Abstract：
:A novel human mammary epithelial cell line, HME348, was established from benign breast tissue from a 44-year-old germ-line BRCA2 mutation carrier with a history of stage 1 breast cancer. Mutation analysis showed that the patient had a known 6872del4 BRCA2 heterozygous mutation. The human mammary epithelial cells passaged in culture exhibited cellular replicative aging as evidenced by telomere shortening, lack of telomerase activity, and senescence. Ectopic expression of telomerase (hTERT) reconstituted telomerase activity in these cells and led to the immortalization of the cells. When grown on glass, the majority of immortalized HME348 cells expressed ESA and p63 with a small population also expressing EMA. In three-dimensional Matrigel culture, HME348 cells formed complex branching acini structures that expressed luminal (EMA, CK18) and myoepithelial (p63, CALLA, CK14) markers. Three clones derived from this culture were also p63(+)/ESA(+)/EMA(+/-) on glass but formed similar acinar structures with both luminal and myoepithelial cell differentiation in Matrigel confirming the mammary progenitor nature of these cells. Additionally, the experimentally immortalized HME348 cells formed acini in cleared mammary fat pads in vivo. As this is the first report establishing and characterizing a benign human mammary epithelial cell line derived from a BRCA2 patient without the use of viral oncogenes, these cells may be useful for the study of BRCA2 function in breast morphogenesis and carcinogenesis.
journal_name
Breast Cancer Res Treatjournal_title
Breast cancer research and treatmentauthors
Lewis CM,Herbert BS,Bu D,Halloway S,Beck A,Shadeo A,Zhang C,Ashfaq R,Shay JW,Euhus DMdoi
10.1007/s10549-006-9189-9subject
Has Abstractpub_date
2006-09-01 00:00:00pages
103-15issue
1eissn
0167-6806issn
1573-7217journal_volume
99pub_type
杂志文章abstract：:The effects of pituitary adenylate cyclase activating polypeptide (PACAP) analogs were investigated using breast cancer cells. 125I-PACAP-27 bound with high affinity (Kd = 5 nM) to T47D cells (Bmax = 29,000 per cell). Specific 125I-PACAP-27 binding was inhibited half maximally by PACAP-27, PACAP-38, PACAP(6-38) and PA...
journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1023/a:1006262611290
更新日期：1999-07-01 00:00:00
abstract：:Ductal carcinoma in situ (intraductal carcinoma) of the breast is a commonly recognized and curable clinical entity. Patients with intraductal carcinoma are at risk to develop invasive breast cancer presumably due to a transition from the noninvasive to the invasive phase of growth. Primary breast malignancies commonl...
journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/BF00689717
更新日期：1995-06-01 00:00:00
abstract：:We have examined a family with a germline BRCA2 mutation in three cases of invasive breast cancer and one case of ductal carcinoma in situ (DCIS). Loss of heterozygosity (LOH) has been demonstrated at the BRCA2 locus in all cases. This result may suggest that the germline mutation in BRCA2 is the initiating step of DC...
journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-004-9503-3
更新日期：2004-10-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-016-3984-8
更新日期：2016-11-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章,meta分析
doi：10.1007/s10549-012-2343-7
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:The estrogen receptor alpha (ESR1) is a mediator of estrogen response in the breast. The most studied variants in this gene are the PvuII and XbaI polymorphisms, which have been associated to lower sensitivity to estrogen. We evaluated whether these polymorphisms were associated with breast cancer risk by me...
journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-007-9562-3
更新日期：2008-02-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-012-2377-x
更新日期：2013-01-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-015-3364-9
更新日期：2015-04-01 00:00:00
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journal_title：Breast cancer research and treatment
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更新日期：2018-08-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-019-05485-5
更新日期：2020-02-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-011-1534-y
更新日期：2012-02-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1023/a:1006035603635
更新日期：1998-09-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章,多中心研究
doi：10.1007/s10549-010-1200-9
更新日期：2011-01-01 00:00:00
abstract：:Toremifene is a triphenylethylene derivative structurally and pharmacologically similar to tamoxifen. This Phase I trial assessed the safety, pharmacokinetics, anti-estrogenic, and estrogenic effects of toremifene at six dose levels (10, 20, 40, 60, 200, and 400 mg/day). The most common side-effects associated with th...
journal_title：Breast cancer research and treatment
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1007/BF01807140
更新日期：1990-08-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-008-0125-z
更新日期：2009-07-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-018-4985-6
更新日期：2019-01-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-018-4720-3
更新日期：2018-07-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章,meta分析,评审
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更新日期：2009-09-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-006-9173-4
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/BF00666043
更新日期：1995-01-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-009-0354-9
更新日期：2009-12-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章,评审
doi：10.1007/s10549-008-0268-y
更新日期：2009-07-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/BF01806794
更新日期：1986-01-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 临床试验,杂志文章
doi：10.1007/s10549-009-0541-8
更新日期：2010-04-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-010-0828-9
更新日期：2011-01-01 00:00:00
abstract：PURPOSE:Triple-negative breast cancers (TNBC) are aggressive tumours that exhibit abundant lymphoid infiltrates which modulate tumour behaviour. Recent findings suggest that TNBC with higher densities of plasma cells are associated with a favourable prognosis, and tertiary lymphoid structures (TLS) have prognostic sign...
journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-020-05548-y
更新日期：2020-04-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1023/a:1019969730552
更新日期：2002-10-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1023/a:1006326513176
更新日期：1999-12-01 00:00:00
abstract：:The epidermal growth factor (EGF) is one of several growth factors involved in normal breast epithelial development and tumor proliferation. EGF and EGF-like peptide TGF alpha bind and activate the same membrane receptor protein. This receptor (EGF-R) has been recently studied in breast tumor biopsies and its detectab...
journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/BF01834623
更新日期：1992-03-01 00:00:00
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journal_title：Breast cancer research and treatment
pub_type： 杂志文章
doi：10.1007/s10549-006-9492-5
更新日期：2007-12-01 00:00:00
