Abstract：
:The effect of bucillamine (BA) on glutathione (GSH) and GSH-related enzymes was investigated in C57 mouse. Administration of high doses of BA (150-400 mg/kg) produced a dose-dependent depletion (20-44%) of hepatic GSH, which was similar in magnitude to that produced by equimolar doses of other sulphydryl drugs studied previously. GSH depletion after acute BA administration correlated well with the elevation of serum glutamic-pyruvic transaminase (SGPT) (6-9-fold increase above control). The increase in SGPT after chronic administration (7 days), although significantly higher than the controls, was however much less than after acute administration. The hepatic GSH concentrations of mice given 7 days of BA were similar to the controls, again correlating well with SGPT activity. Administration of BA (150-400 mg/kg) caused also a significant dose-dependent increase in the oxidized glutathione (GSSG) in blood by 2-7-fold, as well as a dose-dependent increase in blood glutathione S-transferase (GST) activity (2-13-fold). In an in vitro experiment, hepatic GST activity was activated by various concentrations of BA (1 microM-1mM). There was little or no effect on GSSG reductase and on glutathione peroxidase (GSH-Px) after acute administration of BA. Chronic administration of BA had no effect on hepatic GSSG reductase and GSH-Px, but GSSG reductase activity in blood was increased significantly by 4-fold. It is possible that BA may affect the redox status through auto-oxidation and oxidation with endogenous thiols such as glutathione, affecting GSH concentrations and the GSH/GSSG ratio in tissues and, thus, having both metabolic and toxicological consequences. Whether or not the induction of GST activity in vivo in blood and in vitro in liver enzyme preparations shared the same underlying mechanism(s) requires further investigation.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Yeung JHdoi
10.1016/0006-2952(91)90045-7subject
Has Abstractpub_date
1991-07-25 00:00:00pages
847-52issue
4eissn
0006-2952issn
1873-2968pii
0006-2952(91)90045-7journal_volume
42pub_type
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pub_type： 杂志文章
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更新日期：1984-02-01 00:00:00
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doi：10.1016/0006-2952(84)90365-4
更新日期：1984-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0006-2952(88)90784-8
更新日期：1988-04-01 00:00:00
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更新日期：1989-03-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1997-05-09 00:00:00
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pub_type： 杂志文章
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pub_type： 临床试验,杂志文章,随机对照试验
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journal_title：Biochemical pharmacology
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1987-09-15 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2003-02-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1998-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：2007-01-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：2003-06-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2015.01.010
更新日期：2015-03-15 00:00:00
