptomatic sexually transmitted infections (STIs) in HIV-infected men who have sex with men (MSM). We conducted a prospective cross-sectional study, including asymptomatic HIV-infected MSM attending regular visits between December 2014 and Decembe...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2020-054560
更新日期:2020-08-04 00:00:00
abstract:OBJECTIVES:To analyse changes in testing for sexually transmissible infections (STI) among gay and bisexual men in Melbourne, Sydney and Queensland, Australia, particularly comprehensive STI testing (at least four tests from different anatomical sites in the previous year), and the characteristics of men who had such t...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sextrans-2013-051294
更新日期:2014-05-01 00:00:00
abstract:OBJECTIVE:To investigate the characteristics of South African men who have sex with men (MSM) who (1) have been tested for HIV and (2) are HIV positive. METHODS:Data were collected from 1045 MSM in community surveys using questionnaires that were administered either face-to-face, by mail or on the internet. The mean a...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sti.2008.031500
更新日期:2008-11-01 00:00:00
abstract:OBJECTIVES:To study the tolerance and acceptability in Africa of a perinatal intervention to prevent vertical HIV transmission using benzalkonium chloride disinfection. DESIGN:A randomized, double blinded phase II trial. SETTING:Prenatal care units in Abidjan (Côte d'Ivoire) and Bobo-Dioulasso (Burkina Faso). PATIEN...
journal_title:Sexually transmitted infections
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1136/sti.75.6.420
更新日期:1999-12-01 00:00:00
abstract:OBJECTIVES:To describe the mode of presentation, interventions, and outcome of HIV infected patients with pulmonary embolism and a contraindication to anticoagulation, who were treated with a bird's nest filter. METHODS:Retrospective review of case records and imaging department database at UCL Hospitals, London, UK. ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.76.5.395
更新日期:2000-10-01 00:00:00
abstract:OBJECTIVES:The objectives of this study were to assess the prevalence of ciprofloxacin-resistant gonorrhoea in two South African cities and to investigate the association between the isolation of ciprofloxacin-resistant Neisseria gonorrhoeae and the HIV serostatus of patients. METHODS:Gonococci were cultured from endo...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sti.2007.029611
更新日期:2008-10-01 00:00:00
abstract:OBJECTIVES:Ethnic differences in partnership types and sexual mixing patterns may contribute to elevated STI diagnosis rates among England's Black Caribbean (BC) population. We examined the differences between BC and White British/Irish (WBI) sexual health clinic (SHC) attendees' reported partnerships and sexual mixing...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2018-053739
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:To develop and evaluate instructional and packaging materials for patient-delivered partner therapy (PDPT). METHODS:64 patients participated from an urban US sexually transmitted infection (STI) clinic. The research comprised three phases: individual interviews to elicit attitudes and beliefs regarding PDPT ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2008.033746
更新日期:2009-04-01 00:00:00
abstract:OBJECTIVE:To estimate the cumulative incidence of severe complications associated with genital chlamydia infection in the general female population. METHODS:The Uppsala Women's Cohort Study was a retrospective population based cohort study in Sweden, linking laboratory, hospital, and population registers. We estimated...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2005.017186
更新日期:2006-06-01 00:00:00