Expression of cALLa/NEP on gliomas: a possible marker of malignancy.

Abstract:

:First described on pre-B leukemia cells, the common acute lymphoblastic leukemia antigen (cALLa) is also expressed on glioma cells in vitro. Its identity to neutral endopeptidase (NEP) (E.C.3.24.11) was corroborated by our finding that cALLa positive glioma cells had NEP activity. To study cALLa/NEP distribution on glial tumours in vivo, we examined 76 brain tumour biopsies by immunostaining techniques on frozen tissue sections using anti-cALLa (FAH99) and anti-NEP (135 A 3) monoclonal antibodies. We found that 96% of grade 4 gliomas (25/26) expressed NEP. Whereas only 45% (4/9) of grade 3 or anaplastic astrocytomas did. In low grade gliomas, we found 2 positive tumours out of 21 tested (10%). Double immunostaining procedures revealed that NEP was co-expressed with GFAP. However no NEP could be detected on non-glial brain tumours nor on reactive astrocytes. These results suggest that cALLa/NEP expression could be linked to malignant progression of gliomas.

journal_name

Acta Neurochir (Wien)

journal_title

Acta neurochirurgica

authors

Monod L,Hamou MF,Ronco P,Verroust P,de Tribolet N

doi

10.1007/BF01401105

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

3-7

issue

1-2

eissn

0001-6268

issn

0942-0940

journal_volume

114

pub_type

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