Abstract:
:Severe elevation of red blood cell number is often associated with hypertension and thromboembolism resulting in severe cardiovascular complications. However, some individuals such as high altitude dwellers cope well with an increased hematocrit level. We analyzed adaptive mechanisms to excessive erythrocytosis in our transgenic (tg) mice that, due to hypoxia-independent erythropoietin (Epo) overexpression, reached hematocrit values of 0.8 to 0.9 without alteration of blood pressure, heart rate, or cardiac output. Extramedullar erythropoiesis occurred in the tg spleen, leading to splenomegaly. Upon splenectomy, hematocrit values in tg mice decreased from 0.89 to 0.62. Tg mice showed doubled reticulocyte counts and an increased mean corpuscular volume. In tg mice, plasma volume was not elevated whereas blood volume was up to 25% of the body weight compared with 8% in wild-type (wt) siblings. Although plasma viscosity did not differ between tg and wt mice, tg whole-blood viscosity increased to a lower degree (4-fold) than expected from corresponding hemoconcentrated wt blood (8-fold). This moderate increase in viscosity is explicable by the up to 3-fold higher elongation of tg erythrocytes at physiologic shear rates. Apart from the nitric oxide-mediated vasodilation we reported earlier, adaptation to high hematocrit levels in tg mice involves regulated elevation of blood viscosity by increasing erythrocyte flexibility.
journal_name
Bloodjournal_title
Bloodauthors
Vogel J,Kiessling I,Heinicke K,Stallmach T,Ossent P,Vogel O,Aulmann M,Frietsch T,Schmid-Schönbein H,Kuschinsky W,Gassmann Mdoi
10.1182/blood-2003-01-0283subject
Has Abstractpub_date
2003-09-15 00:00:00pages
2278-84issue
6eissn
0006-4971issn
1528-0020pii
2003-01-0283journal_volume
102pub_type
杂志文章相关文献
BLOOD文献大全abstract::Tissue factor pathway inhibitor (TFPI) is a serine protease inhibitor of the extrinsic coagulation system, synthesized in endothelial cells, which has recently been shown to play an important role in the regulation of activated coagulation factors at the endothelial cell surface. In the present study we investigated t...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::Aberrant megakaryopoiesis is a hallmark of the myeloproliferative neoplasms (MPNs), a group of clonal haematological malignancies originating from haematopoietic stem cells, leading to an increase in mature blood cells in the peripheral blood. Sialylated derivatives of the glycan structure β4-N-acetyllactosamine (Galβ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2020007265
更新日期:2020-11-25 00:00:00
abstract::A study in 121 infants with severe combined immunodeficiency (SCID) was performed to determine the prevalence of an engraftment by transplacentally acquired maternal T cells and to explore clinical and immunological findings related to this abnormality. Each newly diagnosed patient with SCID presenting with circulatin...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.6.1847
更新日期:2001-09-15 00:00:00
abstract::Krüppel-like factor 5 (Klf5) encodes a zinc-finger transcription factor and has been reported to be a direct target of C/EBPα, a master transcription factor critical for formation of granulocyte-macrophage progenitors (GMP) and leukemic GMP. Using an in vivo hematopoietic-specific gene ablation model, we demonstrate t...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-12-684514
更新日期:2016-07-07 00:00:00
abstract::A postulated role of the contact system in anaphylactic reactions to insect stings was investigated. During prospective, in-hospital sting challenge, we collected serial blood samples from five normal volunteers and 16 patients with a history of insect-sting anaphylaxis. Activation of the contact system was assessed b...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-09-15 00:00:00
abstract::A qualitative defect of antithrombin III (AT III) has been demonstrated over three generations in eight members of an Italian family by the discrepancy between a normal amount of antigen and decreased antithrombin and anti-Xa activity in the presence or in the absence of heparin. By two-dimensional immunoelectrophores...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
abstract::Clonal expansion of activated T cells is controlled by homeostatic mechanisms leading to cell death of a large proportion of the cells. The CD3/TcR pathway induces cell death, mostly when triggered in the absence of costimulatory signal. The unique T cell-specific chemokine of the C class, lymphotactin (Lptn), has rec...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.8.2205
更新日期:2001-04-15 00:00:00
abstract::Gaucher disease, caused by a deficiency of the lysosomal enzyme glucocerebrosidase, leads to prominent glucosylceramide accumulation in lysosomes of tissue macrophages (Gaucher cells). Here we show glucosylsphingosine, the deacylated form of glucosylceramide, to be markedly increased in plasma of symptomatic nonneuron...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-05-352971
更新日期:2011-10-20 00:00:00
abstract::Human blood contains 2 populations of dendritic cells (DCs): plasmacytoid and myeloid (mDC). mDCs are subdivided into 3 subsets using the surface markers CD16, CD1c, and BDCA-3. Their role as pathogen sentinels and adjuvant targets was tested by phenotypic and functional analysis. We show that mDC subsets are immature...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-08-038422
更新日期:2007-06-15 00:00:00
abstract::Serum response factor (SRF) is a ubiquitously expressed transcription factor and master regulator of the actin cytoskeleton. We have previously shown that SRF is essential for megakaryocyte maturation and platelet formation and function. Here we elucidate the role of SRF in neutrophils, the primary defense against inf...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-06-507582
更新日期:2014-05-08 00:00:00
abstract::Proteolytic events at the cell surface are essential in the regulation of signal transduction pathways. During the past years, the family of type II transmembrane serine proteases (TTSPs) has acquired an increasing relevance because of their privileged localization at the cell surface, although our current understandi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-04-149773
更新日期:2008-09-15 00:00:00
abstract::Mutations that produce glucose-6-phosphate dehydrogenase (G6PD) deficiency have been identified in samples from patients with hemolytic disease in the United States, and in G6PD-deficient samples from Greece, the Canary Islands, the Czech and Slovak Republics, South China, and in samples from the Coriell Cell Reposito...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::HLA-G is a major histocompatibility complex class Ib molecule whose constitutive tissue distribution is restricted mainly to trophoblast cells at the maternal-fetal interface during pregnancy. In this study, we demonstrated the ability of the soluble HLA-G1 (sHLA-G1) isoform to inhibit fibroblast growth factor-2 (FGF2...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-12-019919
更新日期:2006-10-15 00:00:00
abstract::Patients referred to tertiary care centers occasionally may have their diagnostic procedures repeated and have a final diagnosis that differs from that of the referring center. The aim of this study was to evaluate discordance rates and their clinical implications in the diagnosis of patients with myelodysplastic synd...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-03-342642
更新日期:2011-10-27 00:00:00
abstract::The therapeutic potential of hematopoietic stem cell (HSC) gene therapy can be fully exploited only by reaching efficient gene transfer into HSCs without compromising their biologic properties. Although HSCs can be transduced by HIV-derived lentiviral vectors (LVs) in short ex vivo culture, they display low permissivi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-10-4047
更新日期:2006-06-01 00:00:00
abstract::Plasmin exposure modulates platelet aggregation responses, but a direct effect of plasmin on the platelet fibrinogen receptor, glycoprotein IIb/IIIa (GPIIb/IIIa), has never been conclusively shown in a plasma milieu. To examine this issue, we incubated platelets in platelet-rich plasma with plasmin and measured the ef...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-01-15 00:00:00
abstract::Fibrinogen, coagulation factor VII (FVII), and factor VIII (FVIII) and its carrier von Willebrand factor (vWF) play key roles in hemostasis. Previously identified common variants explain only a small fraction of the trait heritabilities, and additional variations may be explained by associations with rarer variants wi...
journal_title:Blood
pub_type: 杂志文章,meta分析
doi:10.1182/blood-2015-02-624551
更新日期:2015-09-10 00:00:00
abstract::Investigators in the United Kingdom have shown that hereditary amyloidosis can be misdiagnosed as Ig light-chain (AL) amyloidosis because family history is an ineffective screen, and tissue staining used to type amyloid is unreliable. Misdiagnosis of AL can lead to inappropriate use of chemotherapy and failure to diag...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-10-4148
更新日期:2006-05-01 00:00:00
abstract::Under evolutionary pressure to counter the toxicity of iron and to maintain adequate iron supply for hemoglobin synthesis and essential metabolic functions, humans and other vertebrates have effective mechanisms to conserve iron and to regulate its concentration, storage, and distribution in tissues. The iron-regulato...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2011-01-258467
更新日期:2011-04-28 00:00:00
abstract::We compared urokinase-type plasminogen activator (u-PA) in fluid phase and u-PA bound with its receptor on human blood monocytes with respect to proteolytic activity and susceptibility to inactivation by the plasminogen activator inhibitors PAI-1 and PAI-2. Receptor-bound u-PA is catalytically twice as efficient as fl...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-09-01 00:00:00
abstract::The number of CD34+ cells in the peripheral blood of cancer patients is known to be increased following the administration of high dose chemotherapy and hematopoietic growth factors. These so-called peripheral blood stem cell grafts are now frequently used for autologous transplantation of patients with malignancies. ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-01-15 00:00:00
abstract::Interaction between human platelets and bacterial endotoxin was studied in vitro with transmission and scanning electron microscopy. Washed human platelets, whose aggregation was blocked with apyrase, were incubated in a plasma-free medium containing crude endotoxin that had previously been complexed with copper. Thir...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1978-03-01 00:00:00
abstract::Short-chain fatty acids (SCFAs) and dimethyl sulfoxide (DMSO) induce adult erythroid differentiation in murine erythroleukemia (MEL) cells, but only SCFAs concurrently up-regulate expression from the endogenous embryonic globin gene epsilony. The epsilony promoter, linked to a reporter gene and stably transfected into...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-12-3766
更新日期:2003-12-01 00:00:00
abstract::All-trans retinoic acid (tRA) and arsenic trioxide (As(2)O(3)) induce non-cross-resistant complete clinical remission in patients with acute promyelocytic leukemia with t(15;17) translocation and target PML-RARalpha, the leukemogenic protein, by different pathways suggesting a possible therapeutic synergism. To evalua...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.1.264
更新日期:2001-01-01 00:00:00
abstract::In common variable immunodeficiency (CVID) defects in early stages of B-cell development, bone marrow (BM) plasma cells and T lymphocytes have not been studied systematically. Here we report the first morphologic and flow cytometric study of B- and T-cell populations in CVID BM biopsies and aspirates. Whereas the hema...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-11-321695
更新日期:2011-07-14 00:00:00
abstract::Severe congenital neutropenia (CN) is a preleukemic bone marrow failure syndrome with a 20% risk of evolving into leukemia or myelodysplastic syndrome (MDS). Patterns of acquisition of leukemia-associated mutations were investigated using next-generation deep-sequencing in 31 CN patients who developed leukemia or MDS....
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-11-538025
更新日期:2014-04-03 00:00:00
abstract::Glanzmann thrombasthenia is an autosomal recessive disorder of the platelet glycoproteins (GP) IIb and IIIa. These glycoproteins normally serve as receptors for other adhesive glycoproteins, including fibrinogen, von Willebrand factor, and fibronectin. Most patients affected by Glanzmann thrombasthenia have low levels...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::Circulating monocytes can differentiate into dendritic cells (moDCs), which are potent inducers of adaptive immune responses. Previous reports show that granulocyte macrophage-colony-stimulating factor (GM-CSF) and interleukin-4 induce monocyte differentiation into moDCs in vitro, but little is known about the physiol...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-02-076364
更新日期:2007-10-01 00:00:00
abstract::Previous studies suggested a role for prostaglandins or thromboxane A2, or both in the exposure of fibrinogen receptors on normal platelets in response to several aggregating agents. Platelets from diabetics are known to be more sensitive to aggregating agents and to produce more prostaglandins and thromboxane than pl...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::Mutations in a recently identified gene HJV (also called HFE2, or repulsive guidance molecule C, RgmC) are the major cause of juvenile hemochromatosis (JH). The protein product of HJV, hemojuvelin, contains a C-terminal glycosylphosphatidylinositol anchor, suggesting that it can be present in either a soluble or a cel...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-05-1845
更新日期:2005-10-15 00:00:00