Abstract:
:We synthesized modified 2'-deoxyuridine triphosphates bearing amino acids at the C5 position and investigated their substrate properties for KOD Dash DNA polymerase during polymerase chain reaction (PCR). PCR using C5-modified dUTP having an amino acyl group (arginyl, histidyl, lysyl, phenylalanyl, tryptophanyl, leucyl, prolyl, glutaminyl, seryl, O-benzyl seryl or threonyl group) gave the corresponding full-length PCR products in good yield. Although dUTP analogues bearing aspartyl, glutamyl or cysteinyl were found to be poor substrates for PCR catalyzed by KOD Dash DNA polymerase, optimization of the reaction conditions resulted in substantial generation of full-length product. In the case of reaction using dUTP analogue having a cysteinyl group, addition of a reducing agent improved the reaction yield. Thus, PCRs using KOD Dash DNA polymerase together with amino acyl dUTP provide convenient and efficient preparation of various modified DNA libraries with potential protein-like activities.
journal_name
Bioorg Med Chemjournal_title
Bioorganic & medicinal chemistryauthors
Kuwahara M,Hanawa K,Ohsawa K,Kitagata R,Ozaki H,Sawai Hdoi
10.1016/j.bmc.2005.11.030subject
Has Abstractpub_date
2006-04-15 00:00:00pages
2518-26issue
8eissn
0968-0896issn
1464-3391pii
S0968-0896(05)01101-6journal_volume
14pub_type
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