Proteolytic, hemorrhagic, and neurotoxic activities caused by Leptodeira annulata ashmeadii (Serpentes: Colubridae) Duvernoy's gland secretion.

Abstract:

OBJECTIVE:The main goal was to explore the different toxin properties (proteolytic, hemorrhagic, and neurotoxic) of Leptodeira annulata ashmeadii Duvernoy's gland secretion (DGS). METHODS:To separate and characterize the different proteins present in L. annulata ashmeadii DGS, 20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was run. To partially purify the proteolytic activity, Mono Q2 column ion-exchange chromatography was used. Hemorrhagic activity was analyzed in chicken embryos and mice (skin and peritoneum). Neurotoxic disorders were analyzed in mice inoculated with L. annulata ashmeadii DGS. RESULTS:The approximate relative mass of 21 protein bands was determined using the Multi-Analyst PC version 1.1 (Bio-Rad) program. L. annulata ashmeadii DGS proved to have proteolytic, hemorrhagic, and neurotoxic activities. CONCLUSIONS:Given the properties of the secretions of its Duvernoy's gland, L. annulata ashmeadii should be added to the list of venomous colubrids.

journal_name

Wilderness Environ Med

authors

Lemoine K,Girón ME,Aguilar I,Navarrete LF,Rodríguez-Acosta A

doi

10.1580/1080-6032(2004)015[0082:phanac]2.0.co;2

subject

Has Abstract

pub_date

2004-07-01 00:00:00

pages

82-9

issue

2

eissn

1080-6032

issn

1545-1534

pii

S1080-6032(04)70451-2

journal_volume

15

pub_type

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