Abstract:
:Thymidine phosphorylase (TP), also known as platelet-derived endothelial cell growth factor (PD-ECGF), has been implicated in bladder cancer angiogenesis and invasion. However, the molecular basis of its role in invasion remains unclear. We investigated the expression of TP and 10 invasion-related genes in bladder cancers from 72 randomly selected patients by real-time two-step RT-PCR assay. We found that the expression levels of TP, MMP-9, uPA, and MMP-2 were significantly higher in invasive tumors than those in superficial tumors. Also, the expression level of TP significantly correlated with that of uPA, MMP-1, MMP-9, PAI-1 and VEGF. KK47/TP cells, bladder cancer cells that overexpress TP, had a higher expression of MMP-7 and MMP-9 than KK/CV cells that express lower level of TP in hypoxic condition. PC/TP cells, prostate cancer cells that overexpress TP, also had a higher expression of MMP-1 and MMP-7 than PC/CV cells that express no detectable TP. Taken together these data indicate that TP enhances the invasion of tumor cells through the induction of invasion-related genes.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Gotanda T,Haraguchi M,Tachiwada T,Shinkura R,Koriyama C,Akiba S,Kawahara M,Nishiyama K,Sumizawa T,Furukawa T,Mimata H,Nomura Y,Akiyama S,Nakagawa Msubject
Has Abstractpub_date
2006-06-01 00:00:00pages
1085-91issue
6eissn
1107-3756issn
1791-244Xjournal_volume
17pub_type
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