Diagnosis of New World leishmaniasis: specific detection of species of the Leishmania braziliensis complex by amplification of kinetoplast DNA.

Abstract:

:We have sequenced single kinetoplast DNA minicircles from three species and part of a minicircle from the fourth major species within the Leishmania braziliensis complex. Alignment of these sequences with each other and with those of other kinetoplastids allowed the selection of a pair of oligonucleotides suitable as primers in a polymerase chain reaction which is highly specific for the Leishmania braziliensis complex. The reaction is capable of detecting less than one femtogramme of kinetoplast DNA. It has been tested with crude specimens from South American leishmaniasis patients, potential wild animal reservoirs and sandfly vectors. The tests indicate that these primers are suitable for diagnosis of leishmaniasis and potentially useful in epidemiological surveys.

journal_name

Acta Trop

journal_title

Acta tropica

authors

de Bruijn MH,Barker DC

doi

10.1016/0001-706x(92)90006-j

subject

Has Abstract

pub_date

1992-09-01 00:00:00

pages

45-58

issue

1

eissn

0001-706X

issn

1873-6254

pii

0001-706X(92)90006-J

journal_volume

52

pub_type

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