HumCD4--validation of a STR system for forensic purposes in an Austrian Caucasian population sample.

Abstract:

:The short tandem repeat system HumCD4 was amplified by the polymerase chain reaction (PCR) on blood samples from 304 unrelated Austrian Caucasians and analyzed by horizontal, non-denaturing polyacrylamide electrophoresis. The mean exclusion chance was 0.417, the discriminating power 0.850 and the heterozygosity rate 0.628. The observed phenotype distribution is in Hardy-Weinberg equilibrium. In 100 families (200 meioses) no mutations were found. Sufficient amplification could be achieved with as little as 80 pg of high molecular weight cell-line DNA. In a degradation experiment DNA extracted from bloodstains stored for up to 28 days in a moist chamber and DNA boiled for up to 18 min could be amplified. A duplex PCR with TH01 is proposed.

journal_name

J Forensic Sci

authors

Klintschar M,Crevenna R

subject

Has Abstract

pub_date

1997-09-01 00:00:00

pages

907-10

issue

5

eissn

0022-1198

issn

1556-4029

journal_volume

42

pub_type

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