Abstract:
:Extracellular nucleotides have multiple biological actions in processes such as proliferation, differentiation, chemotaxis, and cytokine secretion through P2X receptors on the cell surface. To determine the biological activity of adenosine triphosphate (ATP) and the expression of P2 nucleotide receptors in murine bone marrow-derived hematopoietic cells and stem cells/progenitor cells, we investigated the effects of ATP in assays of cell proliferation and cell death in vitro. Our results demonstrated that several subtypes of P2X receptors were expressed on hematopoietic cells and that P2X7, in particular, was partially expressed in hematopoietic stem cells/progenitor cells. In addition, stimulation of hematopoietic cells with high concentrations of ATP caused severe inhibition of cell proliferation despite the presence of cytokine stimulation. We analyzed the apoptotic effects of stimulation with several different dosages of ATP and confirmed the enhanced apoptotic activity in hematopoietic cells and progenitor cells. Antagonists, against P2X receptors and ATP, suramin and oxidized ATP, inhibited the induction of cell death for murine hematopoietic cells. Our data suggest that extracellular nucleotides may provide a novel and powerful tool for regulating the cell fate of hematopoietic stem cells.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Yoon MJ,Lee HJ,Lee YS,Kim JH,Park JK,Chang WK,Shin HC,Kim DKdoi
10.1248/bpb.30.671subject
Has Abstractpub_date
2007-04-01 00:00:00pages
671-6issue
4eissn
0918-6158issn
1347-5215pii
JST.JSTAGE/bpb/30.671journal_volume
30pub_type
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