Abstract：
:Primitive haemopoietic cells are required for studies in both the clinical and research fields and a number of systems have been developed to facilitate isolation of these haemopoietic cell populations. We have analysed the results from several European centres using positive selection of CD34+ cells from haemopoietic tissues (n = 110). Four selection techniques including immunoaffinity columns (Ceprate LC), immunomagnetic beads (Dynabeads, Baxter Isolex 50) and submicroscopic magnetic beads (MACS) were used and the selected CD34+ cells were assessed for purity, yield and enrichment of colony-forming cells (CFC). The mean purities for all samples ranged from 68.4-78.4% for MACS, 33.9-69.9% for Dynabeads, 46.9-66.8% for Ceprate LC and 43.2-65% for Baxter Isolex 50. Yields were variable with all techniques. On average CFC enrichment using the immunoaffinity columns was greater than that observed for the other systems. Some techniques appear to be problematic and may require further expertise to improve the results. Nevertheless, the study demonstrates that highly purified CD34+ cells can be isolated from various haemopoietic sources, though yield and CFC enrichment varies significantly depending on the technique selected. This extends our previous report indicating that not all selection methods generate similar results and that there are differences in the purity, number and colony-forming ability of the cells recovered.
journal_name
Bone Marrow Transplantjournal_title
Bone marrow transplantationauthors
de Wynter EA,Ryder D,Lanza F,Nadali G,Johnsen H,Denning-Kendall P,Thing-Mortensen B,Silvestri F,Testa NGdoi
10.1038/sj.bmt.1701789subject
Has Abstractpub_date
1999-06-01 00:00:00pages
1191-6issue
11eissn
0268-3369issn
1476-5365journal_volume
23pub_type
杂志文章,多中心研究abstract：:Hematopoietic SCT is currently the only curative therapy for a range of benign inherited and acquired primary hematologic disorders in children, including BM failure syndromes and hemoglobinopathies. The preferred HLA-matched sibling donor is available for only about 25% of such children. However, there has been subst...
journal_title：Bone marrow transplantation
pub_type： 杂志文章,评审
doi：10.1038/bmt.2015.1
更新日期：2015-05-01 00:00:00
abstract：:High-dose cyclophosphamide (HD-CY; 7 g/m2) was administered to patients suffering from high risk multiple myeloma (MM). The safety of this procedure, the recirculation and collection of peripheral blood stem cells (PBSC) and the effect of rhGM-CSF and HD-CY were studied. Group I patients (n = 21) were treated with HD-...
journal_title：Bone marrow transplantation
pub_type： 临床试验,杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：:A 6-year-old boy with the severe form of the leukocyte adhesion deficiency syndrome (LAD) received a transplant of cord blood (CBT) from his HLA-identical brother. The donor was proved healthy by successful prenatal diagnosis. CBT was performed after conditioning with etoposide, busulfan and cyclophosphamide. After he...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:A 5-year-old girl with Ph-positive chronic myelogenous leukemia, who underwent umbilical cord blood transplantation, developed two episodes of electrical status epilepticus while receiving tacrolimus (FK506) then cyclosporin A (CsA), as treatment against graft-versus-host disease. MRI including diffusion weighted MR i...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：10.1038/sj.bmt.1704168
更新日期：2003-09-01 00:00:00
abstract：:Hepatic venocclusive disease (VOD) is a common toxicity associated with myeloablative chemotherapy or chemoradiotherapy used to prepare patients for stem cell transplantation. A sizable proportion of patients who develop VOD die. It is clear that injury to endothelial cells and hepatocytes in zone 3 of the liver acinu...
journal_title：Bone marrow transplantation
pub_type： 杂志文章,评审
doi：10.1038/sj.bmt.1703014
更新日期：2001-06-01 00:00:00
abstract：:There exists limited information about the usefulness of hemopoietic stem cell transplantation (HSCT) for the treatment of patients with refractory Langerhans cell histiocytosis (LCH). We report here four Japanese pediatric patients with multisystem LCH disease who underwent HSCT between 1994 and 1997. Two of the four...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：10.1038/sj.bmt.1702001
更新日期：1999-10-01 00:00:00
abstract：:We report here a 6-month-old boy with severe congenital neutropenia (SCN) successfully treated by cord blood stem cell transplantation (CBSCT) from an unrelated donor. He had recurrent life-threatening respiratory infection due to severe neutropenia that was refractory to recombinant human granulocyte colony-stimulati...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：10.1038/sj.bmt.1704439
更新日期：2004-05-01 00:00:00
abstract：:The aim of this study was to define factor(s) influencing fetal erythropoiesis following bone marrow transplantation. Thirty-one transplanted patients (14 males, 17 females) were studied. The underlying diseases were chronic myelogenous leukaemia (CML, 18 patients), acute myeloblastic leukaemia (AML, 7 patients) and a...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：:Management of iatrogenic gonadal reproductive failure and sexual morbidity assumes a priority, especially in young recipients of high-dose chemotherapy and stem cell transplantation (SCT). Hormone replacement treatment (HRT) is beneficial for correction of sexual symptoms and osteoporosis in both sexes, especially in ...
journal_title：Bone marrow transplantation
pub_type： 杂志文章,评审
doi：10.1038/sj.bmt.1703721
更新日期：2002-11-01 00:00:00
abstract：:We evaluated a non-radiation containing preparative regimen for persons with myeloma receiving bone marrow (BM) or blood cell transplants. Twenty-three adults with advanced multiple myeloma (15 responsive to chemotherapy, 8 resistant) received cyclophosphamide 120 mg/kg and busulfan 14-16 mg/kg followed by the infusio...
journal_title：Bone marrow transplantation
pub_type： 临床试验,杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:Cord blood (CB) transplantation is an alternate source of human hematopoietic progenitor cells for allogeneic stem cell transplantation in children and adolescents with both malignant and nonmalignant diseases. Current limitations included delay in hematopoietic reconstitution, increased incidence of primary graft fai...
journal_title：Bone marrow transplantation
pub_type： 杂志文章,评审
doi：10.1038/bmt.2015.196
更新日期：2016-01-01 00:00:00
abstract：:The use of hematopoietic stem cells for in utero transplantation to create permanent hematochimerism represents a new concept in fetal therapy, although this approach has provided heterogeneous results. In this paper we have undertaken molecular, phenotypic and functional studies aimed at identifying the presence of f...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：10.1038/sj.bmt.1702108
更新日期：2000-01-01 00:00:00
abstract：:In a 34-year-old patient toxoplasma retinitis/encephalitis developed 9 months after bone marrow transplantation. The BMT was complicated with a serious GVHD. Although she initially responded well to antibiotic therapy she died 2 years after BMT due to progressive infection. ...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：10.1038/sj.bmt.1701128
更新日期：1998-03-01 00:00:00
abstract：:In allo-stem cell transplantation (SCT), it is unclear whether donor-specific anti-HLA Abs (DSAs) can actually mediate graft rejection or if they are simply surrogate markers for the cellular immunity that causes graft rejection. Here, we first analyzed a case of cord blood allograft rejection in which DSA and cytotox...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：10.1038/bmt.2015.119
更新日期：2015-09-01 00:00:00
abstract：:The optimal timing for recombinant human (rh)G-CSF administration after chemotherapy for PBSC mobilization has not yet been determined. In this study, we compared two different time schedules of rhG-CSF; 4th (early) vs 7th day (late), in 48 consecutive patients with multiple myeloma and lymphoma undergoing PBSC mobili...
journal_title：Bone marrow transplantation
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1038/bmt.2009.161
更新日期：2009-12-01 00:00:00
abstract：:Invasive fungal infections (IFI) with substantial mortality constitute an increasing problem among BMT patients. From 1986 to 1996 148 children underwent BMT, and are included in a retrospective analysis of the incidence, risk factors and outcome of IFI. By histopathology or culture-proven IFI (Candida, 10; Aspergillu...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：10.1038/sj.bmt.1702654
更新日期：2000-11-01 00:00:00
abstract：:Six hundred and fifteen centers from 45 countries reported a total 30,293 HSCT to this 2008 EBMT survey with 26,810 first transplants (40% allogeneic, 60% autologous). This corresponds to an increase of 7% for the allogeneic and 3% for the autologous HSCT. Main indications were leukemias (32%; 89% allogeneic); lymphom...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：10.1038/bmt.2010.69
更新日期：2011-02-01 00:00:00
abstract：:Neutrophil and platelet engraftment times are significantly shorter in patients undergoing PBPCT compared with ABMT. The explanation for this is unclear. The reticulated platelet percentage (RP%) has been established as a measure of bone marrow platelet production. Using this measurement we have followed thrombopoiesi...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：:The transfer of cytotoxic effector cells reduces the risk of relapse after allogeneic BMT. Two murine leukemia cell lines, A20 (B lymphocytic) and WEHI-3 (myelomonocytic), were used to investigate antileukemic effector mechanisms operating independently from graft-versus-host disease (GVHD). Different results were obt...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:The pathogenesis of acute graft-versus-host disease (aGVHD) includes tumor necrosis factor-alpha (TNFalpha) expression by macrophages and T cells. However, the temporal comparison of donor vs host cells to TNFalpha expression in response to irradiation conditioning and alloreactivity has not been reported. This study ...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：10.1038/sj.bmt.1704362
更新日期：2004-02-01 00:00:00
abstract：:We report the case of a 4-year-old female with high-risk ALL in first CR who received a BMT from an 11-month-old matched sibling treated with G-CSF in order to obtain an adequate number of mononuclear cells in a limited volume of bone marrow. The absence of toxicity, efficacy of the procedure and quality of the post-t...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
abstract：:Acute graft-versus-host disease (GVHD) is the most important complication of allogeneic bone marrow transplantation. We investigated the possibility of predicting severe acute GVHD using plasma interleukin-10 levels in 31 patients who underwent allogeneic bone marrow transplantation. In patients with acute GVHD, the i...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：10.1038/sj.bmt.1702010
更新日期：1999-11-01 00:00:00
abstract：:Immunotherapy in the form of donor lymphocyte infusions in early-phase relapse might be advantageous as it induces a higher response, but this may be offset by increased toxicity, especially during the early period after transplantation. Among 45 consecutive patients receiving an allograft for CML, 13 patients were di...
journal_title：Bone marrow transplantation
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/sj.bmt.1704386
更新日期：2004-03-01 00:00:00
abstract：:The aim of the present study was to assess growth, final height, growth hormone (GH) secretion and growth factors after BMT including TBI in childhood. The median age of the 25 participants was 11.3 years at BMT, and a median of 7.5 years had elapsed since BMT. The median height standard deviation score (SDS) declined...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:Results in 164 patients who underwent allogeneic marrow transplantation following busulfan and cyclophosphamide over a 15 year period were analyzed. Age (median 37, range 14-66 years) did not significantly affect the incidence of graft-versus-host disease (GVHD), but patients who received methotrexate with cyclosporin...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：10.1038/sj.bmt.1702689
更新日期：2000-11-01 00:00:00
abstract：:The use of etoposide (VP-16) for stem cell mobilization has been reported as a significant risk factor for the development of therapy-related myelodysplasia/therapy-related AML (tMDS/tAML) after transplantation. We compared the safety and effectiveness of VP-16+G-CSF (VP+G) to G-CSF alone for PBPC mobilization in pati...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：10.1038/bmt.2011.73
更新日期：2012-02-01 00:00:00
abstract：:An unblinded, historical controlled study of 49 bone marrow transplant (BMT) patients was carried out in our institution to assess the effect of oral pentoxifylline (PTX) on BMT regimen related toxicity (RRT). Twenty-eight consecutively treated BMT patients (17 allogeneic, 11 autologous) were entered into the PTX trea...
journal_title：Bone marrow transplantation
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1994-02-01 00:00:00
abstract：:To elucidate factors contributing to the effectiveness of allogeneic hematopoietic stem cell transplantation (alloHCT) in high-risk CLL, immune interventions, GvHD and clinical outcome of 77 consecutive patients allografted for CLL were analyzed. Immune modulation (immunosuppression tapering, rituximab-augmented donor...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：10.1038/bmt.2015.150
更新日期：2015-10-01 00:00:00
abstract：:Urine cytology was studied prospectively for the presence of human polyomavirus (HPV) in 17 consecutive patients undergoing marrow transplantation and correlated with hematuria and hemorrhagic cystitis. Of the 15 evaluable patients, nine had cytologic findings consistent with HPV infection during the first month after...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：:A case of pneumatosis intestinalis with perforation is reported in a patient after bone marrow allograft for chronic myeloid leukemia. Risk factors included the transplant, prolonged immunosuppression and neutropenia, graft-versus-host disease, extended use of corticosteroids, infection and lower gastrointestinal endo...
journal_title：Bone marrow transplantation
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
