当前位置： SCI文献检索 > BRITISH JOURNAL OF HAEMATOLOGY期刊下所有文献 > Protein kinase C-zeta overexpression induces erythroid phenotype in the monocytic leukaemia cell line U937.

Protein kinase C-zeta overexpression induces erythroid phenotype in the monocytic leukaemia cell line U937.

Abstract：

:Previous studies have established that protein kinase C-zeta (PKC-zeta) is critical for neuronal cell differentiation. However, the role of PKC-zeta in haematopoietic cell differentiation is less clear. In this study, we have investigated the influence of PKC-zeta overexpression on the phenotype of the human monocytic U937 leukaemic cells. In two PKC-zeta-overexpressing clones (U937 zetaJ and U937 zetaB), PKC-zeta expression levels and activity were three to fourfold higher, and the enzyme accumulated both in the cytoplasm and in the nucleus compared with U937 control cells. PKC-zeta-overexpressing U937 cells exhibited an erythroid phenotype characterized by high levels of glycophorin A, cell haemoglobinization, increased GATA-1 transcripts and protein expression, compared with controls. Immunoprecipitation studies revealed that GATA-1 protein was constitutively phosphorylated in PKC-zeta-overexpressing cells. Moreover, GATA-1 did not interact with PKC-zeta but interacted with ERK1, which was constitutively activated and accumulated in the nucleus of U937 zetaJ. However, ERK1 phosphorylation inhibition by PD098059 did not influence either GATA-1 phosphorylation or GATA-1/ERK1 interaction. Collectively, these results suggest a model in which PKC-zeta induces MEK-dependent ERK1 activation, ERK1 translocation to the nucleus, GATA-1/ERK1 interaction and ERK1-independent GATA-1 phosphorylation resulting in GATA-1 accumulation. To conclude, this study provides evidence for the role of PKC-zeta in erythroid gene regulation.

journal_name

Br J Haematol

journal_title

British journal of haematology

authors

Mansat-De Mas V,de Thonel A,Gaulin V,Demur C,Laurent G,Quillet-Mary A

doi

10.1046/j.1365-2141.2002.03625.x

subject

Has Abstract

pub_date

2002-08-01 00:00:00

pages

646-53

issue

2

eissn

0007-1048

issn

1365-2141

pii

3625

journal_volume

118

pub_type

杂志文章

相关文献

BRITISH JOURNAL OF HAEMATOLOGY文献大全
  • Globin gene mapping in normal Hb A2 types of beta-thalassaemia.

    abstract：:Globin-gene mapping of DNA from 13 families with normal Hb A2 beta-thalassaemia (both type 1 and type 2) failed to detect any difference from normal in their globin-gene arrangement. We conclude that deletions such as those responsible for gamma beta-thalassaemia or a 'silent' Hb Lepore are not responsible for this ty...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1982.tb07289.x

    authors： Kanavakis E,Metaxotou-Mavromati A,Kattamis C,Aksoy M,Weatherall DJ,Wood WG

    更新日期：1982-05-01 00:00:00

  • A nonsense mutation in the GPIIb heavy chain (Ser 870-->stop) impairs platelet GPIIb-IIIa expression.

    abstract：:Glanzmann thrombasthenia (GT) is a rare autosomal recessive bleeding disorder, caused by a quantitative or qualitative defect of the GPIIb-IIIa integrin (alpha IIb beta 3), which functions as the platelet fibrinogen receptor. We report a case of type I GT due to a homozygous mutation resulting in Ser 870 to stop codon...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2141.1996.d01-1903.x

    authors： Vinciguerra C,Khelif A,Alemany M,Morle F,Grenier C,Uzan G,Gulino D,Dechavanne M,Negrier C

    更新日期：1996-11-01 00:00:00

  • CD52-negative T cells predict acute graft-versus-host disease after an alemtuzumab-based conditioning regimen.

    abstract：:Allogeneic haematopoietic stem cell transplantation (HSCT) after a reduced-intensity conditioning (RIC) regimen with fludarabine, melphalan and alemtuzmab is an effective therapy for haematological malignancies. Alemtuzumab, a monoclonal antibody against CD52, a glycosylphosphatidylinositol-anchor-bound surface protei...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/bjh.16706

    authors： Woelfinger P,Epp K,Schaefer L,Kriege D,Theobald M,Bopp T,Wagner-Drouet EM

    更新日期：2020-10-01 00:00:00

  • Isolation and culture of endothelial cells from human bone marrow.

    abstract：:Adhesive interactions between haemopoietic progenitor cells and bone marrow sinusoidal endothelium are potentially important in the homing of these cells back to the extravascular compartment of the marrow to re-establish haemopoiesis following stem cell transplantation. A simple method for the isolation and culture o...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1994.tb05128.x

    authors： Masek LC,Sweetenham JW

    更新日期：1994-12-01 00:00:00

  • Chromosomal aberrations are shared by malignant plasma cells and a small fraction of circulating CD19+ cells in patients with myeloma and monoclonal gammopathy of undetermined significance.

    abstract：:In the present study, we aimed to identify distinct structural and numerical chromosomal aberrations in peripheral blood B cells of patients with myeloma and monoclonal gammopathy of undetermined significance (MGUS), which reflect changes thought to occur at different stages of the disease process. Peripheral blood fr...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2141.2002.03529.x

    authors： Zojer N,Schuster-Kolbe J,Assmann I,Ackermann J,Strasser K,Hübl W,Drach J,Ludwig H

    更新日期：2002-06-01 00:00:00

  • Managing multiple myeloma: the emerging role of novel therapies and adapting combination treatment for higher risk settings.

    abstract：:Novel therapies have transformed the treatment paradigm for multiple myeloma with significant improvements in survival now seen in both younger and older patients. Nonetheless, the disease is heterogeneous and high-risk patients in particular continue to have poor outcome. Moreover, the disease remains incurable. Effo...

    journal_title：British journal of haematology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2141.2011.08791.x

    authors： Richardson PG,Laubach J,Mitsiades CS,Schlossman R,Hideshima T,Redman K,Chauhan D,Ghobrial IM,Munshi N,Anderson KC

    更新日期：2011-09-01 00:00:00

  • Novel interactions between UFH and TFPI in children.

    abstract：:The impact of age upon therapeutic response to unfractionated heparin (UFH) in children is proposed to reflect quantitative and potentially qualitative differences in coagulation proteins across childhood. This study explores the UFH-dependent tissue factor pathway inhibitor (TFPI) release in children compared to prev...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.2010.08373.x

    authors： Newall F,Johnston L,Summerhayes R,Lane G,Cranswick N,Monagle P,Ignjatovic V

    更新日期：2010-11-01 00:00:00

  • Inactivation of the retinoblastoma gene appears to be very uncommon in myelodysplastic syndromes.

    abstract：:Rearrangements of the retinoblastoma (RB) gene have been reported in a few cases of myelodysplastic syndromes (MDS). In addition, low or absent expression of the RB protein is found in 20-30% of cases of acute myeloid leukaemias (AML), particularly in AML with a monocytic component (M4 or M5). We performed Southern bl...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1994.tb04871.x

    authors： Preudhomme C,Vachee A,Lepelley P,Vanrumbeke M,Zandecki M,Quesnel B,Cosson A,Fenaux P

    更新日期：1994-05-01 00:00:00

  • Studies on immunological assay of vitamin K dependent factors. II. Comparison of four immunoassay methods with functional activity of protein C in human plasma.

    abstract：:We describe and compare five assay systems for Protein C (PC) in human plasma; a functional assay for PC activity, Laurell electroimmunoassay with EDTA or calcium (EDTA-Laurell or Ca-Laurell), radioimmunoassay (RIA) and immunoradiometric assay (IRMA). The lower limit of sensitivity of PC in normal reference plasma was...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1986.tb02915.x

    authors： Mikami S,Tuddenham EG

    更新日期：1986-01-01 00:00:00

  • Immunoreactive prolyl hydroxylase in patients with primary and secondary myelofibrosis.

    abstract：:Prolyl hydroxylase (PH) is an important enzyme in collagen synthesis. It is required for the hydroxylation of prolyl residues in peptide chains in collagen synthesis. Serum PH activity was measured in patients with primary myelofibrosis (agnogenic myeloid metaplasia and myelofibrosis with prior history of polycythaemi...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1987.tb02260.x

    authors： Wang JC,Wong C,Kao WW

    更新日期：1987-02-01 00:00:00

  • Histopathology, cell proliferation indices and clinical outcome in 304 patients with mantle cell lymphoma (MCL): a clinicopathological study from the European MCL Network.

    abstract：:Mantle cell lymphoma (MCL) is a distinct lymphoma subtype with a particularly poor clinical outcome. The clinical relevance of the morphological characteristics of these tumours remains uncertain. The European MCL Network reviewed 304 cases of MCL to determine the prognostic significance of histopathological character...

    journal_title：British journal of haematology

    pub_type： 杂志文章,多中心研究

    doi：10.1111/j.1365-2141.2005.05716.x

    authors： Tiemann M,Schrader C,Klapper W,Dreyling MH,Campo E,Norton A,Berger F,Kluin P,Ott G,Pileri S,Pedrinis E,Feller AC,Merz H,Janssen D,Hansmann ML,Krieken H,Möller P,Stein H,Unterhalt M,Hiddemann W,Parwaresch R,Europ

    更新日期：2005-10-01 00:00:00

  • Rh immunization facilitated by passively-administered anti-Rh?

    abstract：:The response to 0.8 ml of D-positive (ccDEE) red cells injected i.v., together with 1 microgram anti-D i.m., was studied in 13 D-negative male volunteers (test subjects); the red cells were labelled with 51Cr and were found to have a mean survival time of 24 d, after correction for Cr elution. Within the following 5 m...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1983.tb01997.x

    authors： Contreras M,Mollison PL

    更新日期：1983-01-01 00:00:00

  • Antenatal screening for fetal alloimmune thrombocytopenia: the results of a pilot study.

    abstract：:Feto-maternal incompatibility for the human platelet antigen HPA-1a is an important cause of severe fetal thrombocytopenia. The incidence is 1 in 1000-2000 pregnancies, which is more common than other conditions for which screening is presently carried out. Antenatal diagnosis and management are now available, but onl...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1995.tb05152.x

    authors： Doughty HA,Murphy MF,Metcalfe P,Waters AH

    更新日期：1995-06-01 00:00:00

  • Cytochemical localization of adenylate cyclase activity in rat bone marrow.

    abstract：:The localization of adenylate cyclase (AC) in rat bone marrow was studied at the ultrastructural level using a cytochemical technique with adenosine triphosphate (ATP) as substrate. The strongest precipitate occurred around cytoplasmic membranes of cells of granulocytic lineage. Around cells of erythrocytic lineage, a...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1984.tb03988.x

    authors： Krzysztofowicz E,Dabrowski Z

    更新日期：1984-11-01 00:00:00

  • Long-term study of chimaerism in bone marrow transplantation recipients for severe aplastic anaemia.

    abstract：:We used minisatellite probes to analyse by DNA fingerprints the long-term engraftment (median 4.3 years, range 1-2) of 21 bone marrow transplantation recipients for severe aplastic anaemia. Patients received their graft from histocompatible siblings. They were conditioned with cyclophosphamide (150 mg/kg) and a 6GY th...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1989.tb06313.x

    authors： Keable H,Bourhis JH,Brison O,Lehn P,Schenmetzler C,Devergie A,Gluckman E

    更新日期：1989-04-01 00:00:00

  • CD99 expression is strongly associated with clinical outcome in children with B-cell precursor acute lymphoblastic leukaemia.

    abstract：:Our study aimed to determine the expression pattern and clinical relevance of CD99 in paediatric B-cell precursor acute lymphoblastic leukaemia (BCP-ALL). Our findings demonstrate that high expression levels of CD99 are mainly found in high-risk BCP-ALL, e.g. BCR-ABL1 and CRLF2Re/Hi, and that high CD99 mRNA levels are...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/bjh.15683

    authors： Chen D,Camponeschi A,Wu Q,Gerasimcik N,Li H,Shen X,Tan Y,Sjögren H,Nordlund J,Lönnerholm G,Abrahamsson J,Fogelstrand L,Mårtensson IL

    更新日期：2019-02-01 00:00:00

  • Production of colony stimulating activity in mixed mononuclear cell culture.

    abstract：:Culture medium was harvested after co-incubation of mononuclear cells collected and pooled from the peripheral blood of two different normal donors and was tested for colony-stimulating activity (CSA) in agar culture. With bone marrow from normal donors or peripheral blood from patients with chronic granulocytic leuka...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1980.tb07144.x

    authors： Hellmann A,Th'ng KH,Goldman JM

    更新日期：1980-06-01 00:00:00

  • High efficacy with five days schedule of oral fludarabine phosphate and cyclophosphamide in patients with previously untreated chronic lymphocytic leukaemia.

    abstract：:A multicentre single-arm study testing the efficacy and toxicity of the oral combination of fludarabine and cyclophosphamide (FC) over 5 d in 75 patients with untreated B cell-chronic lymphocytic leukaemia. Oral FC demonstrated high efficacy with overall (OR) and complete response (CR) rates of 80% and 53%, respective...

    journal_title：British journal of haematology

    pub_type： 杂志文章,多中心研究

    doi：10.1111/j.1365-2141.2008.07309.x

    authors： Cazin B,Divine M,Leprêtre S,Travade P,Tournilhac O,Delmer A,Jaubert J,Feugier P,Dreyfus B,Mahé B,Grosbois B,Maloisel F,Eghbali H,Dumontet C,Bénichou J,Guibon O,Leleu X,Leporrier M,Maloum K

    更新日期：2008-10-01 00:00:00

  • A novel immunogenic CS1-specific peptide inducing antigen-specific cytotoxic T lymphocytes targeting multiple myeloma.

    abstract：:The CS1 antigen provides a unique target for the development of an immunotherapeutic strategy to treat patients with multiple myeloma (MM). This study aimed to identify HLA-A2(+) immunogenic peptides from the CS1 antigen, which induce peptide-specific cytotoxic T lymphocytes (CTL) against HLA-A2(+) MM cells. We iden...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.2012.09111.x

    authors： Bae J,Song W,Smith R,Daley J,Tai YT,Anderson KC,Munshi NC

    更新日期：2012-06-01 00:00:00

  • Reduced-intensity conditioning followed by T-cell depleted allogeneic stem cell transplantation for patients with chronic myeloid leukaemia and minimal residual disease at the time of transplant: high risk of molecular relapse.

    abstract：:A pilot trial was initiated for chronic myeloid leukaemia patients, which employed imatinib for remission induction, followed by reduced-intensity conditioning and an in vivo T-cell depleted graft. Out of nine patients, six experienced a molecular relapse and one patient had a haematological relapse at a median interv...

    journal_title：British journal of haematology

    pub_type： 临床试验,杂志文章

    doi：10.1111/j.1365-2141.2006.06404.x

    authors： Ringhoffer M,Harsdorf Sv,Schmitt M,Wiesneth M,Zenz T,Stilgenbauer S,Greiner J,Döhner K,Marx M,Döhner H,Bunjes D

    更新日期：2007-01-01 00:00:00

  • Genetic polymorphism of the major regulatory element HS-40 upstream of the human alpha-globin gene cluster.

    abstract：:The highly conserved 350-bp major regulatory element HS-40 (or alphaMRE) upstream of the human alpha-globin gene cluster is involved in the regulation of alpha-globin gene expression. The study of alphaMRE differences between human populations and the evolution of alphaMRE sequences in mammals may lead to a better und...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2141.2002.03917.x

    authors： Harteveld CL,Muglia M,Passarino G,Kielman MF,Bernini LF

    更新日期：2002-12-01 00:00:00

  • Immunodeficiency and IL-6 production by peripheral blood monocytes in multicentric Castleman's disease.

    abstract：:To study the pathogenesis of multicentric Castleman's disease (MCD), IL-6 producing cells and immune function were investigated in four MCD patients. The expression of IL-6 mRNA in one MCD lymph node was analysed by in situ hybridization. IL-6 mRNA expressing cells were scattered in the interfollicular areas and did n...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1994.tb04777.x

    authors： Ishiyama T,Nakamura S,Akimoto Y,Koike M,Tomoyasu S,Tsuruoka N,Murata Y,Sato T,Wakabayashi Y,Chiba S

    更新日期：1994-03-01 00:00:00

  • The Bcl-2 family member Bfl-1/A1 is strongly repressed in normal and malignant plasma cells but is a potent anti-apoptotic factor for myeloma cells.

    abstract：:Terminal B-cell differentiation is a multi-step process, from short-lived plasmablasts to mature long-lived plasma cells (PC). The anti-apoptotic Bcl-2 family member Bfl-1/A1 plays a critical role in the survival of mature B cells. However, its potential involvement at the later stages of B-cell development remains hi...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.2004.04908.x

    authors： Tarte K,Jourdan M,Veyrune JL,Berberich I,Fiol G,Redal N,Shaughnessy J Jr,Klein B

    更新日期：2004-05-01 00:00:00

  • CD4(+) T-cell responses to Epstein-Barr virus (EBV) latent membrane protein 1 in infectious mononucleosis and EBV-associated non-Hodgkin lymphoma: Th1 in active disease but Tr1 in remission.

    abstract：:Primary infection with Epstein-Barr virus (EBV) in childhood is usually asymptomatic, whereas infection in adolescence may result in infectious mononucleosis (IM) often followed by a fatigue syndrome. EBV latent membrane protein 1 (LMP1) is expressed in latency and in many EBV-associated tumours, including non-Hodgkin...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.2007.06765.x

    authors： Marshall NA,Culligan DJ,Johnston PW,Millar C,Barker RN,Vickers MA

    更新日期：2007-10-01 00:00:00

  • Association between hyperflexibility of the thumb and an unexplained bleeding tendency: is it a rule of thumb?

    abstract：:A bleeding tendency manifested by petechiae and ecchymoses is one of the most common causes for referral of patients to haematology clinics. Vessel wall pathology is not usually considered to be a cause for deranged haemostasis, although coexistence of increased capillary fragility and joint hypermobility have been re...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2141.1998.00697.x

    authors： Kaplinsky C,Kenet G,Seligsohn U,Rechavi G

    更新日期：1998-05-01 00:00:00

  • The relationship between serum viscosity, hypervolaemia and clinical manifestations associated with circulating paraprotein.

    abstract：:In 44 of 56 patients with malignant paraproteinaemia the relationship of serum viscosity, plasma volume (PV), estimated blood volume (BV) and clinical findings was investigated and in the remaining 12 patients one or more of these parameters was studied. There was a correlation between increased PV and serum viscosity...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1978.tb01086.x

    authors： Russell JA,Powles RL

    更新日期：1978-06-01 00:00:00

  • Use of rituximab in diffuse large B-cell lymphoma in the salvage setting.

    abstract：:The addition of rituximab (R) to CHOP (cyclophosphamide, doxorubicin, vincristine, prednisone) chemotherapy was a milestone in the development of front-line therapy for diffuse large B-cell lymphoma (DLBCL). R-CHOP and equivalent rituximab-containing anthracycline-based regimens are now widely accepted as the standard...

    journal_title：British journal of haematology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2141.2008.07383.x

    authors： Gisselbrecht C

    更新日期：2008-12-01 00:00:00

  • Normal and leukaemic stem cells.

    abstract：:The blood-related cancer leukaemias were the first diseases where human cancer stem cells, or leukaemic stem cells (LSC), were isolated. The haematopoietic system is one of the best tissues for investigating cancer stem cells, because the developmental hierarchy of normal blood formation is well defined. Leukaemias ca...

    journal_title：British journal of haematology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2141.2005.05596.x

    authors： Bonnet D

    更新日期：2005-08-01 00:00:00

  • Hepatitis C virus genotypes and severity of chronic liver disease in haemophiliacs.

    abstract：:We studied the activity and stage of chronic liver disease in 45 HCV-seropositive/HIV-seronegative patients with severe haemophilia followed for at least 10 years. HCV-RNA was detected in serum in 36 patients (80%) Viraemic cases were further analysed for HCV genotypes: 10 (28%) were infected by type 1a, 10 (28%) by t...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1995.tb05373.x

    authors： Tagariello G,Pontisso P,Davoli PG,Ruvoletto MG,Traldi A,Alberti A

    更新日期：1995-11-01 00:00:00

  • Overexpression of SOCS3 is associated with decreased survival in a cohort of patients with de novo follicular lymphoma.

    abstract：:The prognostic significance of SOCS3 protein expression was determined in de novo follicular lymphomas (FL) with t(14;18) and bcl-2 overexpression. Presentation lymph nodes from 82 FL patients for whom clinical information was available were immunohistochemically segregated into SOCS3-positive (n = 42) or -negative (n...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.2006.06248.x

    authors： Krishnadasan R,Bifulco C,Kim J,Rodov S,Zieske AW,Vanasse GJ

    更新日期：2006-10-01 00:00:00