Interleukin-1alpha and interleukin-6 enhance the antibacterial properties of cultured composite keratinocyte grafts.

Abstract:

OBJECTIVE:To determine whether the antibacterial properties of cultured composite keratinocyte grafts can be enhanced by cytokines that stimulate the innate immune response. SUMMARY BACKGROUND DATA:Use of composite grafts of cultured keratinocytes has been limited because of their susceptibility to burn wound microorganisms as a result of their lack of a vasculature and immune cells when transplanted. Moreover, use of topical antimicrobial agents is limited with these composite grafts because of cytotoxic effects. Keratinocytes, like all epithelial cells in the body, maintain a natural defense mechanism called the innate immune system. Some components of this system can be induced by cytokines. METHODS:The innate immune response of cultured composite keratinocyte grafts treated with various cytokines was assessed indirectly by measuring the levels of mRNA encoding antimicrobial peptides (human beta defensin-1 and -2, LL-37, and antileukoprotease) and antimicrobial proteins (lysozyme, bactericidal/permeability-inducing protein, and phospholipase A2) by reverse transcription-polymerase chain reaction and directly by measuring the ability of keratinocytes to inhibit the growth of added bacteria (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus). RESULTS:Treatment with interluekin-1alpha increased mRNA levels of antimicrobial peptides in keratinocytes on plastic dishes and in composite grafts. Interleukin-6 increased mRNA levels of antimicrobial proteins in composite grafts only. When added to composite grafts, both cytokines increased antibacterial activity against E. coli, P. aeruginosa, and S. aureus. Moreover, interleukin-1alpha and interleukin-6 did not impair the formation of a differentiated epidermis in vitro or after transplantation of the composite grafts. CONCLUSIONS:Treatment with interleukin-1alpha or interleukin-6 of cultured composite keratinocyte grafts stimulates the innate immune response of keratinocytes, enhances the antibacterial properties of these grafts, and may better prepare them to combat infections in contaminated burn wounds.

journal_name

Ann Surg

journal_title

Annals of surgery

authors

Erdag G,Morgan JR

doi

10.1097/00000658-200201000-00015

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

113-24

issue

1

eissn

0003-4932

issn

1528-1140

journal_volume

235

pub_type

杂志文章
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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0000000000001054

    authors: Pereira-Terra P,Deprest JA,Kholdebarin R,Khoshgoo N,DeKoninck P,Munck AA,Wang J,Zhu F,Rottier RJ,Iwasiow BM,Correia-Pinto J,Tibboel D,Post M,Keijzer R

    更新日期:2015-12-01 00:00:00

  • Microbial colonization of tumors in relation to the upper gastrointestinal tract in patients with gastric carcinoma.

    abstract::The microbial colonization of the oropharynx, the esophagus, the stomach, and the duodenum was studied in relation to the microbial flora found on tumor and gastric mucosal biopsies in 23 patients with gastric carcinoma. The tumor was colonized in all patients, and the stomach, the esophagus, the duodenum, and the gas...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-198803000-00020

    authors: Sjöstedt S,Kager L,Heimdahl A,Nord CE

    更新日期:1988-03-01 00:00:00

  • Prevention of biliary lesions that may occur during radiofrequency ablation of the liver: study on the pig.

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    journal_title:Annals of surgery

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    doi:10.1097/01.sla.0000193831.39362.07

    authors: Marchal F,Elias D,Rauch P,Zarnegar R,Leroux A,Stines J,Verhaeghe JL,Guillemin F,Carteaux JP,Villemot JP

    更新日期:2006-01-01 00:00:00

  • Major hepatectomy for the treatment of complex bile duct injury.

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    journal_title:Annals of surgery

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    doi:10.1097/SLA.0b013e31817b65f2

    authors: Laurent A,Sauvanet A,Farges O,Watrin T,Rivkine E,Belghiti J

    更新日期:2008-07-01 00:00:00

  • Fresh frozen plasma supplement to massive red blood cell transfusion.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-198510000-00010

    authors: Martin DJ,Lucas CE,Ledgerwood AM,Hoschner J,McGonigal MD,Grabow D

    更新日期:1985-10-01 00:00:00

  • Tumor progression while on chemotherapy: a contraindication to liver resection for multiple colorectal metastases?

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/01.sla.0000145964.08365.01

    authors: Adam R,Pascal G,Castaing D,Azoulay D,Delvart V,Paule B,Levi F,Bismuth H

    更新日期:2004-12-01 00:00:00

  • Hemodynamic consequences of femoral arteriovenous bovine shunts.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-197607000-00017

    authors: Fee HJ Jr,Levisman JA,Dickmeyer JP,Golding AL

    更新日期:1976-07-01 00:00:00

  • Thoracic duct in patients with multiple organ failure: no major route of bacterial translocation.

    abstract:OBJECTIVE:To determine whether translocation of bacteria or endotoxin occurred into the thoracic duct in patients with multiple organ failure (MOF). SUMMARY BACKGROUND DATA:Translocation of bacteria or endotoxin has been proposed as a causative factor for MOF in patients without an infectious focus, although it has ra...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-199901000-00017

    authors: Lemaire LC,van Lanschot JB,Stoutenbeek CP,van Deventer SJ,Dankert J,Oosting H,Gouma DJ

    更新日期:1999-01-01 00:00:00

  • Analysis of the First 100 Patients From the Syrian Civil War Treated in an Israeli District Hospital.

    abstract:OBJECTIVE:An analysis of the injuries and treatment of the first 100 patients from the Syrian civil war was conducted to monitor quality of care and outcome. SUMMARY BACKGROUND DATA:As reports of the collapse of health care systems in regions within Syria reach the media, patients find themselves crossing the border i...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0000000000001230

    authors: Biswas S,Waksman I,Baron S,Fuchs D,Rechnitzer H,Dally N,Kassis S,Hadary A

    更新日期:2016-01-01 00:00:00

  • Alterations in gut hormones after laparoscopic sleeve gastrectomy: a prospective clinical and laboratory investigational study.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0b013e31826e1846

    authors: Dimitriadis E,Daskalakis M,Kampa M,Peppe A,Papadakis JA,Melissas J

    更新日期:2013-04-01 00:00:00

  • Activating KRAS mutations and overexpression of epidermal growth factor receptor as independent predictors in metastatic colorectal cancer patients treated with cetuximab.

    abstract:OBJECTIVE:Cetuximab, a monoclonal antibody targeting epidermal growth factor receptor (EGFR), has been proven to be efficient in metastatic colorectal cancer (mCRC); however, the therapeutic response is variable and markers predictive of response are urgently required. This study was conducted to determinate the predic...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0b013e3181bc9d96

    authors: Yen LC,Uen YH,Wu DC,Lu CY,Yu FJ,Wu IC,Lin SR,Wang JY

    更新日期:2010-02-01 00:00:00

  • Pancreatic reg gene expression is inhibited during cellular differentiation.

    abstract:BACKGROUND AND OBJECTIVE:Factors that control pancreatic regenerating (reg I) gene expression are unknown, but it is believed that its expression may correspond with cellular differentiation. The authors recently demonstrated that reg I is expressed in AR42J, a rat acinar cell line whose state of differentiation can be...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-199703000-00013

    authors: Zenilman ME,Magnuson TH,Perfetti R,Chen J,Shuldiner AR

    更新日期:1997-03-01 00:00:00

  • The prevalence and prognostic value of BRAF mutation in thyroid cancer.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0b013e318148563d

    authors: Kebebew E,Weng J,Bauer J,Ranvier G,Clark OH,Duh QY,Shibru D,Bastian B,Griffin A

    更新日期:2007-09-01 00:00:00

  • Indications and outcome of pelvic exenteration for locally advanced primary and recurrent rectal cancer.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0b013e31828a0d22

    authors: Bhangu A,Ali SM,Brown G,Nicholls RJ,Tekkis P

    更新日期:2014-02-01 00:00:00

  • Isolated limb perfusion with tumor necrosis factor and melphalan for limb salvage in 186 patients with locally advanced soft tissue extremity sarcomas. The cumulative multicenter European experience.

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    journal_title:Annals of surgery

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1097/00000658-199612000-00011

    authors: Eggermont AM,Schraffordt Koops H,Klausner JM,Kroon BB,Schlag PM,Liénard D,van Geel AN,Hoekstra HJ,Meller I,Nieweg OE,Kettelhack C,Ben-Ari G,Pector JC,Lejeune FJ

    更新日期:1996-12-01 00:00:00

  • Cryopreservation of isolated primary rat hepatocytes: enhanced survival and long-term hepatospecific function.

    abstract:OBJECTIVE:To investigate the long-term effect of cryopreservation on hepatocyte function, as well as attempt to improve cell viability and function through the utilization of the hypothermic preservation solution, HypoThermosol (HTS), as the carrier solution. SUMMARY BACKGROUND DATA:Advances in the field of bioartific...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/01.sla.0000149303.48692.0f

    authors: Sosef MN,Baust JM,Sugimachi K,Fowler A,Tompkins RG,Toner M

    更新日期:2005-01-01 00:00:00

  • Influence of site of origin and mucin production on survival in ampullary carcinoma.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-198908000-00006

    authors: Dawson PJ,Connolly MM

    更新日期:1989-08-01 00:00:00

  • Improving the AJCC/TNM staging for adenocarcinomas of the appendix: the prognostic impact of histological grade.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0b013e318269d680

    authors: Overman MJ,Fournier K,Hu CY,Eng C,Taggart M,Royal R,Mansfield P,Chang GJ

    更新日期:2013-06-01 00:00:00

  • Early aggressive use of fresh frozen plasma does not improve outcome in critically injured trauma patients.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0b013e31818990ed

    authors: Scalea TM,Bochicchio KM,Lumpkins K,Hess JR,Dutton R,Pyle A,Bochicchio GV

    更新日期:2008-10-01 00:00:00

  • Improvement in treatment results and long-term survival of patients with esophageal cancer: impact of chemoradiation and change in treatment strategy.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/01.sla.0000086545.45918.ee

    authors: Law S,Kwong DL,Kwok KF,Wong KH,Chu KM,Sham JS,Wong J

    更新日期:2003-09-01 00:00:00

  • A randomized trial comparing radiofrequency ablation and surgical resection for HCC conforming to the Milan criteria.

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    journal_title:Annals of surgery

    pub_type: 杂志文章,随机对照试验

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    authors: Huang J,Yan L,Cheng Z,Wu H,Du L,Wang J,Xu Y,Zeng Y

    更新日期:2010-12-01 00:00:00

  • Biologic determinants of tumor growth in healing wounds.

    abstract::The morphologic characteristics of a scar may render it an "immunologically privileged site" providing fertile ground for tumor occurrence and growth. We sought to extend this concept and to determine the effect of different stages of wound healing on tumor occurrence. Syngeneic strain-2 guinea pigs and a methylcholan...

    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-197902000-00009

    authors: Pendergrast WJ Jr,Futrell JW

    更新日期:1979-02-01 00:00:00

  • Surgical Techniques and Long-term Outcomes of Living-donor Liver Transplantation With Inferior Vena Cava Replacement Using Atriocaval Synthetic Interposition Graft for Budd-Chiari Syndrome.

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    pub_type: 信件

    doi:10.1097/SLA.0000000000002847

    authors: Yoon YI,Lee SG,Moon DB,Ahn CS,Hwang S,Kim KH,Ha TY,Song GW,Jung DH,Park GC,Kim DS,Choo SJ

    更新日期:2019-04-01 00:00:00

  • Early postoperative small bowel obstruction.

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    pub_type: 杂志文章

    doi:10.1097/00000658-198001000-00014

    authors: Quatromoni JC,Rosoff L Sr,Halls JM,Yellin AE

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    pub_type: 杂志文章

    doi:10.1097/00000658-200109000-00011

    authors: Khuri SF,Najjar SF,Daley J,Krasnicka B,Hossain M,Henderson WG,Aust JB,Bass B,Bishop MJ,Demakis J,DePalma R,Fabri PJ,Fink A,Gibbs J,Grover F,Hammermeister K,McDonald G,Neumayer L,Roswell RH,Spencer J,Turnage RH,V

    更新日期:2001-09-01 00:00:00

  • Multiple cytokines are required to induce hepatocyte nitric oxide production and inhibit total protein synthesis.

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    pub_type: 杂志文章

    doi:10.1097/00000658-199010000-00009

    authors: Curran RD,Billiar TR,Stuehr DJ,Ochoa JB,Harbrecht BG,Flint SG,Simmons RL

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    pub_type: 杂志文章

    doi:10.1097/00000658-199603000-00010

    authors: Horowitz J,Smith JL,Weber TK,Rodriguez-Bigas MA,Petrelli NJ

    更新日期:1996-03-01 00:00:00

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    pub_type: 杂志文章

    doi:10.1097/00000658-197709000-00004

    authors: Daggett WM,Guyton RA,Mundth ED,Buckley MJ,McEnany MT,Gold HK,Leinbach RC,Austen WG

    更新日期:1977-09-01 00:00:00

  • Variation in Bariatric Surgery Episode Costs in the Commercially Insured: Implications for Bundled Payments in the Private Sector.

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    pub_type: 杂志文章

    doi:10.1097/SLA.0000000000002462

    authors: Kelsall AC,Cassidy R,Ghaferi AA

    更新日期:2018-12-01 00:00:00

  • A prospective pathologic analysis using whole-mount sections of rectal cancer following preoperative combined modality therapy: implications for sphincter preservation.

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    pub_type: 杂志文章

    doi:10.1097/01.sla.0000232540.82364.43

    authors: Guillem JG,Chessin DB,Shia J,Suriawinata A,Riedel E,Moore HG,Minsky BD,Wong WD

    更新日期:2007-01-01 00:00:00