Heterochromatic flicker photometry.

abstract：:Nucleotide Binding Domains (NBDs) are responsible for the ATPase activity of the multidrug resistance protein 1 (MRP1). A series of NBD1-linker-NBD2 chimeric fusion proteins were constructed, expressed and purified, and their ATPase activities were analyzed. We report here that a GST linked NBD1(642-890)-GST-NBD2(1286...

journal_title：Archives of biochemistry and biophysics

authors： Wan L,Liang X,Huang Y

更新日期：2009-05-15 00:00:00

abstract：:Functional activities of isolated atria from 4-week diabetic rats and their anoxic tolerance and recovery were compared to those of control rats. The present study showed a two-fold increase in maximum developed contraction force (CF), the rate of force development, and the rate of relaxation in isolated atria from di...

journal_title：Archives of biochemistry and biophysics

authors： Savabi F,Kirsch A

更新日期：1990-05-15 00:00:00

abstract：:Bone-forming cells originate from distinct embryological layers, mesoderm (axial and appendicular bones) and ectoderm (precursor of neural crest cells, which mainly form facial bones). These cells will develop bones by two principal mechanisms: intramembranous and endochondral ossification. In both cases, condensation...

journal_title：Archives of biochemistry and biophysics

authors： Paiva KB,Granjeiro JM

更新日期：2014-11-01 00:00:00

abstract：:A current hypothesis explaining the aging process implicates the accumulation of oxidized protein in animal tissues. This is primarily based on a series of reports showing an age-dependent increase in protein carbonyl content and an age-dependent decrease in the activities of enzymes, especially of alkaline proteases,...

journal_title：Archives of biochemistry and biophysics

authors： Cao G,Cutler RG

更新日期：1995-06-20 00:00:00

abstract：:I had the fortune to be introduced to Helmut Sies during the mid 1980s, while working as a post-doctoral scientist at the University of California, Berkeley. At that time, Helmut was a frequent visitor of the Bruce Ames' laboratory and a leading authority in antioxidants and oxidative stress. His concepts, ideas and w...

journal_title：Archives of biochemistry and biophysics

authors： Stocker R

更新日期：2016-04-01 00:00:00

abstract：:A detailed knowledge about the substrate specificities of the heparin lyases is necessary when using these enzymes as tools for elucidating the sequence of heparin and heparan sulfate. The substrate specificity of heparin lyases I, II, and III have been profiled with structurally defined, heparin-derived oligosacchari...

journal_title：Archives of biochemistry and biophysics

authors： Desai UR,Wang HM,Linhardt RJ

更新日期：1993-11-01 00:00:00

abstract：:The conformational behavior of receptor-bound acetylcholine (ACh) was investigated by molecular dynamics simulations. Based on the great similarity among muscarinic receptors, the study was focused on the human M(1), M(2), and M(5) receptors as previously modeled by us. The results showed that receptor-bound ACh was n...

journal_title：Archives of biochemistry and biophysics

authors： Vistoli G,Pedretti A,Testa B,Matucci R

更新日期：2007-08-01 00:00:00

abstract：:Fluidity of the red blood cell membrane decreases as pH changes from 8 to 7.5. In rat liver mitochondrial (RLM) membrane fluidity precipitously declines as pH drops from 7.35 toward 7.0. With dithionitrobenzoate (Nbs2), reaction rates of mitochondrial -SH groups from rat liver and heart (RHM) and in beef heart submito...

journal_title：Archives of biochemistry and biophysics

authors： Zimmer G,Freisleben HJ,Fuchs J

更新日期：1990-11-01 00:00:00

abstract：:Tobacco-induced oxidative stress leads to chronic inflammation and is implicated in the development of many human epithelial cancers, including head and neck cancer. Cigarette smoke exposure was shown to induce the expression of the ΔNp63α and nitric oxide synthase (NOS)-2 in head and neck squamous cell carcinoma cell...

journal_title：Archives of biochemistry and biophysics

authors： Ratovitski EA

更新日期：2011-02-15 00:00:00

abstract：:An attempt was made to relate the iron and phosphate content of ferritin to its subunit composition. Ferritins from various tissues were separated according to their subunit composition by anion exchange chromatography and according to their iron content by density-gradient centrifugation. Iron and phosphate contents ...

journal_title：Archives of biochemistry and biophysics

authors： Juan SH,Aust SD

更新日期：1998-09-15 00:00:00

abstract：:Recently, we synthesized and characterized vanadyl saccharides to evaluate the effects of various vanadate and vanadyl complexes, which differ in their oxidation states on various biomacromolecules and cellular activities (1, 2). Here, we report that both vanadate (+V oxidation state) and different vanadyl species (+I...

journal_title：Archives of biochemistry and biophysics

authors： Krishnamoorthy T,Sreedhara A,Rao CP,Ramaiah KV

更新日期：1998-01-01 00:00:00

abstract：:SH2 domain-containing inositol 5-phosphatase 2 (SHIP2) binds with the Y1356-phosphorylated hepatocyte growth factor (HGF) receptor, c-MET, through its SH2 domain, which is essential for the role of SHIP2 in HGF-induced cell scattering and cell spreading. Previously, the experimental structure of the SH2 domain from SH...

journal_title：Archives of biochemistry and biophysics

authors： Wang Z,Nie Y,Zhang K,Xu H,Ramelot TA,Kennedy MA,Liu M,Zhu J,Yang Y

更新日期：2018-10-15 00:00:00

abstract：:Four different monoclonal antibodies (MAbs) to human epidermal growth factor (hEGF) were obtained from mouse hybridoma cell lines. They were highly specific for hEGF and did not cross-react appreciably with mouse EGF. The immunoglobulin class of these MAbs was IgG1 or IgG2a. The apparent Kd values for hEGF of these MA...

journal_title：Archives of biochemistry and biophysics

authors： Yoshitake Y,Nishikawa K

更新日期：1988-06-01 00:00:00

abstract：:Inhibition of the catecholase and cresolase reactions of the alpha, beta, and gamma isozymes of Agaricus bisporus tyrosinase by benzoic acid was investigated at 25.0 and 8.0 degrees C at pH 5.60 in air-saturated solutions. Benzoic acid is a simple competitive inhibitor of the cresolase reaction of all three isozymes. ...

journal_title：Archives of biochemistry and biophysics

authors： Menon S,Fleck RW,Yong G,Strothkamp KG

更新日期：1990-07-01 00:00:00

abstract：:In bovine retinal pigment epithelium membranes we have found three hydrolases which were active against trans-retinyl palmitate. This was possible by assaying different subcellular fractions as a function of pH in the range 3-9. Detection of these activities has been favored by the use in the enzyme assay of Triton X-...

journal_title：Archives of biochemistry and biophysics

authors： Gueli MC,Nicotra CM,Pintaudi AM,Paganini A,Pandolfo L,De Leo G,Di Bella MA

更新日期：1991-08-01 00:00:00

abstract：:A new strategy for the functional reconstitution of membrane proteins is described. This approach introduces a new class of protein stabilizing agents--osmolytes--whose presence at high concentration (10-20%) during detergent solubilization prevents the inactivations that normally occur when proteins are extracted fro...

journal_title：Archives of biochemistry and biophysics

authors： Maloney PC,Ambudkar SV

更新日期：1989-02-15 00:00:00

abstract：:Drought is one of the most serious factors affecting crop yields in the world. Macleaya cordata (Willd.) is a draught-tolerant medicinal plant that has been proposed as a pioneer crop to be cultivated in arid areas. However, the exact molecular mechanisms through which M. cordata responds to draught stress remain elus...

journal_title：Archives of biochemistry and biophysics

authors： Yu L,Zhou L,Liu W,Huang P,Jiang R,Tang Z,Cheng P,Zeng J

更新日期：2020-05-15 00:00:00

abstract：:In cells expressing only the Glut1 isoform of glucose transporters, we have shown that glucose transport is markedly stimulated in response to hypoxia or inhibition of oxidative phosphorylation, conditions that would be expected to cause a stimulation of AMP-activated protein kinase (AMPK) activity. In the present stu...

journal_title：Archives of biochemistry and biophysics

authors： Abbud W,Habinowski S,Zhang JZ,Kendrew J,Elkairi FS,Kemp BE,Witters LA,Ismail-Beigi F

更新日期：2000-08-15 00:00:00

abstract：:CYP27A1, an enzyme with several important roles in cholesterol homeostasis and vitamin D₃ metabolism, has been ascribed anti-atherogenic properties. This study addresses an important problem regarding how this enzyme, involved in cholesterol metabolism in the liver and peripheral tissues, is regulated. Our results ide...

journal_title：Archives of biochemistry and biophysics

authors： Norlin M,Pettersson H,Tang W,Wikvall K

更新日期：2011-02-15 00:00:00

abstract：:Sarcolemmal vesicles of white and red skeletal muscles of the rabbit were prepared by consecutive density gradient centrifugations in sucrose and dextran according to Seiler and Fleischer (1982, J. Biol. Chem. 257, 13,862-13,871). White and red muscle membrane fractions enriched in sarcolemma were characterized by hig...

journal_title：Archives of biochemistry and biophysics

authors： Wetzel P,Gros G

更新日期：1990-06-01 00:00:00

abstract：:The majority of hypertrophic cardiomyopathy mutations in (cTnT) occur within the alpha-helical tropomyosin binding TNT1 domain. A highly charged region at the C-terminal end of TNT1 unwinds to create a flexible "hinge". While this region has not been structurally resolved, it likely acts as an extended linker between ...

journal_title：Archives of biochemistry and biophysics

authors： Moore RK,Abdullah S,Tardiff JC

更新日期：2014-06-15 00:00:00

abstract：:Sesquiterpene cyclases (synthases) catalyze the conversion of the isoprenoid intermediate farnesyl diphosphate to various sesquiterpene structural types. In plants, many sesquiterpenes are produced as defensive chemicals (phytoalexins) or mediators of chemical communication (i.e., pollinator attractants). A number of ...

journal_title：Archives of biochemistry and biophysics

authors： Mercke P,Crock J,Croteau R,Brodelius PE

更新日期：1999-09-15 00:00:00

abstract：:The effects of in vitro addition of halogenated hydrocarbons on the susceptibility of various rat tissues to lipid peroxidation, and of iron overload and dietary vitamin E in the intact rat on subsequent lipid peroxidation in rat tissue slices were examined. The ease and speed of tissue slice preparation allowed testi...

journal_title：Archives of biochemistry and biophysics

authors： Gavino VC,Dillard CJ,Tappel AL

更新日期：1984-09-01 00:00:00

abstract：:Hyaluronidase from fresh human serum was purified to apparent homogeneity in a two-step procedure. Potent serum inhibitors of hyaluronidase activity were removed during the course of the purification. Isolation of the enzyme was expedited by the use of a newly devised ELISA-like assay. Enzyme activity was measured by ...

journal_title：Archives of biochemistry and biophysics

authors： Afify AM,Stern M,Guntenhöner M,Stern R

更新日期：1993-09-01 00:00:00

abstract：:Homogeneous preparations of fructose-1,6-bisphosphatase from mouse, man, rabbit, pig, and rat were tested as substrates for cyclic AMP-dependent protein kinase. Up to 1 mol of [32P]phosphate per mole enzyme subunit was incorporated into fructose-1,6-bisphosphatase from pig and rabbit liver, which should be compared wi...

journal_title：Archives of biochemistry and biophysics

authors： Ekdahl KN

更新日期：1988-04-01 00:00:00

abstract：:Reports suggest that microRNAs have implications in the development of several diseases including cancer. It is therefore believed that miRs may act as therapeutic targets for cancer treatment. The treatment of ovarian cancer is mainly obstructed by lack of biomarkers and efficient drug targets. Against this backdrop,...

journal_title：Archives of biochemistry and biophysics

authors： Si L,Jia Y,Lin R,Jian W,Yu Q,Yang S

更新日期：2019-06-15 00:00:00

abstract：:Metallothionein isoform II was purified from calf liver. The protein had a metal content of 1.2-1.9 Cu ions and 5.6-6.2 Zn ions per molecule in different preparations. The complete amino acid sequence of the molecule was determined by automatic Edman degradation of CNBr and tryptic peptides of the carboxymethylated pr...

journal_title：Archives of biochemistry and biophysics

authors： Winge DR,Gray WR,Zelazowski A,Garvey JS

更新日期：1986-02-15 00:00:00

abstract：:The major proteins to which 3-methylcholanthrene specifically binds have been purified over 480-fold with a 45% yield compared to a rat liver 100,000g supernate. The procedure involved a batch ion-exchange technique together with hydrophobic gel filtration and chromatofocusing chromatography. The multiple, specific 3-...

journal_title：Archives of biochemistry and biophysics

authors： Tierney B,Munzer S,Bresnick E

更新日期：1983-09-01 00:00:00

abstract：:The aim of this study was to investigate the effect of insulin resistance on glycogen concentration and glycogen synthase activity in the red and white gastrocnemius muscles and to determine whether the inverse relationship existing between glycogen concentration and enzyme activity is maintained in insulin resistant ...

journal_title：Archives of biochemistry and biophysics

authors： Coderre L,Vallega GA,Pilch PF,Chipkin SR

更新日期：2007-08-01 00:00:00

abstract：:A microsomal preparation from seedlings of Zea mays catalyzed the NAD+-dependent oxidative decarboxylation of several substrates, including 4alpha-carboxy-cholest-7-en-3beta-ol, synthesized according to a new procedure, giving the first in vitro evidence for this enzymatic activity in a higher plant. A GC assay has be...

journal_title：Archives of biochemistry and biophysics

authors： Rondet S,Taton M,Rahier A

更新日期：1999-06-15 00:00:00