Abstract:
:We have shown previously that bis(N,N-dimethylhydroxamido)hydroxooxovanadate (DMHV) is an excellent reversible inhibitor of protein tyrosine phosphatase (PTP) in vitro. DMHV does not carry a charge under physiological pH conditions and is anticipated to permeate cell membranes more easily than vanadate. In the present study, the efficacy of DMHV as a PTP inhibitor in intact cells was compared with that of vanadate by measuring phosphotyrosine levels in various cells treated with these compounds. DMHV was more effective in increasing both the phosphotyrosine levels of various proteins in 3T3L1 fibroblasts and the level of insulin-receptor phosphorylation in CHO cells overexpressing the human insulin receptor. DMHV was about 10- to 20-fold more effective than vanadate in increasing glucose transport and glycogen synthesis in 3T3L1 adipocytes. DMHV, unlike vanadate, also inhibited PTP in Jurkat cells. The implications of these observations are discussed.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Cuncic C,Desmarais S,Detich N,Tracey AS,Gresser MJ,Ramachandran Cdoi
10.1016/s0006-2952(99)00284-1subject
Has Abstractpub_date
1999-12-15 00:00:00pages
1859-67issue
12eissn
0006-2952issn
1873-2968pii
S0006-2952(99)00284-1journal_volume
58pub_type
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