Abstract：
:beta-Glucosidase activity [EC 3.2.1.21] was measured in the salivary glands and the gut of wood-eating termite, Neotermes koshunensis (Shiraki). 75% of the activity was detected in the salivary glands, whereas 15% of the activity was present in the hindgut, where numerous symbiotic flagellates reside. The salivary beta-glucosidase was partially purified by ion-exchange and gel filtration chromatography. The molecular weight of the salivary beta-glucosidase was 60 kDa, and the K(m) value on cellobiose was 2.5 mM. Its optimal pH was 5.6 and the activity was stable from 20 degrees C up to 45 degrees C. In addition to cellobiose, p-nitrophenyl-beta-D-fucopyranoside and laminaribiose were efficiently hydrolyzed by the salivary beta-glucosidase. Degenerate PCR using primers designed from N-terminal amino acid sequences of the salivary beta-glucosidase resulted in a cDNA fragment of 1730 bp, encoding 498 amino acids and with sequence similarity to glycosyl hydrolase family 1. Reverse-transcription (RT)-PCR showed that this beta-glucosidase is produced only in the salivary glands.
journal_name
Insect Biochem Mol Bioljournal_title
Insect biochemistry and molecular biologyauthors
Tokuda G,Saito H,Watanabe Hdoi
10.1016/s0965-1748(02)00108-xsubject
Has Abstractpub_date
2002-12-01 00:00:00pages
1681-9issue
12eissn
0965-1748issn
1879-0240pii
S096517480200108Xjournal_volume
32pub_type
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journal_title：Insect biochemistry and molecular biology
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journal_title：Insect biochemistry and molecular biology
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journal_title：Insect biochemistry and molecular biology
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更新日期：2005-06-01 00:00:00
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更新日期：2006-02-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2004.08.008
更新日期：2004-12-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(02)00051-6
更新日期：2002-09-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2004.03.001
更新日期：2004-05-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(96)00064-1
更新日期：1996-09-01 00:00:00
abstract：:Acetylcholinesterase (AChE) from two-spotted spider mites, Tetranychus urticae was compared between an organophosphate susceptible (TKD) and a resistant (NCN) strain. The AChE of TKD had lower affinity to acetylthiocholine and propionylthiocholine than that of NCN, and the inhibition of AChE by DDVP, ambenonium, eseri...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(03)00025-0
更新日期：2003-05-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2016.07.002
更新日期：2016-09-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(98)00019-8
更新日期：1998-07-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2008-03-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
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更新日期：2016-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：2006-04-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：1998-12-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2012-05-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2012-09-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2012.03.004
更新日期：2012-07-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2016.07.009
更新日期：2016-10-01 00:00:00
abstract：:The midgut of hematophagous insects is the initial site of infection by arthropod-borne viruses (arboviruses) and plays a crucial role in vector competence. To further understand processes that occur in the midgut in response to infection by an arbovirus, DNA microarrays were used to analyze gene expression changes fo...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2005.07.006
更新日期：2005-11-01 00:00:00
abstract：:To analyze cecropin B promoter (P-CecB) activity in vivo, we constructed transgenic silkworms that expressed EGFP under the control of P-CecB using the piggyBac transposable element. Genomic Southern blot analysis of the G1 and G2 generations indicated the stable insertion of EGFP in the genome. Injection of Escherich...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2006.03.002
更新日期：2006-05-01 00:00:00
