GATEWAY vectors for Agrobacterium-mediated plant transformation.

Abstract:

:Agrobacterium tumefaciens is the preferred method for transformation of a wide range of plant species. Commonly, the genes to be transferred are cloned between the left and right T-DNA borders of so-called binary T-DNA vectors that can replicate both in E. coli and Agrobacterium. Because these vectors are generally large, cloning can be time-consuming and laborious. Recently, the GATEWAY conversion technology has provided a fast and reliable alternative to the cloning of sequences into large acceptor plasmids.

journal_name

Trends Plant Sci

journal_title

Trends in plant science

authors

Karimi M,Inzé D,Depicker A

doi

10.1016/s1360-1385(02)02251-3

subject

Has Abstract

pub_date

2002-05-01 00:00:00

pages

193-5

issue

5

eissn

1360-1385

issn

1878-4372

pii

S1360-1385(02)02251-3

journal_volume

7

pub_type

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