Abstract：
:LLC-PK(1) is a proximal tubular cell line derived from normal pig kidney which has a structure and function similar to those of renal proximal tubular cells and which expresses baseline levels of P-glycoprotein. We isolated by drug selection a doxorubicin-resistant cell line (LLC-PK(1)/ADR) that exhibited a multidrug-resistant phenotype; this cell line was characterized by reduced intracellular drug concentrations, an increased drug extrusion, and increased expression of a 170-kDa P-glycoprotein detected by Western blot analysis with monoclonal antibody C219. In addition, an increased expression of MDR1 mRNA was seen by reverse transcriptase-polymerase chain reaction. These results suggest that it is possible to induce the overexpression of P-glycoprotein by chronic treatment with doxorubicin in a normal cell line that physiologically expresses low levels of this protein. This multi-resistant cell line could provide an interesting model for studying the role of P-glycoprotein and the consequence of its induction in a normal tissue.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Decorti G,Rosati A,Candussio L,Giraldi T,Bartoli Klugmann Fdoi
10.1016/s0006-2952(00)00535-9subject
Has Abstractpub_date
2001-01-01 00:00:00pages
61-6issue
1eissn
0006-2952issn
1873-2968pii
S0006-2952(00)00535-9journal_volume
61pub_type
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