A simple precipitation method for measuring sialic acid in apolipoprotein B-containing lipoproteins in plasma.

abstract：:Background Proglucagon-derived hormones represent a family of peptides mainly produced in the pancreas and the intestine. While several proglucagon-derived peptides play key roles in metabolic diseases, little is known about glicentin. The aim of the present study was to investigate serum glicentin concentrations in i...

journal_title：Annals of clinical biochemistry

authors： Raffort J,Panaïa-Ferrari P,Lareyre F,Blois M,Bayer P,Staccini P,Fénichel P,Chinetti G

更新日期：2018-03-01 00:00:00

abstract：:A high-pressure liquid chromatography procedure for the measurement of free catecholamines in urine is described. 'On-line' sample preparation using a cartesian robot combined with the ASTED process ensures minimal operator intervention and reduced costs. ...

journal_title：Annals of clinical biochemistry

authors： Green B,Cooper JD,Turnell DC

更新日期：1989-07-01 00:00:00

abstract：BACKGROUND:Placental growth factor (PlGF) and soluble-fms-like tyrosine kinase 1 (sFlt-1) are biomarkers of placental function used to aid the diagnosis and prediction of pregnancy complications. This work verified the analytical performance of both biomarkers and provides preliminary diagnostic accuracy data to identi...

journal_title：Annals of clinical biochemistry

authors： Armstrong-Buisseret LK,Haslam S,James T,Bradshaw L,Heazell AE

更新日期：2020-05-01 00:00:00

abstract：:The number of known inherited metabolic disorders resulting in an organic aciduria has increased steadily over the past two decades. Prompt and reliable detection is both clinically and technically demanding but is essential if appropriate treatment is to be undertaken. This is the first study of laboratory performanc...

journal_title：Annals of clinical biochemistry

authors： Bonham JR,Downing M,Pollitt RJ,Manning NJ,Carpenter KH,Olpin SE,Allen JC,Worthy E

更新日期：1994-03-01 00:00:00

abstract：:Background We investigated the in vitro effects of various phospholipids as emulsifiers on the hydrolysing activities of lipoprotein lipase (LPL) Arg243His against triolein as substrate. LPL Arg243His, identified in a patient with hyperchylomicronaemia, displays severely diminished activity for triolein when emulsifie...

journal_title：Annals of clinical biochemistry

authors： Yamaguchi T,Murano T,Tatsuno I,Hiruta N,Suzuki T,Sawada S,Katagiri H,Shirai K,Schneider WJ,Bujo H

更新日期：2017-11-01 00:00:00

abstract：BACKGROUND:Hypercholesterolemia (plasma cholesterol concentration â¥5.2 mmol/L) is a risk factor for cardiovascular disease and stroke. Many different cholesterol self-tests are readily available at general stores, pharmacies and web shops. However, there is limited information on their analytical and diagnostic perfor...

journal_title：Annals of clinical biochemistry

authors： Kurstjens S,Gemen E,Walk S,Njo T,Krabbe J,Gijzen K,Elisen MGLM,Kusters R

更新日期：2021-01-21 00:00:00

abstract：:The mechanisms of hypercalcaemia were assessed in 20 hypercalcaemic patients with breast cancer. Abnormalities suggestive of a PTH-related peptide (PTHrP) mechanism were observed in up to 60% of cases; urinary cyclic adenosine monophosphate (UcAMP) was elevated in nine patients (45%), renal tubular reabsorption of cal...

journal_title：Annals of clinical biochemistry

authors： Gallacher SJ,Fraser WD,Patel U,Logue FC,Soukop M,Boyle IT,Ralston SH

更新日期：1990-11-01 00:00:00

abstract：:Sequential measurements of plasma alkaline phosphatase activity were made in 84 pre-term infants delivered before 38 weeks gestation. In 67% of infants, without evidence of rickets but in whom three or more measurements were made and the peak activity was less than 10 times the adult reference range, the activity rose...

journal_title：Annals of clinical biochemistry

authors： Walters EG,Murphy JF,Henry P,Gray OP,Elder GH

更新日期：1986-11-01 00:00:00

abstract：:The diagnostic performance of a new enzyme linked immunosorbent assay for the cardiac structural protein troponin T in the differential diagnosis of ischaemic cardiac damage was assessed. A well documented set of patients admitted to the coronary care unit of a district general hospital were studied. At a cutoff value...

journal_title：Annals of clinical biochemistry

authors： Collinson PO,Moseley D,Stubbs PJ,Carter GD

更新日期：1993-01-01 00:00:00

abstract：:A bloodspot assay for testosterone has been developed using an antiserum raised against testosterone-3-carboxymethyloxime-bovine serum albumin (T-3 CMO-BSA) conjugate and 125I-T-3CMO-histamine tracer. The method has a detection limit of 0.4 nmol/L with between-batch precision of less than 15% for testosterone values b...

journal_title：Annals of clinical biochemistry

authors： Walker J,Hughes IA,Wood PJ

更新日期：1999-07-01 00:00:00

abstract：:Parenteral nutrition is a well-established technique for feeding patients with intestinal failure and other conditions which render enteral feeding impossible. Biochemical tests are required in the initial assessment of these patients, for monitoring their response to treatment and for the detection and management of ...

journal_title：Annals of clinical biochemistry

authors： Marshall WJ,Mitchell PE

更新日期：1987-07-01 00:00:00

abstract：BACKGROUND:We evaluated procalcitonin (PCT) assay in the emergency diagnosis of neonatal bacterial infection, especially in preterm infants, relative to C-reactive protein (CRP) and fibrinogen. METHODS:One hundred and twenty neonates (32 preterm), of whom 21 were infected, were tested. RESULTS:Concentrations of PCT, ...

journal_title：Annals of clinical biochemistry

authors： Guibourdenche J,Bedu A,Petzold L,Marchand M,Mariani-Kurdjian P,Hurtaud-Roux MF,Aujard Y,Porquet D

更新日期：2002-03-01 00:00:00

abstract：BACKGROUND:Haemoglobin A1c (HbA1c) is the only measure of glycaemic control used for many patients with diabetes, but it has limitations and might sometimes be misleading. HbA(1c) concentrations are influenced by conditions that alter red-cell life and there is evidence that biochemical variation in intracellular glyca...

journal_title：Annals of clinical biochemistry

authors： Macdonald DR,Hanson AM,Holland MR,Singh BM

更新日期：2008-07-01 00:00:00

abstract：:Measurement of urine-free (unconjugated) cortisol (UFC) excretion is widely used in the investigation of hypercortisolaemia. We have measured 24 h UFC excretion in normal healthy individuals using a radioimmunoassay (RIA) method claimed to be suitable for unextracted urine. Significantly higher rates of excretion were...

journal_title：Annals of clinical biochemistry

authors： Lamb EJ,Noonan KA,Burrin JM

更新日期：1994-09-01 00:00:00

abstract：:A rapid vacuum-driven procedure, using pre-treated Sep-Pak C18 cartridges, has been developed for the simultaneous extraction of arginine vasopressin (AVP) and atrial natriuretic peptide (ANP) from plasma. Non-specific interference was removed by fractional elution with an aqueous methanol/trifluoroacetic acid (TFA) m...

journal_title：Annals of clinical biochemistry

authors： Penney MD,Hampton D,Oleesky DA,Livingstone C,Mulkerrin E

更新日期：1992-11-01 00:00:00

abstract：:An automated assay for serum amylase is described. Using the AutoAnalyzer II the method employs, as substrate, a solution of dyed amylopectin which is commercially available (DyAmyl-L). After incubation of serum and substrate at 55 degrees C, 1.0 mol/lKOH is introduced and the coloured products of enzymic hydrolysis a...

journal_title：Annals of clinical biochemistry

authors： Carney JA,Osibeluwo SA,Clements JA,Jay RF

更新日期：1977-11-01 00:00:00

abstract：BACKGROUND AND AIM:Familial hypercholesterolaemia is caused by variants in the low-density lipoprotein cholesterol metabolic pathway involving LDLR, APOB and PCSK9 genes. A national genetic testing service in Wales, UK has observed that no familial hypercholesterolaemia variant is found in almost 80% patients with the ...

journal_title：Annals of clinical biochemistry

authors： Ashfield-Watt P,Haralambos K,Edwards R,Townsend D,Gingell R,Wa Li K,Humphries SE,McDowell I

更新日期：2019-01-01 00:00:00

abstract：:A rapid gas liquid chromatography method has been developed which is suitable for emergency estimations of paracetamol in overdose. The method is compared with a standard ultraviolet method. ...

journal_title：Annals of clinical biochemistry

authors： Stewart MJ,Willis RG

更新日期：1975-01-01 00:00:00

abstract：:Serum osteocalcin did not show any response to the onset of osteosarcoma in Paget's disease of bone whereas serum alkaline phosphatase increased rapidly. This suggests that osteocalcin is not useful in the diagnosis and management of Paget's osteosarcoma and does not reflect the same osteoblastic processes in bone as ...

journal_title：Annals of clinical biochemistry

authors： Davie MW,Worsfold M,Sharp CA

更新日期：1991-03-01 00:00:00

abstract：:In a group of 121 adult patients with end-stage chronic renal failure who had been undergoing maintenance haemodialysis for up to 10 years a number of biochemical variables have been measured and related to a set of objective radiological changes in the same patients. The changes in plasma calcium, magnesium, phosphat...

journal_title：Annals of clinical biochemistry

authors： Moorhead JF,Varghese Z,Tatler GL,Baillod RA,Wills MR

更新日期：1975-05-01 00:00:00

abstract：BACKGROUND:As lifespan in HIV infection increases, cardiovascular disease has emerged as a cause of morbidity and mortality. Asymmetric dimethylarginine is an established marker of endothelial dysfunction and predicts cardiovascular events. The role of asymmetric dimethylarginine in HIV-related cardiovascular disease h...

journal_title：Annals of clinical biochemistry

authors： Hudson CL,Zemlin AE,Ipp H

更新日期：2014-09-01 00:00:00

abstract：:Direct assays have been described for the estimation of progesterone in serum and saliva. Both methods showed good inter-assay precision over a year, and the serum assay is now being used routinely in clinical laboratories. A highly significant correlation was obtained for progesterone concentrations in matched serum ...

journal_title：Annals of clinical biochemistry

authors： Webley GE,Edwards R

更新日期：1985-11-01 00:00:00

abstract：:Addison's disease is a relatively common disorder to endocrinologists, but is rare and potentially fatal when presenting acutely. Treatment now involves replacement of glucocorticoids and mineralocorticoids with synthetic compounds, although historically patients took common salt and plant-based preparations. We descr...

journal_title：Annals of clinical biochemistry

authors： Cooper H,Bhattacharya B,Verma V,McCulloch AJ,Smellie WS,Heald AH

更新日期：2007-07-01 00:00:00

abstract：:Heterophilic antibodies are well described, but poorly appreciated interferents and is often not a recognized problem affecting most immunoassays. We report a patient presented with ectopic Cushing's syndrome (CS), but repeated plasma adrenocorticotropic hormone (ACTH) concentrations conducted by immunoassay were inap...

journal_title：Annals of clinical biochemistry

authors： Saiegh L,Odeh M,Chen-Konak L,Elias N,Sheikh-Ahmad M,Reut M,Slobodin G,Bejar J,Shechner C

更新日期：2014-07-01 00:00:00

abstract：:The Bhattacharya method and the 'average of normals' method for internal quality control were compared. Both are based on unselected patient test results. The Bhattacharya method is mostly used in clinical chemistry for calculation of reference intervals but could be modified for quality control procedures. Using the ...

journal_title：Annals of clinical biochemistry

authors： Oosterhuis WP,Modderman TA,Dinkelaar RB,Zwinderman AH,van der Helm HJ

更新日期：1991-07-01 00:00:00

abstract：:We evaluated a new immunoselective enzyme assay of bone-specific alkaline phosphatase (ALP). The monoclonal antibody used in this assay was raised against purified bone-specific ALP obtained from SAOS-2 human osteosarcoma cell line. Calibration was based on the enzyme's own activity. The relative activity of the antib...

journal_title：Annals of clinical biochemistry

authors： Hata K,Tokuhiro H,Nakatsuka K,Miki T,Nishizawa Y,Morii H,Miura M

更新日期：1996-03-01 00:00:00

abstract：:Study of the glycoprotein hormones, including hCG, is complex and evolving, and has benefited from recent major advances in analytical technology and molecular biology. It is important to be aware of the effect that these technological advances have, both on the analytical and the clinical requirements for provision o...

journal_title：Annals of clinical biochemistry

authors： Sturgeon CM,McAllister EJ

更新日期：1998-07-01 00:00:00

abstract：:The last 10 years have witnessed radical changes in the definition and diagnosis of the acute coronary syndrome (ACS), including myocardial infarction, as a result of the introduction of sensitive and specific markers of myocardial damage, the cardiac troponins. One barrier to the universal acceptance of these markers...

journal_title：Annals of clinical biochemistry

authors： Lamb EJ,Webb MC,Abbas NA

更新日期：2004-01-01 00:00:00

abstract：:The diagnostic usefulness of fasting total serum bile acids (SBA/F) in the detection of liver diseases and assessment of different aspects of hepatic function alteration was evaluated in 61 healthy subjects and 186 patients with liver disease. The value of SBA/F was compared with other routine tests. In 49 healthy sub...

journal_title：Annals of clinical biochemistry

authors： Cravetto C,Molino G,Biondi AM,Cavanna A,Avagnina P,Frediani S

更新日期：1985-11-01 00:00:00

abstract：:We assayed interleukin 6 (IL-6) concentrations in cerebrospinal fluid (CSF) from patients affected by meningitis of different aetiologies, and verified whether IL-6 can be used as a diagnostic marker in the differential diagnosis of meningitis. We used a monoclonal antibody enzyme immunoassay to test 98 CSF samples cl...

journal_title：Annals of clinical biochemistry

authors： López-Cortés LF,Cruz-Ruiz M,Gómez-Mateos J,Jiménez-Hernández D,Viciana-Fernández P,Jiménez-Mejías E

更新日期：1997-03-01 00:00:00