Abstract：
:Malonyldialdehyde (MDA), a secondary product of lipid peroxidation is widely used as an indicator of lipid peroxidation. Eight of the most frequently used methods for measuring MDA have been evaluated with regard to their sensitivity and reproducibility. The sensitivity of these methods for pure MDA solution was in the order: Satoh's > Stocks and Dormandy's > Buege and Aust's > Dresel's > Slater's > Yoshioka's et al. > Yagi's > Jain's method. Whereas the sensitivity of the first four methods for erythrocyte MDA was in the order: Stocks and Dormandy's > Buege and Aust's > Satoh's > Dresel's method. The reproducibility (expressed as coefficient of variation) of these four methods for erythrocyte MDA were: 3.5%, 17.9%, 31.5% and 16.1%, respectively. These results indicate that Stocks and Dormandy's method has the highest sensitivity and an excellent reproducibility for erythrocyte MDA. Also, it was found to be simple and many samples can be treated in a relatively short time. When standard MDA (0.1-15 nmol/ml) was incubated with erythrocytes, the percentage recovery of MDA (using Stocks and Dormandy's method) has ranged from 50-85%. This result indicates that a considerable amount of MDA formed in erythrocytes probably reacts with other cell components and becomes undetectable. Despite this, the determination of MDA level remains a useful indicator of lipid peroxidation and correlates well with the degree of oxidative stress.
journal_name
Clin Hemorheol Microcircjournal_title
Clinical hemorheology and microcirculationauthors
Srour MA,Bilto YY,Juma Msubject
Has Abstractpub_date
2000-01-01 00:00:00pages
23-30issue
1eissn
1386-0291issn
1875-8622journal_volume
23pub_type
杂志文章abstract：:Inhalation of 100% oxygen in a hyperbaric chamber has been accepted as a useful treatment for patients with various pathologies who suffer from hypoxia. The oxidative effects of hyperbaric oxygen (HBO) on RBCs have been investigated in animals but there is not enough data on hemorheological parameters in patients foll...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-151952
更新日期：2016-01-01 00:00:00
abstract：:Ischemic recruitment of endothelial progenitor cells (EPCs) in involved in compensatory angiogenic in animal models, but this still needs to be substantiated in humans. We enrolled 12 patients, who underwent surgical correction of abdominal aortic aneurysm without atherosclerosis of leg arteries (n = 4) or lower limb ...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-2011-1437
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:It is difficult to assess tumors of the parotid gland preoperatively. However, it is essential for the surgeon to know which kind of tumor is present. Ultrasound is the method of choice, but there is still no reliable differential diagnostic tool for determining whether a tumor is malignant or benign. OBJEC...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-179223
更新日期：2017-01-01 00:00:00
abstract：:The hemorheological performance of mammals and human will change with the time after the blood is withdraw from the subject. The whole blood apparent viscosity, erythrocyte deformability and aggregation of some mammals and human were measured. Our results showed that the hemorheological characteristics of mice, rats, ...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Incubation of normal erythrocytes with 10 mM H2O2 has caused a loss of deformability. This loss of deformability was correlated with the extent of malonyldialdehyde, MDA (an indicator of lipid peroxidation) and alanine production (an indicator of protein degradation). The susceptibility of erythrocytes from 21 non-ins...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Development of new drugs is a sophisticated process that requires several, different methods. In our experiments we have applied two rheological models to study experimental and clinically used drugs. The antioxidant properties of several agents were estimated by erythrocyte filtration technique. The known antioxidant...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Determination of shear stresses at given shear rates allow approximation of flow curves by mathematical models and to calculate viscosities of non-Newtonian fluids. In term neonates, the mean arterial blood pressure (MAP) is markedly below that of adults, therefore rheological properties of blood play an important rol...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-152008
更新日期：2015-10-06 00:00:00
abstract：:In chronic respiratory insufficiency secondary erythrocytosis (SPC), causing pulmonary hypertension and dx ventricular insufficiency, is often noticed. An alternative therapy to phlebotomy for SPC is isovolemic large volume erythrocytapheresis performed with cell separator (CSE) in order to quickly remove a large volu...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：OBJECTIVES:As most clinical studies measure whole blood viscosity (WBV) from peripheral samples, potential differences in WBV obtained from the coronary arteries are often ignored. This study investigated differences in WBV measured from coronary artery specimens in patients with and without acute coronary syndrome (AC...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-131692
更新日期：2013-01-01 00:00:00
abstract：:Erythrocytes kept outside the blood circulation undergo progressive changes in metabolism, shape and function, which was the topic of this study. For that purpose, blood anticoagulated with either heparin, citrate or EDTA was incubated at temperatures of 5°C, 22°C or 37°C for 0 h, 24 h and 48 h, respectively. A temper...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-131682
更新日期：2014-01-01 00:00:00
abstract：:Obesity is associated with structural alterations in subcutaneous small resistance arteries. The aim of the present work is to study modifications of perforators vessels of abdominal wall and subcutaneous tissue characteristics in obese patients after massive weight loss. An anatomo-radiologic study was carried out on...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-131703
更新日期：2014-01-01 00:00:00
abstract：AIM:Use of contrast enhanced ultrasound (CEUS) in severe cases of COVID-19 infection to assess pulmonary changes near the pleura. MATERIAL AND METHODS:Bedside examinations by an experienced intensive care unit examiner using a multi-frequency probe (C1-6 MHz) with B-mode and CEUS to assess pleural-near changes in seve...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-209005
更新日期：2020-01-01 00:00:00
abstract：:Beta-thalassaemia is a hereditary hemolytic disease, in which each clinic phenotype encompasses a heterogenic group of genetic alterations resulting in beta-globin chain synthesis decrease or absence in red blood cells. Studies on beta-thalassaemia carriers suggest the existence of decreased red cell deformability. Th...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:In the present study, the role of the nucleus and its contribution to the deformability of the passive neutrophils was investigated. To determine the rheological properties of the nucleus and of the neutrophil itself, deformation tests on single neutrophil and sequestered nucleus have been performed by micropipette un...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-2010-1326
更新日期：2010-01-01 00:00:00
abstract：:We described a patient with symptomatic giant hepatic hemangioma (GHH) treated with laparoscopic guided percutaneous microwave ablation (MWA). A 58 years' old woman was referred to our hospital who presented with upper abdominal distension and appetite loss for more than 1 year. The medical history included untreated ...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-200922
更新日期：2020-10-13 00:00:00
abstract：:The aim of the study was to evaluate the possible relationship of the hemorheological disturbances with the clinical symptoms and some risk factors (RF) for cerebrovascular diseases (CVD). The study included 68 patients with CVD, 29 with transient ischemic attacks (TIA) and 39 with chronic unilateral cerebral infarcti...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Red blood cell distribution width (RDW) has been shown to be associated with disease activity in several inflammatory disorders. However only one study to show this has been conducted in patients with Behçet's disease (BD). The aim of the present study was to analyse the association of RDW with BD and its main complic...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-131744
更新日期：2014-01-01 00:00:00
abstract：:In a group of young subjects with acute myocardial infarction (AMI) (97 men and 8 women; mean age 39.6+/-5.5 years) we examined the thiobarbituric acid - reactive substances and the total antioxidant status at the initial stage and after 12 months. The same parameters were examined in a group of 55 control subjects. O...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Growth hormone deficiency (GHD) in adults results in alterations of body composition and metabolism associated with a lowered insulin sensitivity and an increased cardiovascular risk. Since hemorheologic disturbances (putative factors of vascular risk) are found in the insulin-resistance syndrome, we investigated bloo...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND:Advances in rapid-prototyping and 3D printing technologies have enhanced the possibilities in preparing designed architectures for tissue engineering applications. A major advantage in custom designing is the ability to create structures with desired mechanical properties. While the behaviour of a designed s...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-151931
更新日期：2015-01-01 00:00:00
abstract：PURPOSE:The purpose of this study was to evaluate whether image fusion with contrast enhanced ultrasound (CEUS) and CT is effective in the pre-, intra- and post-interventional management of liver lesions during microwave or radiofrequency ablation. METHODS AND MATERIALS:Fifteen patients with a single hepatocellular ca...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-2012-1598
更新日期：2012-01-01 00:00:00
abstract：:Antiangiogenic activity of curcumin on the tumor neogenesis was investigated by evaluating the density of neocapillaries induced by Hepatocellular carcinoma cells (HepG2) in mice, using intravital fluorescence videomicroscopy. Male BALB/c nude mice (20-25 g) were used, and a dorsal skin-fold chamber was implanted. Hep...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND:Glaucoma is currently attributed to two different possible pathogenetic mechanisms: mechanical (the ocular damage is induced by physical injury), and vascular (the ocular damage is sustained by ischemia of the optic nerve head). AIM:We considered the possibility that several anti-glaucoma drugs (beta-blocke...
journal_title：Clinical hemorheology and microcirculation
pub_type： 临床试验,杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:Radiofrequency ablation (RFA) has shown to be a reasonable alternative for the treatment of hepatic tumors and metastases although multiple limitations remain. Cooling effects due to larger vessels can prevent complete coverage and may lead to early tumor relapse. This preliminary in vivo pig study combines the use of...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-141886
更新日期：2014-01-01 00:00:00
abstract：:Vascular endothelial cells form a monocellular layer on blood vessel walls with an estimated mass of 1.5 kg. One of the roles of endothelial cells is to control the hemodynamics through various metabolic activities affecting homeostasis, vascular tonus, blood fluidity, coagulating properties and blood cell adhesion. I...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：OBJECTIVE:To identify the indications for CEUS in renal imaging in an interdisciplinary ultrasound department. METHODS:102 CEUS examinations of the kidney in 82 patients between September 2014 and July 2016 were analysed regarding the indication for ultrasound. CEUS was performed by one experienced sonographer agent a...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-179103
更新日期：2017-01-01 00:00:00
abstract：:The involvement of plasma nitric oxide metabolites (NO(x)) in hypertension was examined in stroke-resistant spontaneously hypertensive rats during the development of hypertension. Continuous application of a static magnetic field (SMF; a maximum magnetic flux density of 180 millitesla, a peak magnetic gradient of 133 ...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:Altitude training is frequently used by athletes to improve sea-level performance. However, the objective benefits of altitude training are controversial. This study aimed to investigate the possible alterations in hemorheological parameters in response to altitude training. Sprague Dawley rats, were divided into 6 gr...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-131711
更新日期：2014-01-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) are targeted as vehicles for cell mediated gene therapy. Here we report on a macromolecular carrier, which was designed aiming at successful targeted gene delivery into MSCs through the mediation of folate receptor and reduced cytotoxicity compared to established cationic polymer vector -...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-179209
更新日期：2017-01-01 00:00:00
abstract：:Hypercoagulable states due either to inherited or acquired thrombotic risk factors are only present in approximately half of cases of DVT, but the causes in the other half, remain unknown. The importance of biological risk factors such as hyperlipidemia, hypofibrinolysis and hemorheological alterations in the pathogen...
journal_title：Clinical hemorheology and microcirculation
pub_type：
doi：
更新日期：2002-01-01 00:00:00
