Abstract:
:Mitogen activation of mRNA decay pathways likely involves specific endoribonucleases, such as G3BP, a phosphorylation-dependent endoribonuclease that associates with RasGAP in dividing but not quiescent cells. G3BP exclusively cleaves between cytosine and adenine (CA) after a specific interaction with RNA through the carboxyl-terminal RRM-type RNA binding motif. Accordingly, G3BP is tightly associated with a subset of poly(A)(+) mRNAs containing its high-affinity binding sequence, such as the c-myc mRNA in mouse embryonic fibroblasts. Interestingly, c-myc mRNA decay is delayed in RasGAP-deficient fibroblasts, which contain a defective isoform of G3BP that is not phosphorylated at serine 149. A G3BP mutant in which this serine is changed to alanine remains exclusively cytoplasmic, whereas a glutamate for serine substitution that mimics the charge of a phosphorylated serine is translocated to the nucleus. Thus, a growth factor-induced change in mRNA decay may be modulated by the nuclear localization of a site-specific endoribonuclease such as G3BP.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Tourrière H,Gallouzi IE,Chebli K,Capony JP,Mouaikel J,van der Geer P,Tazi Jdoi
10.1128/MCB.21.22.7747-7760.2001subject
Has Abstractpub_date
2001-11-01 00:00:00pages
7747-60issue
22eissn
0270-7306issn
1098-5549journal_volume
21pub_type
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