Genotyping of rifampin-resistant Mycobacterium tuberculosis isolates from western Turkey.

Abstract:

BACKGROUND:Although the rate of multiple drug resistance is high, there is no published data on the transmission rate of drug-resistant strains of Mycobacterium tuberculosis in the Aegean region of western Turkey that are based on molecular methods. METHODS:IS6110 and pTBN12 restriction fragment length polymorphism (RFLP) methods were used for typing M. tuberculosis strains isolated from 26 sputum samples from 26 patients. RESULTS:Nineteen of the rifampin-resistant isolates (73.1%) contained 6 to 11 copies of IS6110. Eighteen different IS6110 DNA fingerprint patterns were observed in the 26 rifampin-resistant isolates. Twenty-three of the 26 rifampin-resistant isolates were also resistant to isoniazid. When evaluated together, both methods yielded 21 (80.9%) different banding patterns and the level of clustering was 34.6%. The average number per pattern was 1.23 (26/21). CONCLUSIONS:IS6110 fingerprinting suggests that the rifampin-resistant isolates obtained from the Aegean region had a relatively high clustering rate and were clonally related. These findings showed that the rifampin-resistant isolates are actively transmitted between patients. Urgent measures should be taken to prevent the spread of these resistant strains.

journal_name

Ann Saudi Med

journal_title

Annals of Saudi medicine

authors

Cavusoglu C,Durmaz R,Bilgic A,Gunal S

doi

10.5144/0256-4947.2004.102

subject

Has Abstract

pub_date

2004-03-01 00:00:00

pages

102-5

issue

2

eissn

0256-4947

issn

0975-4466

journal_volume

24

pub_type

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