Limitations of the fast green assay for chemosensitivity testing in human lung cancer.

Abstract:

:Selection of patients with lung cancer who are most likely to benefit from chemotherapeutic treatment would be a substantial step forward. Therefore, a prospective study in predictive chemosensitivity testing in vitro using the fast green assay (FGA) as developed by Weisenthal et al was carried out. Sixty-six pretreatment tumor specimens were obtained, the majority by means of bronchoscopy (n = 42). Due to an initially insufficient yield of tumor cells (n = 19), dead cells in control samples after four-day culture (n = 15), contamination (n = 7), and laboratory failure (n = 2), only 23 (34.8 percent) samples were successfully tested. In 14 of 36 patients, a comparison between in vitro and in vivo response was possible. Taking into account the number of failures, this number of successful assays does not allow for any conclusion regarding accuracy of the FGA. We conclude that the FGA has limited usefulness for in vitro chemosensitivity testing in patients with disseminated lung cancer in whom biopsy specimens were taken without major surgical investigations. Future directions for predictive testing in vitro are discussed.

journal_name

Chest

journal_title

Chest

authors

Smit EF,de Vries EG,Meijer C,Mulder NH,Postmus PE

doi

10.1378/chest.100.5.1358

subject

Has Abstract

pub_date

1991-11-01 00:00:00

pages

1358-63

issue

5

eissn

0012-3692

issn

1931-3543

pii

S0012-3692(16)33691-1

journal_volume

100

pub_type

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