Abstract:
:We previously reported that RO(+) expression correlated with increased mutation, activation, and selection among human germinal center (GC) B cells. Here, we subdivided human tonsillar B cells, including IgD(-)CD38(+) GC B cells, into different fractions based on RB expression. Although each subset contained RB(+) cells, when used as an intrasubset marker, differential RB expression effectively discriminated between phenotypically distinct cells. For example, RB(+) GC B cells were enriched for activated cells with lower AID expression. RB inversely correlated with mutation frequency, demonstrating a key difference between RB- and RO-expressing GC B cells. Reduced RB expression during the transition from pre-GC (IgM(+)IgD(+)CD38(+)CD27(-)) to GCB cells was followed by a dramatic increase during the GC-to-plasmablast (IgD(-)CD38(++)CD27(+)) and memory (IgD(-)CD38(-)CD27(+)) transition. Interestingly, RB(+) GC B cells showed increased signs of terminal differentiation toward CD27(+) post-GC early plasmablast (increased CD38 and RO) or early memory (decreased CD38 and RO) B cells. We propose that as in T cells, differential RB expression directly correlates with development- and function-based transitions in tonsillar B cells. Application of this RB:RO system should advance our understanding of normal B-cell development and facilitate the isolation of more discrete B-cell populations with potentially different propensities in disease pathogenesis.
journal_name
Bloodjournal_title
Bloodauthors
Jackson SM,Harp N,Patel D,Wulf J,Spaeth ED,Dike UK,James JA,Capra JDdoi
10.1182/blood-2008-03-145979subject
Has Abstractpub_date
2009-04-23 00:00:00pages
3999-4007issue
17eissn
0006-4971issn
1528-0020pii
blood-2008-03-145979journal_volume
113pub_type
杂志文章相关文献
BLOOD文献大全abstract::Many current experimental results show the necessity of new conceptual approaches to understand hematopoietic stem cell organization. Recently, we proposed a novel theoretical concept and a corresponding quantitative model based on microenvironment-dependent stem cell plasticity. The objective of our present work is t...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-01-0282
更新日期:2005-01-15 00:00:00
abstract::A prominent feature of most if not all cancers is a striking genetic instability, leading to ongoing accrual of mutational changes, some of which underlie tumor progression, including acquisition of invasiveness, drug resistance, and metastasis. Thus, the molecular basis for the generation of this genetic diversity in...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-05-089193
更新日期:2009-03-05 00:00:00
abstract::The extravasation of leukocytes from the lumen of the vessel to a site of inflammation requires specific binding events. The interaction of leukocytes with endothelium, via specific receptors, may provide intracellular signals that activate extravasating cells. In the present study, we have investigated the production...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::There are many reports in the literature of blood test abnormalities occurring in patients with venous or arterial thrombosis. Most of these have not used acceptable criteria for establishing an association between thrombosis and blood tests and, therefore, their interpretation is questionable. Recently, sensitive and...
journal_title:Blood
pub_type: 杂志文章,评审
doi:
更新日期:1981-01-01 00:00:00
abstract::We investigated the intracellular site of pro-von Willebrand factor (pro-vWF) cleavage and multimerization, as well as the fate of the propolypeptide (von Willebrand antigen II) after cleavage. Analysis of subcellular fractions of endothelial cells metabolically labeled with sulfate showed that both cleavage and coval...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-06-15 00:00:00
abstract::Cutaneous T-cell lymphoma (CTCL) constitutes a malignant proliferative disease involving mostly CD4(+) T cells arising in the skin. Because of the lack of curative treatment options, interferons (IFN) have been introduced into the therapy of CTCL. Although effective even in advanced disease, response rates were about ...
journal_title:Blood
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:
更新日期:1998-11-15 00:00:00
abstract::Marrow cells of myeloid lineage from 115 patients with myelodysplastic syndrome (MDS) were characterized by multidimensional flow cytometry and compared with findings in 104 patients with various disorders and 25 healthy donors. Based on phenotypic and scatter characteristics, a flow cytometric scoring system (FCSS) w...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-09-2768
更新日期:2003-07-01 00:00:00
abstract::Pulmonary arterial hypertension (PAH) is suspected to be a strong mortality determinant of hemolytic disorders. However, direct contribution of acute intravascular hemolysis to fatal PAH has not been investigated. The roles of nitric oxide (NO) insufficiency and platelet activation in hemolysis-associated fatal PAH ha...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-01-267112
更新日期:2010-09-02 00:00:00
abstract::In this issue of Blood, den Exter et al highlight the importance of subsegmental pulmonary emboli. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2013-06-508440
更新日期:2013-08-15 00:00:00
abstract::An imbalance between cellular apoptosis and survival may be critical for the pathogenesis of lymphoma. Therefore, the gene expression pattern in lymph node preparations from patients with mantle cell lymphoma (MCL) was compared to the pattern in nonmalignant hyperplastic lymph nodes (HLs). Oligonucleotide microarray a...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.3.787
更新日期:2001-08-01 00:00:00
abstract::beta and gamma T cell receptor (TCR) gene configuration was studied in 12 patients with large granular lymphocyte T cell leukemia (LGL-leukemia). Both genes were found rearranged in ten cases. In the remaining two patients TCR beta was found in germline configuration. In one of them rearrangement of T cell-rearranging...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-02-01 00:00:00
abstract::Rauscher murine erythroleukemia cells, grown continuously in vitro, undergo erythroid differentiation in response to the hormone erythropoietin. Therefore, they serve as an important model system with which to examine critical biochemical aspects of this developmental process. Intact, uninduced Rauscher cells possess ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
abstract::In this issue of Blood, Pozzi et al demonstrate that removing an anionic cage promotes exposure of R169 thereby generating a protein C (PC) that is far more readily activated. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2012-05-427401
更新日期:2012-07-19 00:00:00
abstract::Hematopoietic stem cell (HSC) homeostasis is controlled by cytokine receptor-mediated Janus kinase 2 (JAK2) signaling. We previously found that JAK2 is promptly ubiquitinated upon cytokine stimulation. Whether a competing JAK2 deubiquitination activity exists is unknown. LNK is an essential adaptor protein that constr...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2018-10-877563
更新日期:2019-04-04 00:00:00
abstract::Several studies demonstrate that hemolysis and free heme in circulation cause endothelial barrier dysfunction and are associated with severe pathological conditions such as acute respiratory distress syndrome, acute chest syndrome, and sepsis. However, the precise molecular mechanisms involved in the pathology of heme...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019003986
更新日期:2020-08-06 00:00:00
abstract::We used both radiolabeled and fluorescein-labeled antiglobulins in assays to detect antibodies against platelets in multiply transfused patients to determine the value of these tests in predicting the outcome of platelet transfusion in such patients. In 15 allosensitized patients, we studied 68 single-donor platelet t...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::Wnt signaling is involved in numerous aspects of vertebrate development and homeostasis, including the formation and function of blood cells. Here, we show that canonical and noncanonical Wnt signaling pathways are present and functional in megakaryocytes (MKs), with several Wnt effectors displaying MK-restricted expr...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-03-416875
更新日期:2013-01-03 00:00:00
abstract::In B cells, expression of CIITA and resulting major histocompatibility complex II (MHCII) is mediated exclusively by promoter III (CIITA-PIII) activation. Recent studies have established that CIITA-PIII also participates in the expression of CIITA in activated human T cells, dendritic cells, and monocytes. In this stu...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-03-0790
更新日期:2004-11-01 00:00:00
abstract::The production and deployment of phagocytes are central functions of the hematopoietic system. In the 1950s, radioisotopic studies demonstrated the high production rate and short lifespan of neutrophils and allowed researchers to follow the monocytes as they moved from the marrow through the blood to become tissue mac...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2007-12-077917
更新日期:2008-08-15 00:00:00
abstract::Cholesterol, in addition to providing rigidity to the fluid membrane, plays a critical role in receptor function, endocytosis, recycling, and signal transduction. In the present study, we examined the effect of membrane cholesterol on functional expression of tissue factor (TF), a cellular receptor for clotting factor...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-03-0990
更新日期:2005-01-01 00:00:00
abstract::We designed a trial in which postremission therapy of young patients with de novo acute myeloid leukemia (AML) was decided combining cytogenetics/genetics and postconsolidation levels of minimal residual disease (MRD). After induction and consolidation, favorable-risk patients (FR) were to receive autologous stem cell...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood.2018886960
更新日期:2019-09-19 00:00:00
abstract::The prognosis of acute myeloid leukemia (AML) is very poor in elderly patients, especially in those classically defined as having unfavorable cytogenetics. The recent monosomal karyotype (MK) entity, defined as 2 or more autosomal monosomies or combination of 1 monosomy with structural abnormalities, has been reported...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-09-307264
更新日期:2011-07-21 00:00:00
abstract::Hemophilia B is a bleeding disorder caused by a deficiency of clotting factor IX (FIX). A colony of FIX deficient Lhasa Apso dogs has been established and the molecular basis of hemophilia B has been determined. The plasma factor IX levels were < 1% of normal canine levels in affected dogs. A complex deletion mutation...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract::Fifty-one children with acute lymphoblastic leukemia (ALL) in second or subsequent remission after a first remission of at least 24 months underwent purged, autologous bone marrow transplantation (ABMT). Bone marrow was harvested in remission and purged in vitro with monoclonal antibodies specific for leukemia-associa...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-03-15 00:00:00
abstract::Hemophilia B Leyden is an X chromosome-linked bleeding disorder characterized by an altered developmental expression of blood coagulation factor IX. This form of hemophilia B has been found to be associated with a variety of single point mutations in the factor IX promoter region. We now describe a novel point mutatio...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::Using flow cytometric and RNase protection assays, this study examined the expression of chemokine receptors in nonactivated natural killer (NK) cells and compared this expression with NK cells activated with interleukin (IL)-2, which either adhered to plastic flasks (AD) or did not adhere (NA). None of the NK cell su...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.2.367
更新日期:2001-01-15 00:00:00
abstract::In ischemic stroke, treatment options are limited. Therapeutic thrombolysis is restricted to the first few hours after stroke, and the utility of current platelet aggregation inhibitors, including GPIIb/IIIa receptor antagonists, and anticoagulants is counterbalanced by the risk of intracerebral bleeding complications...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2008-04-144758
更新日期:2008-11-01 00:00:00
abstract::The extent and rapidity with which T cells are regenerated from graft-derived precursor cells directly influences the incidence of infection and the T-cell-based graft-versus-tumor effect. Measurement of T-cell receptor excision circles (TRECs) in peripheral blood is a means of quantifying recent thymic T-cell product...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-04-1405
更新日期:2005-01-15 00:00:00
abstract::The response of human myeloid leukemia cells to treatment with 1-beta-arabinofuranosylcytosine (ara-C) includes the induction of apoptosis. Ara-C induced apoptosis is associated with proteolytic cleavage of poly(ADP-ribose) polymerase (PARP) and protein kinase C (PKC) delta. However, the signals involved in this respo...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-09-15 00:00:00
abstract::The aim of this study was to investigate whether interleukin (IL)-6 induces the production of IL-1 and tumor necrosis factor (TNF) antagonists. Serial plasma samples were obtained from cancer patients participating in phase I and II trials of recombinant IL-6 administered as a 120-hour continuous intravenous (IV) infu...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:
更新日期:1994-01-01 00:00:00