Abstract:
BACKGROUND:The T2G1s monoclonal antifibrin antibody binds specifically to fibrin but not to fibrinogen. METHODS AND RESULTS:In a canine model of acute arterial thrombosis, we determined the feasibility of imaging thrombi using a 99mTc-labeled Fab' fragment. In 14 dogs, 10 carotid and 13 femoral artery thrombi were produced using 2-hour temporary occlusion, crush injury, and local thrombin injection methods. A sham-operated carotid artery served as control. Antifibrin antibody was injected intravenously at the end of temporary occlusion. Serial planar radionuclide images were obtained immediately and at 1 and 2 hours. Following killing the dogs at 2 hours, we measured antibody uptake ex vivo in 5-mm-long segments of thrombus, the adjacent injured artery, and a control artery. Antibody was cleared from the blood with a mean +/- SD t1/2 of 121 +/- 23 minutes. The thrombi weighed 218 +/- 140 mg. Antibody uptake in the thrombi was patchy, and the thrombi were closely adherent to the injured arterial wall. In the segment with maximal ex vivo antibody uptake, the ratio of control artery to blood counts/g/sec was 0.65 +/- 0.46, the injured artery-to-blood ratio was 2.35 +/- 1.01 (p less than 0.0001 versus control), and the thrombus-to-blood ratio was 4.24 +/- 2.58 (p less than 0.0001 versus control). In three dogs, an isotype-matched ovarian tumor antibody labeled with 111In was injected with T2G1s but was not taken up in the thrombus or the adjacent arterial wall. Visual analysis of the in vivo carotid radionuclide images showed uptake by 2 hours in all 10 carotid thrombi. Quantitative image analysis, measured as the thrombus-to-opposite carotid artery ratio, showed increasing uptake over time with ratios of 1.1 +/- 0.3, 1.6 +/- 2.0, and 2.2 +/- 1.3 on the immediate, 1-hour, and 2-hour images, respectively. All quantitative ratios of 1.3 or greater were visually identified. CONCLUSIONS:99mTc-labeled Fab' fragments of the T2G1s antibody are taken up specifically by acute arterial thrombi after intravenous injection. Uptake is progressive over a 2-hour period, and all thrombi are detected by radionuclide imaging at 2 hours. These results show that it is feasible to noninvasively detect arterial thrombi within 2 hours of formation.
journal_name
Circulationjournal_title
Circulationauthors
Cerqueira MD,Stratton JR,Vracko R,Schaible TF,Ritchie JLdoi
10.1161/01.cir.85.1.298subject
Has Abstractpub_date
1992-01-01 00:00:00pages
298-304issue
1eissn
0009-7322issn
1524-4539journal_volume
85pub_type
杂志文章相关文献
CIRCULATION文献大全abstract::Cardiac events of early diastole were studied in 50 normal subjects and 46 patients with mitral stenosis (MS) by simultaneous recordings of the mitral valve echogram (MVE), phonocardiogram, and apexcardiogram (ACG). Left ventricular isovolumic relaxation time (IRT), measured between A2 and onset of the MVE opening mot...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.57.4.708
更新日期:1978-04-01 00:00:00
abstract::We tested the hypothesis that different subsets of ischemia-induced wall motion disorders are characterized by specific patterns of abnormal regional left ventricular systolic function. Regional contraction was quantitatively assessed from two-dimensional echocardiograms by an automated integrative analysis considerin...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.76.4.768
更新日期:1987-10-01 00:00:00
abstract:BACKGROUND:Whether reperfusion can cause necrosis of previously viable myocytes (lethal reperfusion injury) remains controversial. Numerous studies examined the ability of various agents to prevent or limit reperfusion injury, but the results were contradictory. In a single-canine-heart model of ischemia-reperfusion, w...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.91.12.2989
更新日期:1995-06-15 00:00:00
abstract:BACKGROUND:Retrospective studies in dialysis patients have reported increased survival after coronary artery bypass (CAB) compared with coronary artery stenting and PTCA. The purpose of this study was to compare the long-term outcome of renal transplant recipients after stent, PTCA, or CAB with or without internal mamm...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.CIR.0000129317.12580.68
更新日期:2004-06-15 00:00:00
abstract:BACKGROUND:The role of angiotensin II (Ang II) type 2 (AT2) receptor in atherosclerosis was explored with the use of AT2 receptor/apolipoprotein E (ApoE)-double-knockout (AT2/ApoE-DKO) mice, with a focus on oxidative stress. METHODS AND RESULTS:After treatment with a high-cholesterol diet (1.25% cholesterol) for 10 we...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.104.525550
更新日期:2005-09-13 00:00:00
abstract::Ultrasonic tissue characterization is a new area of investigation in the field of cardiac ultrasound. The amplitude and frequency of the ultrasound signal are normally altered as the signal penetrates through tissue. It is assumed that the amplitude distribution and frequency shift of diseased or edematous tissue are ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.72.1.193
更新日期:1985-07-01 00:00:00
abstract::Balloon dilation angioplasty was attempted in 10 infants and children with severe congenital or operative "venous" obstructions. In five children the obstructions were "vena caval" and followed repair for transposition of the great vessels (four patients) or orthotopic liver transplantation (one patient). In the four ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.70.3.457
更新日期:1984-09-01 00:00:00
abstract::The prognostic value of abnormalities on the electrocardiogram (ECG) present 1 year after initial myocardial infarction (MI) is examined in relation to reinfarction and coronary death throughout 32 years (mean, 10.1 years) of follow-up in the Framingham Heart Study. Resting 12-lead ECGs were available in 251 survivors...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.81.3.780
更新日期:1990-03-01 00:00:00
abstract::Although coronary thrombus overlying a disrupted atherosclerotic plaque has long been considered the hallmark and the primary therapeutic target for acute myocardial infarction (MI), multiple other mechanisms are now known to cause or contribute to MI. It is further recognized that an MI is just one of many types of a...
journal_title:Circulation
pub_type: 杂志文章,评审
doi:10.1161/CIRCULATIONAHA.119.040631
更新日期:2019-11-12 00:00:00
abstract:BACKGROUND:Development of surgical therapy for aortic aneurysms and dissections has led to treatment of the life-threatening cardiovascular complications associated with Marfan syndrome. The present study determines the effect of surgical therapy on the life expectancy of patients with Marfan syndrome and the clinical ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.91.3.728
更新日期:1995-02-01 00:00:00
abstract::To elucidate the mechanism of regional hemostasis in the left ventricular (LV) cavity during myocardial infarction, the blood pathway in LV cavity was examined with contrast echocardiography injected from the left atrium before and after coronary ligation in nine canines. Before coronary ligation, contrast echoes spre...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.78.1.157
更新日期:1988-07-01 00:00:00
abstract:BACKGROUND:Vascular Ehlers-Danlos syndrome (vEDS), also known as EDS type IV, an inherited disorder of connective tissue, results from mutations in the gene encoding type III procollagen (COL3A1). Affected patients are at risk for arterial dissection or rupture, the main cause of death. To understand the pathogenesis o...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.CIR.0000121741.50315.C2
更新日期:2004-03-30 00:00:00
abstract::Sequential control angiographic examinations were performed at 2 weeks, 1 year, 5 to 7 years, and 10 to 12 years after aortocoronary saphenous vein bypass surgery in 82 unselected patients. Graft modifications consisting of wall irregularities and obstructive lesions of various severity and shapes that were found to d...
journal_title:Circulation
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
abstract:BACKGROUND:Isomerism is associated with a complex spectrum of anomalies. There is paucity of data on prenatally detected cases. METHODS AND RESULTS:Between January 1990 and February 2004, 83 of 166 cases (50%) had a prenatal diagnosis of left isomerism (LAI; 52 of 97) or right isomerism (RAI; 31 of 69) at our institut...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.105.552364
更新日期:2005-10-18 00:00:00
abstract:BACKGROUND:A variable degree of smooth muscle cell (SMC) proliferation after balloon injury has been reported in previous rat studies. It is unknown whether balloon injury induces c-fos expression and whether it is related to the degree of vascular injury in vivo. Therefore, we tested the hypothesis that proportional i...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.92.5.1230
更新日期:1995-09-01 00:00:00
abstract:BACKGROUND:Cardiovascular disease is the leading cause of death for both men and women in the United States and the world. A profound pattern exists in the time of day at which the death occurs; it is in the morning, when the endothelium is most vulnerable and blood pressure surges, that stroke and heart attack most fr...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.108.827477
更新日期:2009-03-24 00:00:00
abstract:BACKGROUND:This study reports on the trends in the volume and outcomes of coronary revascularization procedures performed on Medicare beneficiaries between 2008 and 2012. METHODS AND RESULTS:This retrospective study identifies all Medicare beneficiaries undergoing a coronary revascularization procedure: coronary arter...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.114.012485
更新日期:2015-01-27 00:00:00
abstract:BACKGROUND:Since the clinical introduction of dynamic cardiomyoplasty, a discrepancy has been observed between unchanged measurements of cardiac function and improved clinical outcome. METHODS AND RESULTS:We performed a beat-to-beat analysis of cardiac performance at rest in nine cardiomyoplasty patients 6 to 24 month...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.91.7.2010
更新日期:1995-04-01 00:00:00
abstract::BACKGROUND. The fenestrated Fontan operation has been applied to high-risk patients with univentricular hearts, resulting in improved survival. The purpose of this study was to determine the hemodynamic factors responsible for these improved results. METHODS AND RESULTS. We performed the fenestrated Fontan operation i...
journal_title:Circulation
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract:BACKGROUND:Some clinical data suggest that atrial-based pacing prevents paroxysmal atrial fibrillation (AF). This study tested the hypothesis that DDDR pacing compared with VDD pacing prevents AF after atrioventricular (AV) junction ablation. METHODS AND RESULTS:Patients were randomized to DDDR pacing (n=33) or to VDD...
journal_title:Circulation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1161/01.cir.102.7.736
更新日期:2000-08-15 00:00:00
abstract:BACKGROUND:The renin-angiotensin system regulates blood pressure through its effects on vascular tone, renal hemodynamics, and renal sodium and fluid balance. METHODS AND RESULTS:Using data from a large population-based sample of 1488 siblings having a mean age of 14.8 years and belonging to the youngest generation of...
journal_title:Circulation
pub_type: 评论,杂志文章
doi:10.1161/01.cir.97.18.1773
更新日期:1998-05-12 00:00:00
abstract:BACKGROUND:Activation of protein kinase C-linked receptors and subsequent opening of the mitochondrial ATP-sensitive K(+) (mitoK(ATP)) channel are crucial in preconditioning (PC). This study examined whether postinfarct ventricular remodeling interferes with the PC mechanism. METHODS AND RESULTS:Two weeks before isola...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.102.4.458
更新日期:2000-07-25 00:00:00
abstract:BACKGROUND:Proposed advantages to the Ross procedure included presumed increased freedom from reoperation and simpler reoperation for pulmonary conduit replacement if needed. It is increasingly apparent, however, that reoperations are frequent after the Ross procedure and that when required, they may be more complex th...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.109.897538
更新日期:2010-09-21 00:00:00
abstract:BACKGROUND:During septic shock, the vasoconstrictor response to norepinephrine is seriously blunted. Animal experiments suggest that hyperpolarization of smooth muscle cells by opening of potassium (K) channels underlies this phenomenon. In the present study, we examined whether K-channel blockers and/or nitric oxide (...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/CIRCULATIONAHA.105.590232
更新日期:2006-08-01 00:00:00
abstract::It has been suggested that the beneficial effects of reperfusing ischemic myocardium might be in part reversed by the occurrence of "reperfusion injury." One possible mechanism could be the generation of oxygen free radicals. Superoxide dismutase enzymatically scavenges superoxide radicals by dismutation to hydrogen p...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.75.1.282
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND:Insulin sensitivity varies up to threefold in apparently healthy individuals, but the mechanism for this is unknown. We have examined the hypothesis that vascular endothelial nitric oxide production and insulin sensitivity are directly related in humans. METHODS AND RESULTS:Nineteen healthy male subjects we...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.93.7.1331
更新日期:1996-04-01 00:00:00
abstract:BACKGROUND:Platelet activation plays a pivotal role in the pathogenesis of acute coronary disease. Monocytes are involved in the progression of atherosclerosis and are potent activators of blood coagulation through their ability to synthesize tissue factor (TF). The aim of this study was to compare markers of monocyte ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.90.4.1662
更新日期:1994-10-01 00:00:00
abstract:BACKGROUND:Surviving cells in the postinfarction border zone are subjected to intense fluctuations of their microenvironment. Recently, border zone cardiomyocytes have been specifically implicated in cardiac regeneration. Here, we defined their unique transcriptional and regulatory properties, and comprehensively valid...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.118.038944
更新日期:2019-09-09 00:00:00
abstract::Both the Veterans Administration Cooperative Study and the European Coronary Surgery Study have provided only brief accounts of graft patency rates in their surgically treated patients. In the Veterans Administration Cooperative Study, at an average of 1 year after operation, 69% of the grafts were patent among 208 pa...
journal_title:Circulation
pub_type: 临床试验,杂志文章,评审
doi:
更新日期:1985-12-01 00:00:00
abstract:BACKGROUND:Vascular events during initial reperfusion after a period of hypothermic cardioplegic ischemia may play an important role in the recovery of mechanical function. Prior studies have shown (1) that dysfunction of the postischemic myocardium after normothermic ischemia can be ameliorated by vasodilation and (2)...
journal_title:Circulation
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00