Abstract：
:Unique patient-specific tumor antigens may constitute the dominant antigens in the antitumor immune response. Hence, vaccination with the patient's own repertoire of tumor antigens may offer a superior strategy to elicit protective immunity. We have shown previously that dendritic cells transfected with mRNA isolated from tumor cells stimulate potent CTL responses and engender protective immunity in tumor-bearing mice. In the current study, we demonstrate that tumor mRNA, isolated from murine tumor cell lines or from primary human tumor cells microdissected from frozen tissue sections, can be amplified without loss of function. This study provides the foundations for an effective and broadly applicable treatment that does not require the characterization of the relevant antigenic profile in each patient and will not be limited by tumor tissue availability for antigen preparation.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Boczkowski D,Nair SK,Nam JH,Lyerly HK,Gilboa Esubject
Has Abstractpub_date
2000-02-15 00:00:00pages
1028-34issue
4eissn
0008-5472issn
1538-7445journal_volume
60pub_type
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