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Evidence that the loss of rat liver cytochrome P450 in vitro is not solely associated with the use of collagenase, the loss of cell-cell contacts and/or the absence of an extracellular matrix.

Abstract：

:Two methods avoiding the widespread technique of collagenase perfusion have been employed to study the regulation of total cytochrome P450 content in rat hepatocyte culture. One technique required the perfusion of the liver with the chelating agent EDTA to dissociate the parenchymal cells prior to culture. Over a period of 48 hr, cultured hepatocytes isolated by EDTA perfusion showed comparable losses of cytochrome P450 as cells isolated by perfusion with collagenase. The second technique involved the culture of 210-240 microns thick "precision cut" liver slices. The results presented here indicate that the liver slices remain viable for 24 hr of culture, but that liver slices also lose their cytochrome P450 content at a comparable rate to collagenase prepared cells in culture. Collectively the results suggest that there is not a direct causal relationship between the loss of cytochrome P450 and one or a combination of the use of collagenase; the loss of cell-cell contacts and the absence of an extracellular matrix.

journal_name

Biochem Pharmacol

journal_title

Biochemical pharmacology

authors

Wright MC,Paine AJ

doi

10.1016/0006-2952(92)90283-o

subject

Has Abstract

pub_date

1992-01-22 00:00:00

pages

237-43

issue

2

eissn

0006-2952

issn

1873-2968

pii

0006-2952(92)90283-O

journal_volume

43

pub_type

杂志文章

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