Insulinlike growth factor-I-mediated migration and invasion of human colon carcinoma cells requires activation of c-Met and urokinase plasminogen activator receptor.

Abstract:

OBJECTIVE:To determine whether insulinlike growth factor-I (IGF-I) and hepatocyte growth factor (HGF) cooperate to induce migration and invasion of human colorectal carcinoma (CRC) cells and whether the effects of IGF-I and/or HGF are mediated through activation of the urokinase plasminogen activator (uPA)/uPA receptor (uPAR) system, a central mediator of tumor-cell migration and invasion. SUMMARY BACKGROUND DATA:CRC cells must invade through the basement membrane of the colon and migrate to form metastases. CRC cells are known to overexpress IGF-I receptor (IGF-IR), c-Met, and uPAR, 3 cell-surface receptors known to mediate cell migration and invasion. We hypothesized that IGF-IR and c-Met cooperate to induce migration and invasion in CRC cells and that this signaling is dependent on uPAR. METHODS:KM12L4 human CRC cells were treated with IGF-I, HGF, or IGF-I + HGF in transwell migration and invasion chambers; cells that had migrated or invaded were counted. To determine the role of c-Met in IGF-I-induced migration and invasion, c-Met was inhibited by infection of cells with an adenovirus containing a c-Met ribozyme; transwell assays were then repeated. To determine the role of the uPA/uPAR system in IGF-I-induced CRC cell migration and invasion, transwell assays were repeated after pretreating cells with the uPA inhibitor amiloride or with neutralizing antibodies to uPA and uPAR. RESULTS:IGF-I and HGF, alone or in combination, increased cell migration and invasion. The c-Met ribozyme inhibited IGF-I- and HGF-mediated migration and invasion, indicating that c-Met is essential for these processes. uPA and uPAR inhibition blocked IGF-I- and HGF-mediated migration and invasion, suggesting that uPAR is downstream of IGF/IGF-IR and HGF/c-Met in the signaling pathways that mediate cell migration and invasion. CONCLUSIONS:IGF-I and HGF cooperate to induce migration and invasion of CRC cells, and c-Met and uPA/uPAR are required for IGF-I-mediated migration and invasion. In our in vitro model of CRC migration and invasion, uPA and uPAR appear to be downstream of IGF-IR and c-Met and are required for migration and invasion. Elucidation of the pathways that contribute to tumor progression and metastasis should provide a foundation for the rational development and use of targeted therapies for CRC.

journal_name

Ann Surg

journal_title

Annals of surgery

authors

Bauer TW,Fan F,Liu W,Johnson M,Parikh NU,Parry GC,Callahan J,Mazar AP,Gallick GE,Ellis LM

doi

10.1097/01.sla.0000160699.59061.92

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

748-56; discussion 756-8

issue

5

eissn

0003-4932

issn

1528-1140

pii

00000658-200505000-00008

journal_volume

241

pub_type

杂志文章
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    abstract::The effect of reperfusion following hemorrhagic shock on splanchnic prostanoid release was studied. Anesthetized male rats were bled to a mean arterial blood pressure of 30 mmHg for 30 minutes and either killed or treated with shed blood for 60 minutes and then killed. The superior mesenteric arterial bed was cannulat...

    journal_title:Annals of surgery

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    doi:10.1097/00000658-199012000-00006

    authors: Myers SI,Taylor BJ,Stanislawska M

    更新日期:1990-12-01 00:00:00

  • Clinical and therapeutic features of nonpostoperative nosocomial intra-abdominal infections.

    abstract:OBJECTIVE:To compare the clinical, microbiological, and therapeutic features of nonpostoperative nosocomial intra-abdominal infections (non-PostopNAI) with community-acquired intra-abdominal infections (CAI). SUMMARY BACKGROUND DATA:Prospective (June 2000 through January 2001) consecutive case series analysis of patie...

    journal_title:Annals of surgery

    pub_type: 杂志文章,多中心研究

    doi:10.1097/01.sla.0000114214.68169.e9

    authors: Montravers P,Chalfine A,Gauzit R,Lepape A,Pierre Marmuse J,Vouillot C,Martin C

    更新日期:2004-03-01 00:00:00

  • A Clinical Feasibility Trial for Identification of Exceptional Responders in Whom Breast Cancer Surgery Can Be Eliminated Following Neoadjuvant Systemic Therapy.

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    journal_title:Annals of surgery

    pub_type: 临床试验,杂志文章

    doi:10.1097/SLA.0000000000002313

    authors: Kuerer HM,Rauch GM,Krishnamurthy S,Adrada BE,Caudle AS,DeSnyder SM,Black DM,Santiago L,Hobbs BP,Lucci A Jr,Gilcrease M,Hwang RF,Candelaria RP,Chavez-MacGregor M,Smith BD,Arribas E,Moseley T,Teshome M,Miggins MV,Vale

    更新日期:2018-05-01 00:00:00

  • The impact of Clostridium difficile on a surgical service: a prospective study of 374 patients.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-199802000-00021

    authors: Kent KC,Rubin MS,Wroblewski L,Hanff PA,Silen W

    更新日期:1998-02-01 00:00:00

  • Unique Tracheal Fluid MicroRNA Signature Predicts Response to FETO in Patients With Congenital Diaphragmatic Hernia.

    abstract:OBJECTIVE AND BACKGROUND:Our objective was to determine the fetal in vivo microRNA signature in hypoplastic lungs of human fetuses with severe isolated congenital diaphragmatic hernia (CDH) and changes in tracheal and amniotic fluid of fetuses undergoing fetoscopic endoluminal tracheal occlusion (FETO) to reverse sever...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0000000000001054

    authors: Pereira-Terra P,Deprest JA,Kholdebarin R,Khoshgoo N,DeKoninck P,Munck AA,Wang J,Zhu F,Rottier RJ,Iwasiow BM,Correia-Pinto J,Tibboel D,Post M,Keijzer R

    更新日期:2015-12-01 00:00:00

  • Trainee Involvement in Ivor Lewis Esophagectomy Does Not Negatively Impact Outcomes.

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    pub_type: 杂志文章

    doi:10.1097/SLA.0000000000002047

    authors: Phillips AW,Dent B,Navidi M,Immanuel A,Griffin SM

    更新日期:2018-01-01 00:00:00

  • A decade's experience with quality improvement in cardiac surgery using the Veterans Affairs and Society of Thoracic Surgeons national databases.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-200110000-00006

    authors: Grover FL,Shroyer AL,Hammermeister K,Edwards FH,Ferguson TB Jr,Dziuban SW Jr,Cleveland JC Jr,Clark RE,McDonald G

    更新日期:2001-10-01 00:00:00

  • Desmoid tumors in familial polyposis coli.

    abstract::Desmoid tumors are locally invasive, nonmetastasizing fibrous tumors most frequently seen in patients with familial polyposis coli (FPC). Of 325 patients with FPC treated at the Cleveland Clinic, 29 (8.9%) were found to have a total of 36 desmoid tumors. These tumors occur in young patients (mean age: 29.8 years), par...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-198607000-00014

    authors: Jones IT,Jagelman DG,Fazio VW,Lavery IC,Weakley FL,McGannon E

    更新日期:1986-07-01 00:00:00

  • Endovascular stent graft repair of abdominal and thoracic aortic aneurysms: a ten-year experience with 817 patients.

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    journal_title:Annals of surgery

    pub_type: 杂志文章,多中心研究

    doi:10.1097/01.sla.0000090473.63393.e9

    authors: Marin ML,Hollier LH,Ellozy SH,Spielvogel D,Mitty H,Griepp R,Lookstein RA,Carroccio A,Morrissey NJ,Teodorescu VJ,Jacobs TS,Minor ME,Sheahan CM,Chae K,Oak J,Cha A

    更新日期:2003-10-01 00:00:00

  • The effects of metoclopramide on postoperative ileus. A randomized double-blind study.

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    journal_title:Annals of surgery

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1097/00000658-197907000-00006

    authors: Davidson ED,Hersh T,Brinner RA,Barnett SM,Boyle LP

    更新日期:1979-07-01 00:00:00

  • Carcinoid tumors of the rectum: a multi-institutional international collaboration.

    abstract:OBJECTIVE:This study aims to describe recent experience with rectal carcinoids in European and North American centers. BACKGROUND:While considered indolent, the propensity of carcinoids to metastasize can be significant. METHODS:Rectal carcinoid patients were identified from prospective databases maintained at 9 inst...

    journal_title:Annals of surgery

    pub_type: 杂志文章,多中心研究

    doi:10.1097/SLA.0b013e3181fb8df6

    authors: Shields CJ,Tiret E,Winter DC,International Rectal Carcinoid Study Group.

    更新日期:2010-11-01 00:00:00

  • Postconditioning, a series of brief interruptions of early reperfusion, prevents neurologic injury after spinal cord ischemia.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/01.sla.0000217608.08582.35

    authors: Jiang X,Shi E,Nakajima Y,Sato S

    更新日期:2006-07-01 00:00:00

  • Longitudinal pancreaticojejunostomy in chronic relapsing pancreatitis with onset in childhood.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-198405000-00017

    authors: Scott HW Jr,Neblett WW,O'Neill JA Jr,Sawyers JL,Avant GS,Starnes VA

    更新日期:1984-05-01 00:00:00

  • Survival outcome of local excision versus radical resection of colon or rectal carcinoma: a Surveillance, Epidemiology, and End Results (SEER) population-based study.

    abstract:OBJECTIVE:To compare cancer-specific results of local excision with major resection. BACKGROUND:Technological advances have enabled endoscopic and local excision techniques to be applied in the treatment of early colorectal cancer in preference to radical surgery. METHOD:Patients with stage 0 (carcinoma in situ) or s...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0b013e3182a4e85a

    authors: Bhangu A,Brown G,Nicholls RJ,Wong J,Darzi A,Tekkis P

    更新日期:2013-10-01 00:00:00

  • Angiopoietin-2, marker and mediator of endothelial activation with prognostic significance early after trauma?

    abstract:OBJECTIVE:To measure plasma levels of angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), and vascular endothelial growth factor (VEGF) early after trauma and to determine their clinical significance. BACKGROUND:Angiopoietins and VEGF play a central role in the physiology and pathophysiology of endothelial cells. Ang-2 ha...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0b013e318162d616

    authors: Ganter MT,Cohen MJ,Brohi K,Chesebro BB,Staudenmayer KL,Rahn P,Christiaans SC,Bir ND,Pittet JF

    更新日期:2008-02-01 00:00:00

  • Ileal pouch-anal anastomosis: comparison of results in familial adenomatous polyposis and chronic ulcerative colitis.

    abstract::The aim of this study was to compare the immediate postoperative results and the long-term outcome of ileal pouch-anal anastomosis in 94 patients with familial adenomatous polyposis to those in 758 patients with ulcerative colitis. Two colitis patients died after operation (0.3%), but no polyposis patients died. Overa...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-198909000-00002

    authors: Dozois RR,Kelly KA,Welling DR,Gordon H,Beart RW Jr,Wolff BG,Pemberton JH,Ilstrup DM

    更新日期:1989-09-01 00:00:00

  • Implications of Tumor Characteristics and Treatment Modality on Local Recurrence and Functional Outcomes in Children with Chest Wall Sarcoma: A Pediatric Surgical Oncology Research Collaborative Study.

    abstract:OBJECTIVE:To determine the impact of tumor characteristics and treatment approach on (1) local recurrence, (2) scoliosis development and (3) patient-reported quality of life in children with sarcoma of the chest wall. SUMMARY BACKGROUND DATA:Children with chest wall sarcoma require multimodal therapy including chemoth...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0000000000004579

    authors: Harris CJ,Helenowski I,Murphy AJ,Mansfield SA,LaQuaglia MP,Heaton TE,Cavalli M,Murphy JT,Newman E,Overmen RE,Kartal TT,Cooke-Barber J,Donaher A,Malek MM,Kalsi R,Kim ES,Zobel MJ,Goodhue CJ,Naik-Mathuria BJ,Jefferson

    更新日期:2020-11-04 00:00:00

  • Effects of a stable prostacyclin analog on experimental ischemic acute renal failure.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-198807000-00009

    authors: Tobimatsu M,Ueda Y,Saito S,Tsumagari T,Konomi K

    更新日期:1988-07-01 00:00:00

  • Effects of vagotomy and gastrectomy on pancreatic glucagon release.

    abstract::The effects of gastrectomy and vagotomy on pancreatic glucagon release were investigated clinically. The study included 20 men and eight women, who ranged in age from 28 to 69 years, and who were divided into the following four groups: 1) patients with gastroduodenal ulcers treated with partial gastrectomy, by the Bil...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-198107000-00005

    authors: Sudo T,Kawamura M,Umemura H,Shiraha S,Kuyama T

    更新日期:1981-07-01 00:00:00

  • Management of external gastrointestinal fistulas.

    abstract::We analyzed the course of 186 patients with external gastrointestinal fistulas treated at the University of California Medical Center, San Francisco from 1968 to 1977. There were 82 patients in the earlier group (1968-1971) and 104 patients in the later group (1972-1977). The groups differed in that 35% of patients in...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-197810000-00003

    authors: Reber HA,Roberts C,Way LW,Dunphy JE

    更新日期:1978-10-01 00:00:00

  • Hyperthermic pelvic perfusion with tumor necrosis factor-α for locally advanced cancers: encouraging results of a phase II study.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0b013e318242ebe7

    authors: Bonvalot S,de Baere T,Mendiboure J,Paci A,Farace F,Drouard-Troalen L,Bonnet L,Hakime A,Bonniaud G,Raynard B,Israel P,Le Cesne A,Eggermont AM,Laplanche A,Muret J

    更新日期:2012-02-01 00:00:00

  • Surgical management of pheochromocytoma with the use of metyrosine.

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    journal_title:Annals of surgery

    pub_type: 临床试验,杂志文章

    doi:10.1097/00000658-199011000-00010

    authors: Perry RR,Keiser HR,Norton JA,Wall RT,Robertson CN,Travis W,Pass HI,Walther MM,Linehan WM

    更新日期:1990-11-01 00:00:00

  • Treatment of intra-abdominal and abdominal wall desmoid tumors with drugs that affect the metabolism of cyclic 3',5'-adenosine monophosphate.

    abstract::A female patient with Gardner's syndrome was treated with delta1-testololactone (200 mg daily) because of growth of a large desmoid tumor in the pelvis and lower abdomen and a tumor in a scar from a previous laparotomy. There was also pain and swelling of the left leg. An immediate effect of the drug therapy was compl...

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    pub_type: 杂志文章

    doi:10.1097/00000658-197503000-00009

    authors: Waddell WR

    更新日期:1975-03-01 00:00:00

  • Predicting risk for serious complications with bariatric surgery: results from the Michigan Bariatric Surgery Collaborative.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0b013e318230058c

    authors: Finks JF,Kole KL,Yenumula PR,English WJ,Krause KR,Carlin AM,Genaw JA,Banerjee M,Birkmeyer JD,Birkmeyer NJ,Michigan Bariatric Surgery Collaborative, from the Center for Healthcare Outcomes and Policy.

    更新日期:2011-10-01 00:00:00

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    journal_title:Annals of surgery

    pub_type: 杂志文章,评审

    doi:10.1097/SLA.0b013e3181492c0d

    authors: Williams TM,Harken AH

    更新日期:2008-01-01 00:00:00

  • A prospective randomized trial comparing R1 subtotal gastrectomy with R3 total gastrectomy for antral cancer.

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    journal_title:Annals of surgery

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1097/00000658-199408000-00009

    authors: Robertson CS,Chung SC,Woods SD,Griffin SM,Raimes SA,Lau JT,Li AK

    更新日期:1994-08-01 00:00:00

  • Variation and Impact of Multiple Complications on Failure to Rescue After Inpatient Surgery.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0000000000001917

    authors: Massarweh NN,Anaya DA,Kougias P,Bakaeen FG,Awad SS,Berger DH

    更新日期:2017-07-01 00:00:00

  • Accelerated wound healing with stapled enteric suture lines. An experimental study comparing traditional sewing techniques and a stapling device.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-198503000-00019

    authors: Ballantyne GH,Burke JB,Rogers G,Lampert EG,Boccia J

    更新日期:1985-03-01 00:00:00

  • A Study of Clinical Coding Accuracy in Surgery: Implications for the Use of Administrative Big Data for Outcomes Management.

    abstract:BACKGROUND:Clinical coding is the translation of clinical activity into a coded language. Coded data drive hospital reimbursement and are used for audit and research, and benchmarking and outcomes management purposes. METHODS:We undertook a 2-center audit of coding accuracy across surgery. Clinician-auditor multidisci...

    journal_title:Annals of surgery

    pub_type: 杂志文章,多中心研究

    doi:10.1097/SLA.0000000000000851

    authors: Nouraei SA,Hudovsky A,Frampton AE,Mufti U,White NB,Wathen CG,Sandhu GS,Darzi A

    更新日期:2015-06-01 00:00:00

  • Hilar cholangiocarcinoma: current management.

    abstract:OBJECTIVE:To review the literature with regard to outcome of surgical management for hilar cholangiocarcinoma (Klatskin tumor). BACKGROUND:Hilar cholangiocarcinoma is a rare tumor with a poor prognosis. Surgical resection provides the only possibility for cure. Advances in hepatobiliary imaging and surgical strategies...

    journal_title:Annals of surgery

    pub_type: 杂志文章,评审

    doi:10.1097/SLA.0b013e3181afe0ab

    authors: Ito F,Cho CS,Rikkers LF,Weber SM

    更新日期:2009-08-01 00:00:00