Abstract：
:This report describes the development and validation of quantitative microsatellite analysis (QuMA) for rapid measurement of relative DNA sequence copy number. In QuMA, the copy number of a test locus relative to a pooled reference is assessed using quantitative, real-time PCR amplification of loci carrying simple sequence repeats. Use of simple sequence repeats is advantageous because of the large numbers that are mapped precisely. In addition, all markers are informative because QuMA does not require that they be polymorphic. The utility of QuMA is demonstrated in assessment of the extent of deletions of chromosome 2 in leukemias arising in radiation-sensitive inbred SJL mice and in analysis of the association of increased copy number of the putative oncogene ZNF217 with reduced survival duration in ovarian cancer patients.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Ginzinger DG,Godfrey TE,Nigro J,Moore DH 2nd,Suzuki S,Pallavicini MG,Gray JW,Jensen RHsubject
Has Abstractpub_date
2000-10-01 00:00:00pages
5405-9issue
19eissn
0008-5472issn
1538-7445journal_volume
60pub_type
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