Abstract：
:Malate dehydrogenase (EC 1.1.1.37.) (MDH) was purified to apparent homogeneity from the cytosolic fraction of the protozoan Trichomonas vaginalis Donné. The four step purification included ion-exchange chromatography (DEAE-Sephacel and Q-Sepharose, elution with NaCl) and affinity chromatography (Reactive Red Agarose, elution with NADH and NaCl). The enzyme was purified about 132-fold (30.6% yield) to a specific activity of 352 units mg-1. The Km values determined at pH 7.8 (pH optimum from 7.5 to 8.3) for oxaloacetate and NADH were 16.2 microM and 10.6 microM, respectively. The MDH activity was inhibited by the substrate, decreasing to 50% at about 1 microM concentration of oxaloacetate. The reverse reaction from malate to oxaloacetate showed a pH optimum around pH 9.5. The Km for malate and NAD+ (determined at pH 7.8) were 1220 microM and 69.9 microM, respectively. SDS-PAGE analysis of the purified MDH revealed a single band with an apparent size of 34.5 kDa. The native molecular weight was estimated by HPLC gel filtration to be 60 kDa, which indicates that the T. vaginalis MDH exists as a dimer.
journal_name
Folia Parasitol (Praha)journal_title
Folia parasitologicaauthors
Drmota T,Tachezy J,Kulda Jsubject
Has Abstractpub_date
1997-01-01 00:00:00pages
103-8issue
2eissn
0015-5683issn
1803-6465journal_volume
44pub_type
杂志文章abstract：:Surface structures, particularly numerous body sculptures of the nematode M. laryngeus recovered from cattle in Cuba were studied by scanning electron microscopy. New morphological structures in form of "granulations" were found of the mouth capsule. The structure of cuticle in region of vulva opening and of bursa cut...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
abstract：:Collections of parasitic nematodes of fishes from some freshwater and brackish-water localities in Honshu, Shikoku and Hokkaido, Japan, carried out in 1996-2009, yielded the following eight species: Hysterothylacium haze (Machida, Takahashi et Masuuchi, 1978) (Anisakidae), Paraquimperia sp., Pingus sinensis Hsü, 1933 ...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2018.016
更新日期：2018-10-23 00:00:00
abstract：:External morphology of the nematode Pterygodermatites bovieri (Blanchard, 1886), a very rare parasite of bats in the Palaearctic region, was studied by scanning electron microscopy. Special attention was paid to the cephalic end structure and cuticular armament, which are of great systematic importance in this group o...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:An epidemiological analysis was carried out of more than 11 thousand cases of scabies reported in 1961-1979 in the district of Karviná, Czechoslovakia. The paper presents analyses of the incidence of scabies in different age groups of patients divided according to sex and occupation and the results of the epidemiologi...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:In Senegal, several areas provide great potential for agriculture and animal production, but African animal trypanosomosis (AAT) is one of the major constraints to the development of more effective livestock production systems. A study was conducted to assess the current situation of AAT in this country. Surveys were ...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2015.044
更新日期：2015-08-18 00:00:00
abstract：:This study was undertaken to attempt to identify correlations between microsporidial seroprevalence data in man, clinical diseases and groups of people at the risk of HIV/AIDS infection. Groups of patients were selected according to the predilection of members of the genus Encephalitozoon for nervous and kidney tissue...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Life cycle of Eimeria coecicola was studied in experimentally infected rabbits by light microscopy and by transmission and scanning electron microscopy. First and second generation meronts developed in the vermiform appendix; third and fourth generation meronts were located in the epithelium of the ileum. Gametogony d...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Sex ratio and engorged females ratio were studied in the main mosquito species populations (Aedes aegypti, Culex quinquefasciatus and partially Anopheles vagus) in the houses of Phnom Penh. Collections were carried out in 7 different localities, including area from the centre of the town to the periphery. In Ae. aegyp...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Cestodes of the family Anoplocephalidae Cholodkovsky, 1902, in their adult form, parasitize a variety of hosts, including reptiles, birds and mammals. To complete their life cycle, an intermediate host is required. This study gives a critical review of the life cycles of genera principally important to veterinary medi...
journal_title：Folia parasitologica
pub_type： 杂志文章,评审
doi：
更新日期：1998-01-01 00:00:00
abstract：:In the region of the Barun Glacier, in the vicinity of the Makalu Mountain in the Great Himalaya (Nepal) two mite species of the family Myobiidae, Protomyobia kounickyi sp. n. and Radfordia lemnina (C. L. Koch, 1841) were found on Soriculus caudatus soluensis (Gruber) and Alticola roylei (Gray) respectively at high al...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:Four laboratory-hatched European kestrels Falco tinnunculus L. were fed on laboratory mice and common voles Microtus arvalis Pallas previously inoculated with different doses of sporulated oocysts of Caryospora kutzeri Böer, 1982. Two kestrels that were fed infected mice shed C. kurtzeri oocysts 6 days after ingesting...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2001.003
更新日期：2001-01-01 00:00:00
abstract：:A survey is given of 23 species of synathropic and synbovine Diptera collected in the years 1968, 1969 and 1972 in the Mongolian People's Republic by J. Minár. Besides the faunistical comments, the species of hygienic-epidemiological or public health importance are discussed. ...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:Two squirrels aged 16 weeks and three muskrats aged 24 weeks were subcutaneously infected with a dose of 400 SMicLD50 of the extraneurally passaged "236" strain of Tahyna virus. Viremia was detected in one squirrel (48 and 96 hours post infection) and in two muskrats (24 and 48 hours p.i.). Seroconversion was demonstr...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Caryosporan oocysts were found in the feces of a diamond rattlesnake, Crotalus atrox, kept in ZOO Ustí nad Labem, Czech Republic. Comparison of oocyst structure with hitherto described Caryospora species from snakes revealed that they were Caryospora bigenetica Wacha et Christiansen, 1982. The rattlesnake, Crotalus at...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:The genome sequence of the microsporidian parasite Encephalitozoon cuniculi Levaditi, Nicolau et Schoen, 1923 contains about 2,000 genes that are representative of a non-redundant potential proteome composed of 1,909 protein chains. The purpose of this review is to relate some advances in the characterisation of this ...
journal_title：Folia parasitologica
pub_type： 杂志文章,评审
doi：10.14411/fp.2005.003
更新日期：2005-05-01 00:00:00
abstract：:Soricinia tripartita Zarnowski, 1955 is redescribed on the basis of specimens from the type host Sorex araneus Linnaeus from Lithuania, Latvia and Russia (Republic of Karelia and Republic of Komi - a new geographical record) as well as from Sorex satunini Ognev and Sorex volnuchini Ognev from Russia (Nalchik Area in t...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：2014-04-01 00:00:00
abstract：:During October 2003, a specimen of Amblyrhynchotes honckenii (Bloch, 1795) was captured at low tide, with a hand net, in a rock pool at Koppie Alleen, De Hoop Nature Reserve, South Africa. This fish was heavily parasitized by unidentified gnathiid praniza larvae, caligid copepods identified as Caligus tetrodontis Barn...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2005.037
更新日期：2005-09-01 00:00:00
abstract：:Seven trematode species were found in the bile ducts and rumen of domestic ruminants: Fasciola gigantica, F. hepatica, Gigantocotyle explanatum, G. siemense, Paramphistomum epiclitum, Fischoederius cobboldi and Gastrothylax cruminifer. The parasites caused marked pathological changes in the liver and rumen. ...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:Although the microscopic examination of stool samples remains the reference method of choice for the diagnosis of intestinal protistan infections, this method is time-consuming and requires experienced and well-trained operators. The purpose of this study was to evaluate the level of agreement between the BD MAX TM En...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2020.020
更新日期：2020-08-07 00:00:00
abstract：:A variety of conditions of cryopreservation were evaluated in order to define a single procedure for freezing the amebae of pathogenic Naegleria and Acanthamoeba. The average best conditions for freezing the three species studied were: 1 x 10(6) exponentially growing amebae/ml of freezing medium consisting of 12% dime...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The Capsalidae are monogeneans parasitizing 'skin', fins and gills of marine fishes. Some capsalids are pathogenic to cultivated fish and a few have caused epizootic events. It is a cosmopolitan family with broad host associations (elasmobranchs and teleosts, including sturgeons). Approximately 200 capsalid species ar...
journal_title：Folia parasitologica
pub_type： 杂志文章,评审
doi：10.14411/fp.2004.016
更新日期：2004-06-01 00:00:00
abstract：:We have assessed the phylogenetic status of the Leishmania genome project Friedlin reference strain by MLEE and multiprimer RAPD including a set of 9 stocks representative of the main Leishmania species and of the whole genetic diversity of the Leishmania genus. To our knowledge, the detailed genetic characterization ...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:A comparison of the behavioural peculiarities of Ixodes persulcatus Schulze, 1930 (north-western population, Russia) and Ixodes ricinus (L., 1758) from western Russia and Denmark was determined by using two methods. Method 1 involved a sojourn of ticks on vertical plastic sticks and showed that the questing behaviour ...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2000.029
更新日期：2000-01-01 00:00:00
abstract：:While studying the suslik fleas in artificial nests planted in free nature, significant changes in the population dynamics of N. setosa, C. simplex and C. orientalis (from the spring build up to the autumnal peak and the decrease of flea number until the next spring) were noted. The predominance of females in the spri...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:We confirmed, with the electron microscope, that Trichinella larvae feed directly on the host tissue during their initial development. They suck, by means of a stylet acting as a piston, all components of the muscle cell which develops into a nurse cell, into their oral cavity. The stylet is controlled by a muscle ret...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Primary amoebic meningoencephalitis (PAM) was induced in mice by intranasal inoculation of Naegleria fowleri (Singh et Das, 1970) to study the role of the blood vessels and lungs in the early and later stages in this disease. Upon culturing blood and lung tissue obtained at 24-, 36-, 48-, 72-, 96-, and 120-hour time p...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2002.035
更新日期：2002-01-01 00:00:00
abstract：:The philometrid nematode, Philometra saltatrix Ramachandran, 1973, is redescribed from male and female specimens collected from the ovaries of the marine fish (bluefish) Pomatomus saltatrix (L.) (Pomatomidae, Perciformes) from the Tuscan Sea, Italy. The light and scanning electron microscopical examination made it pos...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2008.029
更新日期：2008-09-01 00:00:00
abstract：:Based on the study of type material, two new genera of cestodes (Cyclophyllidea: Anoplocephalidae) are proposed for Paranoplocephala Lüihe, 1910 sensu lato species from African rodents. Afrojoyeuxia gen. n., proposed for A. gundii (Joyeux, 1923) comb. n. from Ctenodactylus gundi (Rothmann) (Hystricomorpha: Ctenodactyl...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：10.14411/fp.2013.051
更新日期：2013-11-01 00:00:00
abstract：:Plasmodia of a Henneguya species measuring 70-900 microns and exhibiting season-dependent stages of development were detected throughout a three-year study on gill myxosporosis of Lake Balaton pikeperch (Stizostedion lucioperca (L.)). Sixty-five out of 160 fish (41%) examined in the period of study were infected by th...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:In field and laboratory conditions new data were obtained concerning the life cycle of the nidicolous tick Ixodes laguri. The development of this tick was studied in experimental nests of the European suslik and a two-year cycle of I. laguri was demonstrated. However, the materials obtained indicate that a longer cycl...
journal_title：Folia parasitologica
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
