Quantitative and functional characterization of muscarinic receptor subtypes in insulin-secreting cell lines and rat pancreatic islets.

abstract：:RIN-m cells, cultured from a rat insulinoma, not only bind and secrete but also degrade insulin (Diabetes 1982; 31:521-31). The insulin-degrading activity resides in the cytosol and is similar to the insulin-specific proteases previously described in muscle and other tissues. It has an apparent Km of 0.15 microM for p...

journal_title：Diabetes

authors： Bhathena SJ,Timmers KI,Oie HK,Voyles NR,Recant L

更新日期：1985-02-01 00:00:00

abstract：:Specific allelic associations vary among ethnic groups. We studied the distribution of HLA-A,-B,-C, and -DR antigens in 34 Japanese juvenile-onset diabetic patients. The focus of our current work was HLS-DR antigens because there have been few studies of Japanese with this disease. A significant increase in the freque...

journal_title：Diabetes

authors： Sakurami T,Ueno Y,Nagaoka K,Kuno S,Iwaki Y,Park MS,Terasaki PI

更新日期：1982-02-01 00:00:00

abstract：:Diabetes-induced oxidative stress can lead to protein misfolding and degradation by the ubiquitin-proteasome system. This study examined protein ubiquitination in pancreatic sections from Zucker diabetic fatty rats. We observed large aggregates of ubiquitinated proteins (Ub-proteins) in insulin-expressing beta-cells a...

journal_title：Diabetes

authors： Kaniuk NA,Kiraly M,Bates H,Vranic M,Volchuk A,Brumell JH

更新日期：2007-04-01 00:00:00

abstract：:We have examined the effects of underfeeding and obesity on the density of hypothalamic melanocortin MC3 and MC4 receptors (MC3-R and MC4-R, respectively), which may mediate the hypophagic effects of alpha-melanocyte-stimulating hormone (MSH) in the rat. MC3-R and MC4-R were measured by quantitative autoradiography in...

journal_title：Diabetes

authors： Harrold JA,Widdowson PS,Williams G

更新日期：1999-02-01 00:00:00

abstract：:Activation of the sympathetic nervous system (SNS) constitutes a putative mechanism of obesity-induced insulin resistance. Thus, we hypothesized that inhibiting the SNS by using renal denervation (RDN) will improve insulin sensitivity (SI) in a nonhypertensive obese canine model. SI was measured using euglycemic-hyper...

journal_title：Diabetes

authors： Iyer MS,Bergman RN,Korman JE,Woolcott OO,Kabir M,Victor RG,Clegg DJ,Kolka C

更新日期：2016-11-01 00:00:00

abstract：:Wilson and coworkers (Wilson SB, Kent SC, Patton KT, Orban T, Jackson RA, Exley M, Porcelli S, Schatz DA, Atkinson MA, Balk SP, Strominger JL, Hafler DA: Extreme Th1 bias of invariant V alpha24J alpha Q T-cells in type 1 diabetes. Nature 391:177-181, 1998) have recently reported raised serum levels of interleukin-4 (I...

journal_title：Diabetes

authors： Redondo MJ,Gottlieb PA,Motheral T,Mulgrew C,Rewers M,Babu S,Stephens E,Wegmann DR,Eisenbarth GS

更新日期：1999-11-01 00:00:00

abstract：:Transcription factor expression fluctuates during β-cell ontogeny, and disruptions in this pattern can affect the development or function of those cells. Here we uncovered that murine endocrine pancreatic progenitors express high levels of the homeodomain transcription factor Prox1, whereas both immature and mature β-...

journal_title：Diabetes

authors： Paul L,Walker EM,Drosos Y,Cyphert HA,Neale G,Stein R,South J,Grosveld G,Herrera PL,Sosa-Pineda B

更新日期：2016-03-01 00:00:00

abstract：OBJECTIVE:Accumulation of glomerular matrix proteins is central to the pathogenesis of diabetic nephropathy, with resident mesangial cells (MCs) known to upregulate matrix protein synthesis in response to high glucose. Because activation of the GTPase RhoA has been implicated in matrix upregulation, we studied its role...

journal_title：Diabetes

authors： Peng F,Wu D,Gao B,Ingram AJ,Zhang B,Chorneyko K,McKenzie R,Krepinsky JC

更新日期：2008-06-01 00:00:00

abstract：OBJECTIVE:Insufficient development of a new intra-islet capillary network after transplantation may be one contributing factor to the failure of islet grafts in clinical transplantation. The present study tested the hypothesis that the angiostatic factor thrombospondin-1 (TSP-1), which is normally present in islets, re...

journal_title：Diabetes

authors： Olerud J,Johansson M,Lawler J,Welsh N,Carlsson PO

更新日期：2008-07-01 00:00:00

abstract：:Diabetes-induced alterations in heme and hemoproteins, as well as its relationship to drug-mediated induction of ALA Synthase (ALA-S), were examined in female Sprague-Dawley rats. Animals were rendered diabetic by a single i.v. injection of streptozotocin (STZ, 65 mg/kg) and measurements were made at various times aft...

journal_title：Diabetes

authors： Bitar M,Weiner M

更新日期：1984-01-01 00:00:00

abstract：:The dysregulation of receptor tyrosine kinases (RTKs) in multiple cell types during chronic inflammation is indicative of their pathogenic role in autoimmune diseases. Among the many RTKs, vascular endothelial growth factor receptor (VEGFR) stands out for its multiple effects on immunity, vascularization, and cell mig...

journal_title：Diabetes

authors： Villalta SA,Lang J,Kubeck S,Kabre B,Szot GL,Calderon B,Wasserfall C,Atkinson MA,Brekken RA,Pullen N,Arch RH,Bluestone JA

更新日期：2013-08-01 00:00:00

abstract：OBJECTIVE:To examine whether the inflammatory phenotype found in obese and diabetic individuals is preserved in isolated, cultured myocytes and to assess the effectiveness of pharmacological AMP-activated protein kinase (AMPK) activation upon the attenuation of inflammation in these myocytes. RESEARCH DESIGN AND METHO...

journal_title：Diabetes

authors： Green CJ,Pedersen M,Pedersen BK,Scheele C

更新日期：2011-11-01 00:00:00

abstract：:Secondary hyperlipidemia is a major cardiovascular risk factor in individuals with type 2 diabetes. Increased hepatic production of apolipoprotein B (apoB)-containing lipoproteins contributes to the elevated plasma levels, but the mechanism is poorly understood. Recent results have established that microsomal triglyce...

journal_title：Diabetes

authors： Bartels ED,Lauritsen M,Nielsen LB

更新日期：2002-04-01 00:00:00

abstract：:Streptozocin-induced diabetic (STZ-D) mice have reduced brain concentrations of tryptophan, a precursor substance for 5-hydroxytryptamine, and show lengthened immobility in Porsolt's swim test, a putative animal model of depression. This study investigated whether tryptophan affects behavior in Porsolt's swim test in ...

journal_title：Diabetes

authors： Hilakivi-Clarke LA

更新日期：1991-12-01 00:00:00

abstract：:G(M), the muscle-specific glycogen-targeting subunit of protein phosphatase 1 (PP1) targeted to the sarcoplasmic reticulum, was proposed to regulate recovery of glycogen in exercised muscle, whereas mutation truncation of its COOH-terminal domain is known to be associated with type 2 diabetes. Here, we demonstrate dif...

journal_title：Diabetes

authors： Lerín C,Montell E,Nolasco T,Clark C,Brady MJ,Newgard CB,Gómez-Foix AM

更新日期：2003-09-01 00:00:00

abstract：:Evidence exists for an essential role of beta-cell apoptosis in the pathology of type 1 and type 2 diabetes. Current methods for diabetes-associated apoptosis detection, however, suffer the drawbacks of relying on in situ-based strategies. In this study, we attempted to measure, both in vitro and ex vivo, levels of be...

journal_title：Diabetes

authors： Medarova Z,Bonner-Weir S,Lipes M,Moore A

更新日期：2005-06-01 00:00:00

abstract：:The T-type Ca2+ channel is an important determinant of electrical activity and of Ca2+ influx in rat and human pancreatic beta-cells. We have identified and sequenced a cDNA encoding a T-type Ca2+ channel alpha1-subunit derived from INS-1, the rat insulin-secreting cell line. The sequence of the cDNA indicates a prote...

journal_title：Diabetes

authors： Zhuang H,Bhattacharjee A,Hu F,Zhang M,Goswami T,Wang L,Wu S,Berggren PO,Li M

更新日期：2000-01-01 00:00:00

abstract：:Evidence in rodents suggests that islet pretreatment to reduce islet immunogenicity will also require some form of immunosuppression of the recipient for islet allograft acceptance in highly reactive donor-recipient pairs. We attempted to ascertain whether outbred dogs would also require treatment of both donor islets...

journal_title：Diabetes

authors： Alejandro R,Latif Z,Noel J,Shienvold FL,Mintz DH

更新日期：1987-03-01 00:00:00

abstract：:Diabetes diagnostic therapy and research would strongly benefit from noninvasive accurate imaging of the functional β-cells in the pancreas. Here, we developed an analysis of functional β-cell mass (BCM) by measuring manganese (Mn(2+)) uptake kinetics into glucose-stimulated β-cells by T1-weighted in vivo Mn(2+)-media...

journal_title：Diabetes

authors： Meyer A,Stolz K,Dreher W,Bergemann J,Holebasavanahalli Thimmashetty V,Lueschen N,Azizi Z,Khobragade V,Maedler K,Kuestermann E

更新日期：2015-06-01 00:00:00

abstract：:This is the third in a series of Perspectives on intracellular signaling pathways coupled to proliferation in pancreatic β-cells. We contrast the large knowledge base in rodent β-cells with the more limited human database. With the increasing incidence of type 1 diabetes and the recognition that type 2 diabetes is als...

journal_title：Diabetes

authors： Stewart AF,Hussain MA,García-Ocaña A,Vasavada RC,Bhushan A,Bernal-Mizrachi E,Kulkarni RN

更新日期：2015-06-01 00:00:00

abstract：:Progression to clinical type 1 diabetes varies among children who develop β-cell autoantibodies. Differences in autoantibody patterns could relate to disease progression and etiology. Here we modeled complex longitudinal autoantibody profiles by using a novel wavelet-based algorithm. We identified clusters of similar ...

journal_title：Diabetes

authors： Endesfelder D,Zu Castell W,Bonifacio E,Rewers M,Hagopian WA,She JX,Lernmark Å,Toppari J,Vehik K,Williams AJK,Yu L,Akolkar B,Krischer JP,Ziegler AG,Achenbach P,TEDDY Study Group.

更新日期：2019-01-01 00:00:00

abstract：OBJECTIVE:Insulin resistance and other features of the metabolic syndrome have been causally linked to adipose tissue macrophages (ATMs) in mice with diet-induced obesity. We aimed to characterize macrophage phenotype and function in human subcutaneous and omental adipose tissue in relation to insulin resistance in obe...

journal_title：Diabetes

authors： Wentworth JM,Naselli G,Brown WA,Doyle L,Phipson B,Smyth GK,Wabitsch M,O'Brien PE,Harrison LC

更新日期：2010-07-01 00:00:00

abstract：OBJECTIVE:Adiponectin is one of several important metabolically active cytokines secreted from adipose tissue. Epidemiologic studies have associated low-circulating levels of this adipokine with multiple metabolic disorders including obesity, insulin resistance, type 2 diabetes, and cardiovascular disease. To investiga...

journal_title：Diabetes

authors： Luo N,Liu J,Chung BH,Yang Q,Klein RL,Garvey WT,Fu Y

更新日期：2010-04-01 00:00:00

abstract：:The effects of ethanol, acetaldehyde, and the two novel metabolites of ethanol 2,3-butanediol and 1,2-propanediol on basal and insulin-stimulated adipocyte lipogenesis and glucose oxidation in vitro were investigated. Whereas ethanol and acetaldehyde inhibited both processes at concentrations far greater than those fo...

journal_title：Diabetes

authors： Lomeo F,Khokher MA,Dandona P

更新日期：1988-07-01 00:00:00

abstract：:Therapeutic engineering of glucagon-like peptide 1 (GLP-1) has enabled development of new medicines to treat type 2 diabetes. These injectable analogs achieve robust glycemic control by increasing concentrations of "GLP-1 equivalents" (∼50 pmol/L). Similar levels of endogenous GLP-1 occur after gastric bypass surgery,...

journal_title：Diabetes

authors： Briere DA,Bueno AB,Gunn EJ,Michael MD,Sloop KW

更新日期：2018-02-01 00:00:00

abstract：OBJECTIVE:We recently showed that leptin has powerful central nervous system (CNS)-mediated antidiabetic and cardiovascular actions. This study tested whether the CNS melanocortin system mediates these actions of leptin in diabetic rats. RESEARCH DESIGN AND METHODS:A cannula was placed in the lateral ventricle of Spra...

journal_title：Diabetes

authors： da Silva AA,do Carmo JM,Freeman JN,Tallam LS,Hall JE

更新日期：2009-08-01 00:00:00

abstract：:Site differences in adipose tissue function may have implications for insulin-resistant conditions. In mature adipose tissue, subcutaneous adipocytes have higher leptin secretion, similar tumor necrosis factor (TNF)-alpha secretion, and lower catecholamine-stimulated lipolysis as compared with omental adipocytes. In t...

journal_title：Diabetes

authors： van Harmelen V,Dicker A,Rydén M,Hauner H,Lönnqvist F,Näslund E,Arner P

更新日期：2002-07-01 00:00:00

abstract：:Several recent reports claim the generation of insulin-producing cells from embryonic stem cells via the differentiation of progenitors that express nestin. Here, we investigate further the properties of these insulin-containing cells. We find that although differentiated cells contain immunoreactive insulin, they do ...

journal_title：Diabetes

authors： Hansson M,Tonning A,Frandsen U,Petri A,Rajagopal J,Englund MC,Heller RS,Håkansson J,Fleckner J,Sköld HN,Melton D,Semb H,Serup P

更新日期：2004-10-01 00:00:00

abstract：:Insulin resistance (IR) is a harbinger of type 2 diabetes (T2D) and partly determined by genetic factors. However, genetically regulated mechanisms of IR remain poorly understood. Using gene expression, genotype, and insulin sensitivity data from the African American Genetics of Metabolism and Expression (AAGMEx) coho...

journal_title：Diabetes

authors： Sharma NK,Chuang Key CC,Civelek M,Wabitsch M,Comeau ME,Langefeld CD,Parks JS,Das SK

更新日期：2019-07-01 00:00:00

abstract：:One hundred fifty-four selected patients with nonketotic diabetes diagnosed between the ages of 35 and 75 yr and treated with diet or oral hypoglycemic agents for at least 1 yr were investigated for parameters of glycemic control (weight loss, blood glucose, and glycosylated hemoglobin), islet cell function (fasting a...

journal_title：Diabetes

authors： Groop LC,Bottazzo GF,Doniach D

更新日期：1986-02-01 00:00:00